Services

The FCCF provides flow cytometry analysis and sorting of cells, cell organelles, bacteria and other micro-particles based on light scatter or fluorescent markers. In addition, the node at Radiumhospitalet offers mass cytometry analysis of cell suspensions or imaging mass cytometry of tissue/cells on slides.

Instruments:

Montebello/Radiumhospitalet:

Gaustad/Rikshospitalet:

Ullevål:

• Luminex IS 100
• Accuri C6
• FACSMelody cell sorter

Training:

The facility staff will provide training and education of the users before they are allowed to run the cytometers themselves. Please contact your local facility staff if you have any questions related to the training program.

Booking:

See our Booking page for more information on how to book DIY instruments.

Software:

In addition to vendor specific software for acquisition on the flow cytometers, we have additional software for data analysis:
- FlowJo (Gaustad, Montebello)
- SpectroFlo (Montebello)

For imaging mass cytometry:

- HistoCAT (Montebello)
- Ilastik (Montebello)
- CellProfiler (Montebello)

Biosafety:

The Flow Cytometry facility does not perform viable cell sorting on samples containing pathogens known to infect humans. Due to the fact that cell sorting generates aerosols and that cells are generally not submitted in any fixative, the biohazard risks are much greater than in analytical flow cytometry. It is therefore important to discuss any and all biohazard issues with the sort operator. BioSafety considerations also apply to the use of the analytical instruments, please discuss this with the core staff during your training.