Some relevant publications
Berg,K.,Bommer,J.,Moan,J. (1989) Evaluation of sulfonated aluminum phthalocyanines for use in photochemotherapy. Cellular uptake studies.
Cancer Lett. 44,7-15.
Berg,K.,Bommer,J.,Moan,J. (1989) Evaluation of sulfonated aluminum phthalocyanines for use in photochemotherapy. A study on the relative efficiencies of photoinactivation.
Photochem.Photobiol. 49,587-594.
Moan,J.,Berg,K.,Kvam,E.,Evensen,J.F.,Malik,Z.,Ruck,A., Schneckenburger,H. (1989) Intracellular localication of photosensitizers. In: Photosensitizing Compounds: Their Chemistry, Biology and Clinical Use. (Eds. G.Bock and S.Harnett), Wiley, Chichester (Ciba Foundation Symposium 146), pp.95-111.
Berg,K.,Western,A.,Bommer,J. and Moan,J. (1990) Intracellular localization of sulfonated meso-tetraphenylporphines in a human carcinoma cell line.
Photochem.Photobiol. 52:481-487.
Berg,K.,Western,A.,Bommer,J. and Moan,J. (1990) Cellular uptake and relative efficiency in cell inactivation by photoactivated sulfonated meso-tetraphenylporphines.
Photochem.Photobiol. 52:775-781.
Berg,K.,Madslien,K.,Bommer J.C.,Oftebro,R.,Winkelman,J.C. and Moan,J. (1991)
Light induced relocalization of sulfonated meso-tetraphenylporphines in NHIK 3025 cells and effects of dose fractionation.
Photochem.Photobiol. 53:203-210.
Moan,J. and Berg,K. (1991) The photodegradation of porphyrins in cells can be used to estimate the lifetime of singlet oxygen.
Photochem.Photobiol. 53:549-553.
Moan,J.,Berg,K. and Western,A. (1992) Action spectra of phthalocyanines with respect to photosensitization of cells.
Photochem.Photobiol. 56:171-175.
Berg,K.,Madslien,K. and Moan,J. (1992) Retention of phototoxicity of tetra(sulfonatophenyl)porphine in cultivated human cells. The effect of fractionation of light.
Photochem.Photobiol. 56:177-183.
Berg,K.,Prydz,K. and Moan,J. (1993) Photochemical treatment with the lysosomally localized dye tetra(4-sulfonatophenyl)porphine results in lysosomal release of the dye but not of b-N-acetyl-D-glucosaminidase activity.
Biochim.Biophys.Acta. 1158: 300-306.
Moan,J. Berg,K., Anholt,H. and Madslien,K. (1994) Sulfonated aluminium phthalocyanines as sensitizers for photochemotherapy. Effects of small light doses on localization, dye fluorescence and photosensitivity in V79 cells.
Int.J.Cancer 58: 865-870.
Berg,K. and Moan,J. (1994) Lysosomes as photochemical targets.
Int.J.Cancer. 59: 814-822.
Berg,K. and Moan,J. (1995) The influence of the cysteine protease inhibitor E64 on photobiological effects of tetrasulfonated phenylporphines.
Cancer Lett. 88:227-236.
Berg,K. (1996) Mechanism of cell damage in Photodynamic therapy. In: The Fundamental Bases of Phototherapy (Eds. A.Young ,H.Honigsmann and G.Jori) OEMF, Milano, pp.181-207.
Strømhaug,P.E., Berg,T.O., Berg,K. and Seglen,P.O. (1997) A novel method to study autophagy: Destruction of hepatocytic lysosomes, but not autophagosomes, by the photosensitizing porphyrin tetra(4-sulfonatophenyl)porhine (TPPS4).
Biochem.J. 321:217-225
Berg,K., and Moan,J. (1997) Lysosomes and microtubules as targets in photochemotherapy of cancer.
Photochem.Photobiol. 65: 403-409.
Brustad,T., Voss,T., Selbo,P.K., Gärtner,K., Holmøy,T., Nesland,J.M. and Berg,K.
(1997) Vital Microscopy and its use to Elucidate Photodynamic Effects.
