Service and pricing

Gene expression 

mRNA expression
Affymetrix (via ThermoFisher) offer a range of high density arrays for human, mouse and rat, in addition to numerous other species. More information to be found on their web-page (https://www.thermofisher.com/no/en/home/life-science/microarray-analysis.html).
Using standard Clariom S arrays  with standard input totalRNA protocol: 50-200 ng. Preferred 150 ng. 

Array: https://www.thermofisher.com/order/catalog/product/902916?SID=srch-srp-902916
- Price: NOK 3600 per sample.
Low input totalRNA protocol: >500 pg.
- Price: NOK 4100 per sample.

miRNA expression
miRNA arrays are offered from Affymetrix (https://www.affymetrix.com/support/technical/datasheets/miRNA_4-0_and_4-1_datasheet.pdf).
Price: NOK 3200 for one sample. Input total RNA: 100-500 ng totalRNA

Alternative splicing
Affymetrix offer high resolution microarray for gene expression profiling of all transcript isoforms using Clariom D arrays.
Standard input totalRNA protocol: 50-200 ng. Preferred 150 ng.

Array: https://www.thermofisher.com/order/catalog/product/902914?SID=srch-srp-902914
- Price: NOK 5500 per sample.
Low input totalRNA protocol: >500 pg.
- Price: NOK 6000 per sample.

Bioinformatics 

The Core Facility provides free experimental design consultation. Based on the user’s needs, we provide different levels of bioinformatics analysis, i.e. preprocessing of data, normalization, statistical analysis, annotation, gene ontology and pathway analysis. This service is charged a per hour fee.

RNA purification 

The Core Facility provides RNA purification service. This service is charged a per hour fee. Expenses for purification kits and laboratory disposables are additional.

Verification of differentially expressed genes 

The Core Facility provides a service for verification of differentially expressed genes by qRT-PCR using a ViiA 7 Real-Time PCR System and TaqMan Gene Expression Assays. This service is charged a per hour fee. Expenses for TaqMan Gene Expression Assays and laboratory disposables etc. are additional.

RNA purification from FFPE material

We have successfully purified RNA from formalin-fixed, paraffin-embedded (FFPE) material and applied the RNA in microarray analysis using the large array Clariom D:

https://pubmed.ncbi.nlm.nih.gov/36034711/

https://pubmed.ncbi.nlm.nih.gov/33387485/

Contact Berit Brusletto for prices and further information.