Nikon ECLIPSE Ti2-E CrestOptics X-Light V3 spinning disk

The Nikon Crest system is a Nikon ECLIPSE Ti2-E inverted microscope equipped with the CrestOptics X-Light V3 spinning disk confocal module.

The Nikon Crest spinning disk microscope is suitable for imaging live cells, fixed samples, thick tissue samples, as well as organoids. This instrument has a large field of view (25 x 25 mm) and uniform illumination resulting in seamless stitching of large images.

7 laser lines allows imaging of the most common fluorophores (DAPI, FITC, TRITC, Cy5, Cy7, CFP, YFP) in either cofocal mode or in widefield mode.

In addition, the dual camera setup and Z piezo device give fast imaging of multiple channels and z-stacks with nanometer precision.

Microscope features:

  • Motorized XY stage
  • Z piezo device
  • Stage-top incubator and XL-cage with CO2 and temperature control
  • Dual camera imaging (Back-illuminated sCMOS cameras, Photometrics Kinetix)
  • Lumencor Celesta multi-line laser (405, 446, 477, 518, 546, 638, 749 nm)
  • CrestOptics X-Light V3 spinning disk (50 um pinhole)
  • Objectives:

CFI Plan Apo l D 10X NA 0.45 (Air)

CFI Plan Apo l D 20X NA 0.8 (Air)

CFI Plan Apo l D 40XC NA 0.95 (Air)

CFI Plan Apo l S 40XC Sil NA 1.25 (Silicone)

CFI Plan Apo lIR 60X NA 1.27 (Water)

CFI Plan Apo l D 60X Oil NA 1.42 (Oil)

CFI Plan Apo l D 100X Oil NA 1.45 (Oil) 

  • Tube lens changer (1.5x extra magnification)
  • Anti-vibration table
  • NIS Elements AR software
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