Ellen Skarpen

  • Scientist, core facility manager (Helse Sør-øst)
  • +47 22 78 19 05

Ellen runs the Institute's core facility for confocal microscopy

 

Publications 2019

Andersen E, Chollet ME, Baroni M, Pinotti M, Bernardi F, Skarpen E, Sandset PM, Skretting G (2019)
The effect of the chemical chaperone 4-phenylbutyrate on secretion and activity of the p.Q160R missense variant of coagulation factor FVII
Cell Biosci, 9, 69
DOI 10.1186/s13578-019-0333-8, PubMed 31467667

Goodridge JP, Jacobs B, Saetersmoen ML, Clement D, Hammer Q, Clancy T, Skarpen E, Brech A, Landskron J, Grimm C, Pfefferle A, Meza-Zepeda L, Lorenz S, Wiiger MT, Louch WE, Ask EH, Liu LL, Oei VYS, Kjällquist U, Linnarsson S, Patel S, Taskén K, Stenmark H, Malmberg KJ (2019)
Remodeling of secretory lysosomes during education tunes functional potential in NK cells
Nat Commun, 10 (1), 514
DOI 10.1038/s41467-019-08384-x, PubMed 30705279

Publications 2018

Andersen E, Chollet ME, Myklebust CF, Pinotti M, Bernardi F, Chuansumrit A, Skarpen E, Sandset PM, Skretting G (2018)
Activation of Endoplasmic Reticulum Stress and Unfolded Protein Response in Congenital Factor VII Deficiency
Thromb Haemost, 118 (4), 664-675
DOI 10.1055/s-0038-1637690, PubMed 29618153

Eng MS, Kaur J, Prasmickaite L, Engesæter BØ, Weyergang A, Skarpen E, Berg K, Rosenblum MG, Mælandsmo GM, Høgset A, Ferrone S, Selbo PK (2018)
Enhanced targeting of triple-negative breast carcinoma and malignant melanoma by photochemical internalization of CSPG4-targeting immunotoxins
Photochem Photobiol Sci, 17 (5), 539-551
DOI 10.1039/C7PP00358G, PubMed 29565434

Norum JH, Skarpen E, Brech A, Kuiper R, Waaler J, Krauss S, Sørlie T (2018)
The tankyrase inhibitor G007-LK inhibits small intestine LGR5+ stem cell proliferation without altering tissue morphology
Biol Res, 51 (1), 3
DOI 10.1186/s40659-017-0151-6, PubMed 29316982

Weyergang A, Fremstedal AS, Skarpen E, Peng Q, Mohamedali KA, Eng MS, Cheung LH, Rosenblum MG, Waltenberger J, Berg K (2018)
Light-enhanced VEGF121/rGel: A tumor targeted modality with vascular and immune-mediated efficacy
J Control Release, 288, 161-172
DOI 10.1016/j.jconrel.2018.09.005, PubMed 30217739

Publications 2017

Chollet ME, Andersen E, Skarpen E, Myklebust CF, Koehler C, Morth JP, Chuansumrit A, Pinotti M, Bernardi F, Thiede B, Sandset PM, Skretting G (2017)
Factor VII deficiency: Unveiling the cellular and molecular mechanisms underlying three model alterations of the enzyme catalytic domain
Biochim Biophys Acta Mol Basis Dis, 1864 (3), 660-667
DOI 10.1016/j.bbadis.2017.12.016, PubMed 29246447

Publications 2016

Hildonen S, Skarpen E, Halvorsen TG, Reubsaet L (2016)
Isolation and mass spectrometry analysis of urinary extraexosomal proteins
Sci Rep, 6, 36331
DOI 10.1038/srep36331, PubMed 27805059

Publications 2015

Bull-Hansen B, Berstad MB, Berg K, Cao Y, Skarpen E, Fremstedal AS, Rosenblum MG, Peng Q, Weyergang A (2015)
Photochemical activation of MH3-B1/rGel: a HER2-targeted treatment approach for ovarian cancer
Oncotarget, 6 (14), 12436-51
DOI 10.18632/oncotarget.3814, PubMed 26002552

Chollet ME, Skarpen E, Iversen N, Sandset PM, Skretting G (2015)
The chemical chaperone sodium 4-phenylbutyrate improves the secretion of the protein CA267T mutant in CHO-K1 cells trough the GRASP55 pathway
Cell Biosci, 5, 57
DOI 10.1186/s13578-015-0048-4, PubMed 26457178