Internet J. of Science. Biological Chemistry Vol.3. http://www.photobiology.com/v1/selbo/vitalmic.htm
Selbo,P.K.,Brustad,T.,Voss,T.,Moan,J. and Berg,K. "In situ localization of a photosensitizer
as evaluated by vital microscopy" in Photochemotherapy: Photodynamic Therapy and Other Modalities III, Kristian Berg, Benjamin Ehrenberg, Zvi Malik, Johan Moan, Editors, Proc.SPIE 3191, 25-32 (1997).
L.Prasmickaite, A.Høgset G,Maelandsmo, K.Berg, Goodchild,J, Perkins,T., Fodstad,Ø., and E.Hovig. (1998) Intracellular metabolism of a 2'-O-methyl stabilized ribozyme after uptake by DOTAP-transfection or as free ribozyme. A study by capillary electrophoresis.
Nucleic Acids Research 26: 4241-4248
Berg,K., Selbo,P.K , Prasmickaite,L., Tjelle,T.E., Kjølsrud,S., Rodal, G.H., Anholt,H., Sandvig,K., Moan,J., Gaudernack,G., Fodstad,Ø., Rodal,S.K. and Høgset,A. (1999) Photochemical internalization. A novel technology for site-specific delivery of macromolecules into cytosol.
Cancer Res. 59: 1180-1183.
Høgset,A., Prasmickaite,L., Tjelle,T.E. and Berg,K (2000) Photochemical transfection, a new technology for light-induced, site-directed gene delivery.
Human Gene Therapy 11: 869-880.
Selbo,P.K., Sandvig,K., Kirveliene,V. and Berg,K. (2000) Release of gelonin from endosomes and lysosomes to cytosol by photochemical internalization.
Biochim.Biophys.Acta 1475: 307-313.
Selbo, P.K., Sivam,G., Fodstad,Ø., Sandvig.K. and Berg,K. (2000) Photochemical internalisation increases the cytotoxic effect of the immunotoxin MOC31-gelonin.
Int. J.Cancer 87: 853-859.
Prasmickaite,L.,Høgset,A.,Tjelle,T.E., Sandvig,K. Berg,K. (2000) The role of endosomes in gene transfection mediated by photochemical internalisation.
J.Gene Medicine 2: 477-488.
Pål Kristian Selbo, Gowsala Sivam, Øystein Fodstad, Kirsten Sandvig and Kristian Berg. (2001) In vivo documentation of photochemical internalization, a novel approach for site specific cancer therapy.
Int.J.Cancer 92: 761-766.
Prasmickaite, L., Høgset, A. and Berg, K. (2001) Effects of different photosensitizing compounds on light induced transfection.
Photochem.Photobiol. 73: 388-395.
Selbo,P.K. Kaalhus, O. Sivam,G. and Berg,K. (2001) 5-aminolevulinic acid based photochemical internalization of the immunotoxin MOC31-gelonin generates synergistic cytotoxic effects in vitro.
Photochem.Photobiol. 74: 303-310.
Prasmickaite,L. Høgset A. and Berg,K. (2002) Photochemical transfection. Light-induced, site-directed gene delivery. Methods Mol Med.69:123-35.
Prasmickaite, L., Høgset, A., Selbo, P.K., Engesæter, B.Ø., Hellum, M. and Berg, K. (2002). Photochemical disruption of endocytic vesicles before delivery of drugs: a new strategy for cancer therapy.
Br.J.Cancer 86: 652-657.
Selbo, P.K., Høgset, A., Prasmickaite,L. and Berg, K. (2002) Photochemical internalisation: a novel drug delivery system.
Tumor Biology 23: 103-112.
Høgset A, Engesæter B.Ø, Prasmickaite L, Berg K, Fodstad Ø, Maelandsmo GM.
(2002) Light-induced adenovirus gene transfer, potential site-directed gene delivery technology for in vivo gene therapy.
Cancer Gene Ther. 9: 365-371
Prasmickaite, L., Høgset, A. and Berg, K. (2002). The role of cell cycle on the efficiency of photochemical gene transfection.
Biochim. Biophys. Acta, 1570, 210-218.
Høgset, A., Prasmickaite, L., Hellum, M., Engesæter, B.Ø., Olsen, V.M., Tjelle, T.E., Wheeler, C.J. and Berg, K. (2002). Photochemical transfection, a technology for efficient light directed gene delivery.in "Synthetic DNA Delivery Systems" (Saltzman, W.M. and Luo, D., eds). Landes Bioscience, Austin and Kluwer Academic Publishers, Dordrecht.
