Overview of tissue
This sample was a tiny tissue piece that had dried in the -70°C freezer. Attempts were made to rehydrate the tissue prior to sectioning in the cryotome. The reason for this deviation from the standard protocol was that a matching lymph node (in good condition) was available. Consequently thicker slices had to be taken. 9 microtome slices, of 20 μm thickness were obtained, where 4 slices were subjected to laser capture microdissection (LCMD). The same grid of 96 (~25000 μm2) circles was used as a template for the laser capture microdissection on each slide.
Descriptive statistics of mutant fraction found in each LCMD sample.
|First Quantile (%)||8.24||9.7|
|Third Quantile (%)||25.0||59.3|
Links to 3 different approaches to visualizing the mutant fraction in the 3D volume.
Isosurface of a random spread of homoplasmic cells (wild-type and/or mutant) in each LCMD volumes.
No isosurfaces of mutant fractions were created for the two markers due condition of the tissue and the sampling.
3D bubble plot of wild-type and mutant fractions.
Marker 1 (P2F6)
Marker 2 (P5F6)