Theodossiou TA, Wälchli S, Olsen CE, Skarpen E, Berg K (2015)
Deciphering the Nongenomic, Mitochondrial Toxicity of Tamoxifens As Determined by Cell Metabolism and Redox Activity
ACS Chem Biol, 11 (1), 251-62
DOI 10.1021/acschembio.5b00734, PubMed 26569462

Publications 2014

Bull-Hansen B, Cao Y, Berg K, Skarpen E, Rosenblum MG, Weyergang A (2014)
Photochemical activation of the recombinant HER2-targeted fusion toxin MH3-B1/rGel; Impact of HER2 expression on treatment outcome
J Control Release, 182, 58-66
DOI 10.1016/j.jconrel.2014.03.014, PubMed 24637464

Lund K, Bostad M, Skarpen E, Braunagel M, Kiprijanov S, Krauss S, Duncan A, Høgset A, Selbo PK (2014)
The novel EpCAM-targeting monoclonal antibody 3-17I linked to saporin is highly cytotoxic after photochemical internalization in breast, pancreas and colon cancer cell lines
MAbs, 6 (4), 1038-50
DOI 10.4161/mabs.28207, PubMed 24525727

Publications 2013

Bostad M, Berg K, Høgset A, Skarpen E, Stenmark H, Selbo PK (2013)
Photochemical internalization (PCI) of immunotoxins targeting CD133 is specific and highly potent at femtomolar levels in cells with cancer stem cell properties
J Control Release, 168 (3), 317-26
DOI 10.1016/j.jconrel.2013.03.023, PubMed 23567040

Flinder LI, Wierød L, Rosseland CM, Huitfeldt HS, Skarpen E (2013)
FAK regulates Cdk2 in EGF-stimulated primary cultures of hepatocytes
J Cell Physiol, 228 (6), 1304-13
DOI 10.1002/jcp.24287, PubMed 23168795

Liu S, Wierød L, Skarpen E, Grøsvik H, Duan G, Huitfeldt HS (2013)
EGF activates autocrine TGFα to induce prolonged egf receptor signaling and hepatocyte proliferation
Cell Physiol Biochem, 32 (3), 511-22
DOI 10.1159/000354454, PubMed 24008581

Stratford EW, Bostad M, Castro R, Skarpen E, Berg K, Høgset A, Myklebost O, Selbo PK (2013)
Photochemical internalization of CD133-targeting immunotoxins efficiently depletes sarcoma cells with stem-like properties and reduces tumorigenicity
Biochim Biophys Acta, 1830 (8), 4235-43
DOI 10.1016/j.bbagen.2013.04.033, PubMed 23643966

Publications 2012

Pedersen NM, Raiborg C, Brech A, Skarpen E, Roxrud I, Platta HW, Liestøl K, Stenmark H (2012)
The PtdIns3P-binding protein Phafin 2 mediates epidermal growth factor receptor degradation by promoting endosome fusion
Traffic, 13 (11), 1547-63
DOI 10.1111/j.1600-0854.2012.01400.x, PubMed 22816767

Publications 2011

Flinder LI, Timofeeva OA, Rosseland CM, Wierød L, Huitfeldt HS, Skarpen E (2011)
EGF-induced ERK-activation downstream of FAK requires rac1-NADPH oxidase
J Cell Physiol, 226 (9), 2267-78
DOI 10.1002/jcp.22563, PubMed 21660950

Publications 2008

Oksvold MP, Funderud A, Kvissel AK, Skarpen E, Henanger H, Huitfeldt HS, Skålhegg BS, Ørstavik S (2008)
Epidermal growth factor receptor levels are reduced in mice with targeted disruption of the protein kinase A catalytic subunit
BMC Cell Biol, 9, 16
DOI 10.1186/1471-2121-9-16, PubMed 18380891

Rosseland CM, Wierød L, Flinder LI, Oksvold MP, Skarpen E, Huitfeldt HS (2008)
Distinct functions of H-Ras and K-Ras in proliferation and survival of primary hepatocytes due to selective activation of ERK and PI3K
J Cell Physiol, 215 (3), 818-26
DOI 10.1002/jcp.21367, PubMed 18163378