Høgset, A., Prasmickaite, L., Hellum, M., Engesæter, B.Ø., Olsen, V.M., Tjelle, T.E., Wheeler, C.J. and Berg, K. (2002). Photochemical transfection, a technology for efficient light directed gene delivery.
Somat. Cell Molec. Genet. 27:97-113.
Berg, K., Prasmickaite,L., Selbo,P.K., Hellum,M., Bonsted,A. and Høgset,A. (2003) Photochemical internalisation (PCI) - a novel technology for release of macromolecules from endocytic vesicles
Oftamologia 56, pp. 67-71.
Marit Hellum, Anders Høgset, Birgit Ø. Engesæter, Lina Prasmickaite, Stokke,L., Carl J Wheeler, Kristian Berg (2003) Photochemically enhanced gene delivery with cationic lipid/DNA complexes - time course of intracellular events during transfection.
Photochem.Photobiol.Sci.2:407-11.
Høgset, A., Prasmickaite,L., Engesæter,B.Ø., Hellum,M., Selbo,P.K., Olsen,V.M. Mælandsmo, G.M.and Berg,K. (2003) Light directed gene transfer by photochemical internalisation. Curr. Gene Ther. 3, 89-112.
Marco Folini, Kristian Berg, Enrico Millo, Raffaella Villa, Maria Grazia Dandone, Lina Prasmickaite and Nadia Zaffaroni (2003) Photochemical internalization of a peptide nucleic acid targeting the catalytic subunit of human telomerase
Cancer Res. 63: 3490-3494.
Dietze,A., Bonsted,A. Høgset, A., Berg,K. (2003) Photochemical internalisaton (PCI) enhances the cytotoxic effect of the protein toxin gelonin and transgene expression in sarcoma cells
Photochem.Photobiol. 78(3):283-9.
In press:
Høgset, A., Prasmickaite, L., Selbo, P.K., Hellum, M., Engesæter, B.Ø. and Berg, K.
Photochemical internalisation in drug and gene delivery.
Adv. Drug Deliv. Rev. Invited review.
Bonsted, A., Engesæter,B.Ø. Høgset, A., Mælandsmo, G.M.. Prasmickaite, L., Kaalhus, O. and Berg, K. Complexes of adenovirus with polycations increase the efficiency of photochemicvally mediated transduction
Gene therapy
Cancer Lett. 44,7-15.
Berg,K.,Bommer,J.,Moan,J. (1989) Evaluation of sulfonated aluminum phthalocyanines for use in photochemotherapy. A study on the relative efficiencies of photoinactivation.
Photochem.Photobiol. 49,587-594.
Moan,J.,Berg,K.,Kvam,E.,Evensen,J.F.,Malik,Z.,Ruck,A., Schneckenburger,H. (1989) Intracellular localication of photosensitizers. In: Photosensitizing Compounds: Their Chemistry, Biology and Clinical Use. (Eds. G.Bock and S.Harnett), Wiley, Chichester (Ciba Foundation Symposium 146), pp.95-111.
Berg,K.,Western,A.,Bommer,J. and Moan,J. (1990) Intracellular localization of sulfonated meso-tetraphenylporphines in a human carcinoma cell line.
Photochem.Photobiol. 52:481-487.
Berg,K.,Western,A.,Bommer,J. and Moan,J. (1990) Cellular uptake and relative efficiency in cell inactivation by photoactivated sulfonated meso-tetraphenylporphines.
Photochem.Photobiol. 52:775-781.
Berg,K.,Madslien,K.,Bommer J.C.,Oftebro,R.,Winkelman,J.C. and Moan,J. (1991)
Light induced relocalization of sulfonated meso-tetraphenylporphines in NHIK 3025 cells and effects of dose fractionation.
Photochem.Photobiol. 53:203-210.
Moan,J. and Berg,K. (1991) The photodegradation of porphyrins in cells can be used to estimate the lifetime of singlet oxygen.
Photochem.Photobiol. 53:549-553.
Moan,J.,Berg,K. and Western,A. (1992) Action spectra of phthalocyanines with respect to photosensitization of cells.
Photochem.Photobiol. 56:171-175.
Berg,K.,Madslien,K. and Moan,J. (1992) Retention of phototoxicity of tetra(sulfonatophenyl)porphine in cultivated human cells. The effect of fractionation of light.
Photochem.Photobiol. 56:177-183.
