alpha-factor synchronization (arrest-release)

TCA Precipitation for S. cerevisiae

Random sporulation

Induction test of a recombinant protein

His-FLAG-SUMO targets pull down in denaturing conditions

GST-fusion protein purification

Flow Cytometry in yeast (Sytox green)

Flow cytometry in yeast (Propidium Iodide)

Coomassie Staining and Destaining

Immunoprecipitation

Preparing yeast protein extracts for IP

ChIP in Yeast (for ~ 20 ChIPs)

Analysis of RNA capping in vivo (RNA IP)

BaF3 cell transfection protocol

CellTiter-Glo Assay

Protein isolation using TCA precipitation