Publications 2007

Skarpen E, Flinder LI, Rosseland CM, Orstavik S, Wierød L, Oksvold MP, Skålhegg BS, Huitfeldt HS (2007)
MEK1 and MEK2 regulate distinct functions by sorting ERK2 to different intracellular compartments
FASEB J, 22 (2), 466-76
DOI 10.1096/fj.07-8650com, PubMed 17928366

Wierød L, Rosseland CM, Lindeman B, Oksvold MP, Grøsvik H, Skarpen E, Huitfeldt HS (2007)
CDK2 regulation through PI3K and CDK4 is necessary for cell cycle progression of primary rat hepatocytes
Cell Prolif, 40 (4), 475-87
DOI 10.1111/j.1365-2184.2007.00451.x, PubMed 17635516

Wierød L, Rosseland CM, Lindeman B, Oksvold MP, Grøsvik H, Skarpen E, Huitfeldt HS (2007)
Activation of the p53-p21(Cip1) pathway is required for CDK2 activation and S-phase entry in primary rat hepatocytes
Oncogene, 27 (19), 2763-71
DOI 10.1038/sj.onc.1210937, PubMed 18026139

Publications 2006

Samuelsen JT, Schwarze PE, Huitfeldt HS, Thrane EV, Låg M, Refsnes M, Skarpen E, Becher R (2006)
Regulation of rat alveolar type 2 cell proliferation in vitro involves type II cAMP-dependent protein kinase
Am J Physiol Lung Cell Mol Physiol, 292 (1), L232-9
DOI 10.1152/ajplung.00049.2006, PubMed 16980378

Publications 2005

Rosseland CM, Wierød L, Oksvold MP, Werner H, Ostvold AC, Thoresen GH, Paulsen RE, Huitfeldt HS, Skarpen E (2005)
Cytoplasmic retention of peroxide-activated ERK provides survival in primary cultures of rat hepatocytes
Hepatology, 42 (1), 200-7
DOI 10.1002/hep.20762, PubMed 15962331

Skarpen E, Oksvold MP, Grøsvik H, Widnes C, Huitfeldt HS (2005)
Altered regulation of EGF receptor signaling following a partial hepatectomy
J Cell Physiol, 202 (3), 707-16
DOI 10.1002/jcp.20171, PubMed 15389569

Publications 2002

Oksvold MP, Huitfeldt HS, Østvold AC, Skarpen E (2002)
UV induces tyrosine kinase-independent internalisation and endosome arrest of the EGF receptor
J Cell Sci, 115 (Pt 4), 793-803
PubMed 11865035

Oksvold MP, Skarpen E, Widerberg J, Huitfeldt HS (2002)
Fluorescent histochemical techniques for analysis of intracellular signaling
J Histochem Cytochem, 50 (3), 289-303
DOI 10.1177/002215540205000301, PubMed 11850432

Publications 2001

Oksvold MP, Skarpen E, Wierød L, Paulsen RE, Huitfeldt HS (2001)
Re-localization of activated EGF receptor and its signal transducers to multivesicular compartments downstream of early endosomes in response to EGF
Eur J Cell Biol, 80 (4), 285-94
DOI 10.1078/0171-9335-00160, PubMed 11370743

Publications 2000

Lindeman B, Skarpen E, Oksvold MP, Huitfeldt HS (2000)
The carcinogen 2-acetylaminofluorene inhibits activation and nuclear accumulation of cyclin-dependent kinase 2 in growth-induced rat liver
Mol Carcinog, 27 (3), 190-9
DOI 10.1002/(SICI)1098-2744(200003)27:3<190::AID-MC6>3.3.CO;2-E, PubMed 10708481

Låg M, Skarpen E, van Rozendaal BA, Haagsman HP, Huitfeldt HS, Thrane EV, Schwarze PE (2000)
Cell-specific expression of CCAAT/enhancer-binding protein delta (C/EBP delta) in epithelial lung cells
Exp Lung Res, 26 (5), 383-99
DOI 10.1080/019021400408326, PubMed 10914335

Oksvold MP, Skarpen E, Lindeman B, Roos N, Huitfeldt HS (2000)
Immunocytochemical localization of Shc and activated EGF receptor in early endosomes after EGF stimulation of HeLa cells
J Histochem Cytochem, 48 (1), 21-33
DOI 10.1177/002215540004800103, PubMed 10653583

