Current RNA interference (RNAi) technologies often yield confusing results in functional studies and siRNA screens due to the unwanted effects such as the activation of innate immunity and silencing off-target genes. We have alleviated these problems by developing innovative strategies that separate RNAi from immune activation and off-target effects. Depending of the design, we also demonstrated that the function of dendritic cells can be modulated by RNAi resulting in the generation of "super" DCs capable of activating T cells in vitro and in vivo. Thus, immune tolerance against tumour antigens can be broken by altering DC function and vaccine design. I will present some of the data mentioned-above and also provide a teaching overview for the beginner and the expert.
Home page of Mouldy Sioud's group - Molecular Medicine
Institute seminars, spring 2009