- Characterization of novel gene regulatory mechanisms, with a focus on epigenetic and epitranscriptomic processes, required for sustenance and proliferation of GBM
- Identification of novel epigenetic and epitranscriptomic regulators required for GBM
- Biological validation and biochemical characterization of the identified hits
Aside from standard molecular biology tools, we apply CRISPR/Cas9 technology to carry out loss-of-function experiments and to edit endogenous loci in the genome. Furthermore, we use a variety of high-throughput sequencing to study gene regulation including RNA-seq, meRIP-seq, ChIP-seq, CLIP-seq and SLAM-seq.