Skarpen E, Lindeman B, Thoresen GH, Guren TK, Oksvold MP, Christoffersen T, Huitfeldt HS (2000)
Impaired nuclear accumulation and shortened phosphorylation of ERK after growth factor stimulation in cultured hepatocytes from rats exposed to 2-acetylaminofluorene
Mol Carcinog, 28 (2), 84-96
DOI 10.1002/1098-2744(200006)28:2<84::aid-mc4>3.0.co;2-x, PubMed 10900465

Publications 1999

Huitfeldt HS, Lindeman B, Skarpen E, Oksvold MP, Wierød L (1999)
[Why is dioxin harmful?]
Tidsskr Nor Laegeforen, 119 (29), 4323-8
PubMed 10667131

Lindeman B, Skarpen E, Thoresen GH, Christoffersen T, Wierød L, Madshus IH, Huitfeldt HS (1999)
Alteration of G1 cell-cycle protein expression and induction of p53 but not p21/waf1 by the DNA-modifying carcinogen 2-acetylaminofluorene in growth-stimulated hepatocytes in vitro
Mol Carcinog, 24 (1), 36-46
DOI 10.1002/(sici)1098-2744(199901)24:1<36::aid-mc6>3.0.co;2-i, PubMed 10029409

Publications 1998

Lindeman B, Skarpen E, Huitfeldt HS (1998)
Stress protein expression in rat liver during tumour promotion: induction of heat-shock protein 27 in hepatocytes exposed to 2-acetylaminofluorene
Carcinogenesis, 19 (9), 1559-63
DOI 10.1093/carcin/19.9.1559, PubMed 9771925

Skarpen E, Johannessen LE, Bjerk K, Fasteng H, Guren TK, Lindeman B, Thoresen GH, Christoffersen T, Stang E, Huitfeldt HS, Madshus IH (1998)
Endocytosed epidermal growth factor (EGF) receptors contribute to the EGF-mediated growth arrest in A431 cells by inducing a sustained increase in p21/CIP1
Exp Cell Res, 243 (1), 161-72
DOI 10.1006/excr.1998.4127, PubMed 9716460

Skarpen E, Thoresen GH, Taskén K, Samuelsen JT, Jahnsen T, Schwarze PE, Huitfeldt HS (1998)
Localization of cAMP-dependent signal transducers in early rat liver carcinogenesis
Histochem Cell Biol, 109 (3), 203-9
DOI 10.1007/s004180050219, PubMed 9541468

Publications 1996

Huitfeldt HS, Skarpen E, Lindeman B, Becher R, Thrane EV, Schwarze PE (1996)
Differential distribution of Met and epidermal growth factor receptor in normal and carcinogen-treated rat liver
J Histochem Cytochem, 44 (3), 227-33
DOI 10.1177/44.3.8648082, PubMed 8648082

Publications 1995

Huitfeldt HS, Skarpen E, Lindeman B, van Gijssel HE, Nyhus PA, Leyshon-Sørland K (1995)
Early cellular alterations in chemical liver carcinogenesis as addressed by immunohistochemical and molecular biological techniques
Arch Toxicol Suppl, 17, 547-53
DOI 10.1007/978-3-642-79451-3_48, PubMed 7786191

HUITFELDT HS, SKARPEN E, LINDEMAN B, VANGIJSSEL HE, NYHUS PA, LEYSHONSORLAND K (1995)
EARLY CELLULAR ALTERATIONS IN CHEMICAL LIVER CARCINOGENESIS AS ASSESSED BY IMMUNOHISTOCHEMICAL AND MOLECULAR BIOLOGICAL TECHNIQUES
ARCH TOX S (17), 547-553

Skarpen E, Lindeman B, Thoresen GH, Låg M, Christoffersen T, Huitfeldt HS (1995)
CCAAT/enhancer-binding protein (C/EBP) immunoreactivity during rat liver carcinogenesis
Histochem Cell Biol, 104 (4), 287-94
DOI 10.1007/bf01464324, PubMed 8548562

Publications 1994

Huitfeldt HS, Heyden A, Skarpen E, Thrane EV, Schwarze PE (1994)
Gene activation studied by immunological methods
Environ Health Perspect, 102 Suppl 6, 205-7
DOI 10.1289/ehp.94102s6205, PubMed 7889849

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