Crystal structure of GLUE complexed to ubiquitin published in Nature Structural & Molecular Biology
T. Slagsvold
Thomas Slagsvold, a postdoc in Harald Stenmarks lab has previously identified a novel ubiquitin- and phosphoinositide binding domain, called GLUE. This identification was in collaboration with Rein Aasland at the University of Bergen and Soichi Wakatukis group at the Structural Biology Research Center in Ibaraki, Japan. In a continued collaboration with Wakatsukis group the crystal structure of the GLUE domain complexed to ubiquitin has now been solved. The structure is published in a recent issue of the prestigious journal "Nature Structural & Molecular Biology" (impact factor 12,19).
The GLUE domain is found in the endosomal protein Eap45, which is involved in sorting of ubiquitinated membrane proteins from endosomes to lysosomes. An article from the Stenmark group - entitled "Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain", with Slagsvold as first writer, was selected "JBC Paper of the week" last year - click here to read more.
The structure shows how the GLUE domain binds to the hydrophobic patch of ubiquitin and reveals that the ubiquitin- and phosphoinositide-binding sites of the GLUE domains are separate.
(click to enlarge)
Recent publications
From major journals, first or last author from the Institute for Cancer Research
Ellingsen EB, Aamdal E, Guren T, Lilleby W, Brunsvig PF, Mangsbo SM, Aamdal S, Hovig E, Mensali N, Gaudernack G, Inderberg EM(2022) Durable and dynamic hTERT immune responses following vaccination with the long-peptide cancer vaccine UV1: long-term follow-up of three phase I clinical trials J Immunother Cancer, 10(5) DOI 10.1136/jitc-2021-004345, PubMed 35613827
Skotland T, Iversen TG, Llorente A, Sandvig K(2022) Biodistribution, pharmacokinetics and excretion studies of intravenously injected nanoparticles and extracellular vesicles: Possibilities and challenges Adv Drug Deliv Rev, 186, 114326(in press) DOI 10.1016/j.addr.2022.114326, PubMed 35588953
Ianevski A, Giri AK, Aittokallio T(2022) SynergyFinder 3.0: an interactive analysis and consensus interpretation of multi-drug synergies across multiple samples Nucleic Acids Res(in press) DOI 10.1093/nar/gkac382, PubMed 35580060
Smirnov P, Smith I, Safikhani Z, Ba-Alawi W, Khodakarami F, Lin E, Yu Y, Martin S, Ortmann J, Aittokallio T, Hafner M, Haibe-Kains B(2022) Evaluation of statistical approaches for association testing in noisy drug screening data BMC Bioinformatics, 23(1), 188 DOI 10.1186/s12859-022-04693-z, PubMed 35585485
Ianevski A, Giri AK, Aittokallio T(2022) SynergyFinder 3.0: an interactive analysis and consensus interpretation of multi-drug synergies across multiple samples Nucleic Acids Res(in press) DOI 10.1093/nar/gkac382, PubMed 35580060
Skotland T, Iversen TG, Llorente A, Sandvig K(2022) Biodistribution, pharmacokinetics and excretion studies of intravenously injected nanoparticles and extracellular vesicles: Possibilities and challenges Adv Drug Deliv Rev, 186, 114326(in press) DOI 10.1016/j.addr.2022.114326, PubMed 35588953
