alpha-factor synchronization (arrest-release)


TCA Precipitation for S. cerevisiae


Random sporulation


Induction test of a recombinant protein


His-FLAG-SUMO targets pull down in denaturing conditions


GST-fusion protein purification


Flow Cytometry in yeast (Sytox green)


Flow cytometry in yeast (Propidium Iodide)


Coomassie Staining and Destaining




Preparing yeast protein extracts for IP


ChIP in Yeast (for ~ 20 ChIPs)


Analysis of RNA capping in vivo (RNA IP)


BaF3 cell transfection protocol


CellTiter-Glo Assay


Protein isolation using TCA precipitation