MODEL SYSTEMS AND METHODS
As in vitro model systems we use a large number of human cell lines derived from cancers and normal tissues, and organoids of epithelial cells grown in matrigel. As in vivo model we employ zebrafish and the fruit fly Drosophila melanogaster. We use a number of advanced genetic and molecular biology methods to turn specific gene functions on and off, and as readouts we use both biochemical assays and molecular imaging. In particular, our group has experience in advanced light and electron microscopy. Light imaging methods include confocal microscopy, SIM and STORM super-resolution microscopy, live imaging, selective plane illumination microscopy (SPIM), and high-content imaging. Electron microscopy methods include conventional plastic embedding electron microscopy, immunoelectron microscopy, electron tomography and correlative light and electron microscopy (CLEM). Our group is scientifically responsible for the core facilities in Advanced Light Microscopy and Electron Microscopy of the Institute for Cancer Research.