alpha-factor synchronization (arrest-release)

 

TCA Precipitation for S. cerevisiae

 

Random sporulation

 

Induction test of a recombinant protein

 

His-FLAG-SUMO targets pull down in denaturing conditions

 

GST-fusion protein purification

 

Flow Cytometry in yeast (Sytox green)

 

Flow cytometry in yeast (Propidium Iodide)

 

Coomassie Staining and Destaining

 

Immunoprecipitation

 

Preparing yeast protein extracts for IP

 

ChIP in Yeast (for ~ 20 ChIPs)

 

Analysis of RNA capping in vivo (RNA IP)

 

BaF3 cell transfection protocol

 

CellTiter-Glo Assay

 

Protein isolation using TCA precipitation