The Flow Cytometry Core Facility has provided flow cytometry analysis and cell sorting services to interested users for 25 years.
The FCCF provides sorting of cells, cell organelles, bacteria and other microorganisms based on light scatter and/or fluorescent markers. Cells can be sorted under semi-sterile conditions and with high purity enabling the recovered cells to be cultured. It is possible to sort cells according to antigen/flourescent proitein expression, DNA content or cell function, or a combination thereof. Cells can also be sorted to retrieve sub-populations for RNA and protein extraction or for morphological analysis. Sorted cells can be collected into tubes (one to four tubes/populations) or deposited as single cells into multiwell plates or onto slides. This can be useful for cloning transfected cells, hybridomas, PCR, FISH, etc.
We also provide training and assistance for mass and fluorescence flow cytometry analysis as well as technical expertise and advice for experimental planning and design, cell preparation, analysis and data handling.
The newest additions to the core facility are the mass cytometry imaging unit for imaging of tissue/cells on slides (Hyperion, Montebello, up to 35 parameters) and the 30 parameter analytical flow cytometer (FACSymphony A5, Montebello).
The flow cytometry core facility is a non-profit facility and is supported by grants from Helse Sør-Øst, OUS and UiO, as well as nominal user fees.
Services are provided primarily for academic users from the Helse Sør-Øst region. In addition, other interested national and international academic users as well as commercial actors are encouraged to contact FCCF management/staff to contract services.
Trained users will be able to use our instruments (DIY) for a fee per hour to cover running expenses (reagents, materials and service). Sorting or analysis assistance performed by Core facility staff will be charged per hour. Detailed price information can be found on the page for each instrument.
The Flow Cytometry Core Facility at OUS divides its instrument parks between Montebello, Gaustad and Ullevål.
All rooms in K-building ("Forskningsbygget"): K03.078 and K03.080 (both East wing, "Strålingsbiologi") / K03.020 (West wing, "Kreftimmunologi"), Department of Core Facilities, Institute for Cancer Research,
The Norwegian Radium Hospital, Montebello, 0310 Oslo
Room A3.M037/ A3. M047, Institute of Pathology, Rikshospitalet, Sognsvannsveien 20, 0027 Oslo
Room 0606030, Midtblokka 6th floor
Department for Medical Biochemistry, Ullevål hospital,
Kirkeveien 166, 0450 Oslo