Berg,K.,Prydz,K. and Moan,J. (1993) Photochemical treatment with the lysosomally localized dye tetra(4-sulfonatophenyl)porphine results in lysosomal release of the dye but not of b-N-acetyl-D-glucosaminidase activity.
Biochim.Biophys.Acta. 1158: 300-306.
Moan,J. Berg,K., Anholt,H. and Madslien,K. (1994) Sulfonated aluminium phthalocyanines as sensitizers for photochemotherapy. Effects of small light doses on localization, dye fluorescence and photosensitivity in V79 cells.
Int.J.Cancer 58: 865-870.
Berg,K. and Moan,J. (1994) Lysosomes as photochemical targets.
Int.J.Cancer. 59: 814-822.
Berg,K. and Moan,J. (1995) The influence of the cysteine protease inhibitor E64 on photobiological effects of tetrasulfonated phenylporphines.
Cancer Lett. 88:227-236.
Berg,K. (1996) Mechanism of cell damage in Photodynamic therapy. In: The Fundamental Bases of Phototherapy (Eds. A.Young ,H.Honigsmann and G.Jori) OEMF, Milano, pp.181-207.
Strømhaug,P.E., Berg,T.O., Berg,K. and Seglen,P.O. (1997) A novel method to study autophagy: Destruction of hepatocytic lysosomes, but not autophagosomes, by the photosensitizing porphyrin tetra(4-sulfonatophenyl)porhine (TPPS4).
Biochem.J. 321:217-225
Berg,K., and Moan,J. (1997) Lysosomes and microtubules as targets in photochemotherapy of cancer.
Photochem.Photobiol. 65: 403-409.
Brustad,T., Voss,T., Selbo,P.K., Gärtner,K., Holmøy,T., Nesland,J.M. and Berg,K.
(1997) Vital Microscopy and its use to Elucidate Photodynamic Effects.
Internet J. of Science. Biological Chemistry Vol.3. http://www.photobiology.com/v1/selbo/vitalmic.htm
Selbo,P.K.,Brustad,T.,Voss,T.,Moan,J. and Berg,K. "In situ localization of a photosensitizer
as evaluated by vital microscopy" in Photochemotherapy: Photodynamic Therapy and Other Modalities III, Kristian Berg, Benjamin Ehrenberg, Zvi Malik, Johan Moan, Editors, Proc.SPIE 3191, 25-32 (1997).
L.Prasmickaite, A.Høgset G,Maelandsmo, K.Berg, Goodchild,J, Perkins,T., Fodstad,Ø., and E.Hovig. (1998) Intracellular metabolism of a 2'-O-methyl stabilized ribozyme after uptake by DOTAP-transfection or as free ribozyme. A study by capillary electrophoresis.
Nucleic Acids Research 26: 4241-4248
Berg,K., Selbo,P.K , Prasmickaite,L., Tjelle,T.E., Kjølsrud,S., Rodal, G.H., Anholt,H., Sandvig,K., Moan,J., Gaudernack,G., Fodstad,Ø., Rodal,S.K. and Høgset,A. (1999) Photochemical internalization. A novel technology for site-specific delivery of macromolecules into cytosol.
Cancer Res. 59: 1180-1183.
Høgset,A., Prasmickaite,L., Tjelle,T.E. and Berg,K (2000) Photochemical transfection, a new technology for light-induced, site-directed gene delivery.
Human Gene Therapy 11: 869-880.
Selbo,P.K., Sandvig,K., Kirveliene,V. and Berg,K. (2000) Release of gelonin from endosomes and lysosomes to cytosol by photochemical internalization.
Biochim.Biophys.Acta 1475: 307-313.
Selbo, P.K., Sivam,G., Fodstad,Ø., Sandvig.K. and Berg,K. (2000) Photochemical internalisation increases the cytotoxic effect of the immunotoxin MOC31-gelonin.
Int. J.Cancer 87: 853-859.
Prasmickaite,L.,Høgset,A.,Tjelle,T.E., Sandvig,K. Berg,K. (2000) The role of endosomes in gene transfection mediated by photochemical internalisation.
J.Gene Medicine 2: 477-488.
Pål Kristian Selbo, Gowsala Sivam, Øystein Fodstad, Kirsten Sandvig and Kristian Berg. (2001) In vivo documentation of photochemical internalization, a novel approach for site specific cancer therapy.
Int.J.Cancer 92: 761-766.
