❭ Research by medical division ❭ Cancer Medicine ❭ Institute for Cancer Research ❭ Dept. of Molecular Cell Biology ❭ Jorrit Enserink's group ❭ Protocols ❭

alpha-factor synchronization (arrest-release)

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TCA Precipitation for S. cerevisiae

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Random sporulation

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Mini prep

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Induction test of a recombinant protein

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His-FLAG-SUMO targets pull down in denaturing conditions

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GST-fusion protein purification

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Flow Cytometry in yeast (Sytox green)

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Flow cytometry in yeast (Propidium Iodide)

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Coomassie Staining and Destaining

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Immunoprecipitation

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Preparing yeast protein extracts for IP

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ChIP-MS

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ChIP in Yeast (for ~ 20 ChIPs)

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Analysis of RNA capping in vivo (RNA IP)

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BaF3 cell transfection protocol

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CellTiter-Glo Assay

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Protein isolation using TCA precipitation

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Recent publications

Ramos-Alonso L, Holland P, Le Gras S, Zhao X, Jost B, Bjørås M, Barral Y, Enserink JM, Chymkowitch P (2023)
Mitotic chromosome condensation resets chromatin to safeguard transcriptional homeostasis during interphase
Proc Natl Acad Sci U S A, 120 (4), e2210593120
DOI 10.1073/pnas.2210593120, PubMed 36656860

Tislevoll BS, Hellesøy M, Fagerholt OHE, Gullaksen SE, Srivastava A, Birkeland E, Kleftogiannis D, Ayuda-Durán P, Piechaczyk L, Tadele DS, Skavland J, Panagiotis B, Hovland R, Andresen V, Seternes OM, Tvedt THA, Aghaeepour N, Gavasso S, Porkka K, Jonassen I, Fløisand Y, Enserink J, Blaser N, Gjertsen BT (2023)
Early response evaluation by single cell signaling profiling in acute myeloid leukemia
Nat Commun, 14 (1), 115
DOI 10.1038/s41467-022-35624-4, PubMed 36611026

Hanes R, Ayuda-Durán P, Rønneberg L, Nakken S, Hovig E, Zucknick M, Enserink JM (2023)
screenwerk: a modular tool for the design and analysis of drug combination screens
Bioinformatics, 39 (1)
DOI 10.1093/bioinformatics/btac840, PubMed 36573326



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Get in touch

Department of Molecular Cell Biology, Institute for Cancer Research
The Norwegian Radium Hospital, Montebello, N-0379 Oslo, Norway
Telephone +47 22 78 19 82 (Enserink), +47 22 78 12 69 (Dept. Secretary)
Email: jorrit.enserink@rr-research.no

Oslo University Hospital is a part of Southern and Eastern Norway Regional Health Authority.

Webmaster for research pages: Trond Olav Berg

Email: trond.olav.berg@rr-research.no

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