This sample had limited amount of tissue, thus only 6 consecutive 12μm microtome slides were placed on membrane suitable for laser capture microdissection (LCMD). A grid of 96 (~25000 μm2) circles was used as a template for the laser capture microdissection on each membrane slide. This sample has corresponding lymph node and liver metastases. An attempt was also made to insert a guide object (plastic string) that could be followed through the tissue (click on the picture for enlargement).
Descriptive statistics of mutant fraction found in each LCMD sample.
|Marker P1F1||Prime tumor||Liver met||Lymph node|
|First Quantile (%)||26.0||14.2||47.5|
|Third Quantile (%)||44.3||27.4||58.6|
Links to 3 different approaches to visualizing the mutant fraction in the3D volume.
Isosurfaces of a random spread of homoplasmic cells (wild-type and/or mutant) in each LCMD volumes.
Isosurfaces of mutant fractions from 5% to 95% in 5% points increments.
3D bubble plot of wild-type and mutant fractions.