Prasmickaite, L., Høgset, A. and Berg, K. (2001) Effects of different photosensitizing compounds on light induced transfection.
Photochem.Photobiol. 73: 388-395.
Selbo,P.K. Kaalhus, O. Sivam,G. and Berg,K. (2001) 5-aminolevulinic acid based photochemical internalization of the immunotoxin MOC31-gelonin generates synergistic cytotoxic effects in vitro.
Photochem.Photobiol. 74: 303-310.
Prasmickaite,L. Høgset A. and Berg,K. (2002) Photochemical transfection. Light-induced, site-directed gene delivery. Methods Mol Med.69:123-35.
Prasmickaite, L., Høgset, A., Selbo, P.K., Engesæter, B.Ø., Hellum, M. and Berg, K. (2002). Photochemical disruption of endocytic vesicles before delivery of drugs: a new strategy for cancer therapy.
Br.J.Cancer 86: 652-657.
Selbo, P.K., Høgset, A., Prasmickaite,L. and Berg, K. (2002) Photochemical internalisation: a novel drug delivery system.
Tumor Biology 23: 103-112.
Høgset A, Engesæter B.Ø, Prasmickaite L, Berg K, Fodstad Ø, Maelandsmo GM.
(2002) Light-induced adenovirus gene transfer, potential site-directed gene delivery technology for in vivo gene therapy.
Cancer Gene Ther. 9: 365-371
Prasmickaite, L., Høgset, A. and Berg, K. (2002). The role of cell cycle on the efficiency of photochemical gene transfection.
Biochim. Biophys. Acta, 1570, 210-218.
Høgset, A., Prasmickaite, L., Hellum, M., Engesæter, B.Ø., Olsen, V.M., Tjelle, T.E., Wheeler, C.J. and Berg, K. (2002). Photochemical transfection, a technology for efficient light directed gene delivery.in "Synthetic DNA Delivery Systems" (Saltzman, W.M. and Luo, D., eds). Landes Bioscience, Austin and Kluwer Academic Publishers, Dordrecht.
Høgset, A., Prasmickaite, L., Hellum, M., Engesæter, B.Ø., Olsen, V.M., Tjelle, T.E., Wheeler, C.J. and Berg, K. (2002). Photochemical transfection, a technology for efficient light directed gene delivery.
Somat. Cell Molec. Genet. 27:97-113.
Berg, K., Prasmickaite,L., Selbo,P.K., Hellum,M., Bonsted,A. and Høgset,A. (2003) Photochemical internalisation (PCI) - a novel technology for release of macromolecules from endocytic vesicles
Oftamologia 56, pp. 67-71.
Marit Hellum, Anders Høgset, Birgit Ø. Engesæter, Lina Prasmickaite, Stokke,L., Carl J Wheeler, Kristian Berg (2003) Photochemically enhanced gene delivery with cationic lipid/DNA complexes - time course of intracellular events during transfection.
Photochem.Photobiol.Sci.2:407-11.
Høgset, A., Prasmickaite,L., Engesæter,B.Ø., Hellum,M., Selbo,P.K., Olsen,V.M. Mælandsmo, G.M.and Berg,K. (2003) Light directed gene transfer by photochemical internalisation. Curr. Gene Ther. 3, 89-112.
Marco Folini, Kristian Berg, Enrico Millo, Raffaella Villa, Maria Grazia Dandone, Lina Prasmickaite and Nadia Zaffaroni (2003) Photochemical internalization of a peptide nucleic acid targeting the catalytic subunit of human telomerase
Cancer Res. 63: 3490-3494.
Dietze,A., Bonsted,A. Høgset, A., Berg,K. (2003) Photochemical internalisaton (PCI) enhances the cytotoxic effect of the protein toxin gelonin and transgene expression in sarcoma cells
Photochem.Photobiol. 78(3):283-9.
In press:
Høgset, A., Prasmickaite, L., Selbo, P.K., Hellum, M., Engesæter, B.Ø. and Berg, K.
Photochemical internalisation in drug and gene delivery.
Adv. Drug Deliv. Rev. Invited review.
Bonsted, A., Engesæter,B.Ø. Høgset, A., Mælandsmo, G.M.. Prasmickaite, L., Kaalhus, O. and Berg, K. Complexes of adenovirus with polycations increase the efficiency of photochemicvally mediated transduction
Gene therapy