Optimal voltages and Signal Spread Matrix FACSymphony A5
The Core facility staff would be happy to help you plan and execute multi-parameter flow experiments,
however here are some key features that may help you do it yourself.
Choose a color that recieves little spread for markers that you are investigating double positives or weak positive
Choose a color that donates little spread for your "backbone markers", or else they will impact your entire panel
Avoid bad spillover spread combinations in your panel. If you have to use such a combination, use them on mutually exclusive markers (e.g. CD3 vs CD19)
FACSymphony Panel Builder
This spreadsheet can be used as a template for building FACSymphony panels. It includes information from the SSM and recorded stain index: FACSymphony Panel Builder