Spinning Disk Confocal Microscopy

Nikon ECLIPSE Ti2-E X-Light V3 spinning disk

The Nikon ECLIPSE Ti2-E inverted microscope, equipped with the CrestOptics X-Light V3 spinning disk confocal module, was installed at the core facility in late 2023 and is now available for user training and booking.

The Nikon CrestOptics spinning disk microscope is suitable for imaging live cells, fixed samples, thick tissue samples, as well as organoids, in either wide field or confocal mode. This instrument has a large field of view (25 x 25 mm) and uniform illumination (due to micro lenses) resulting in seamless stitching of large images. Penta-band and dual-band filters facilitate detection of emitted light from the most common fluorophores (DAPI, FITC, TRITC, Cy5, Cy7, CFP, YFP).

In addition, the dual camera setup and Z piezo device give fast imaging of multiple channels and z-stacks with nanometer precision.

Microscope features:

  • Motorized XY stage
  • Z piezo device
  • Stage top incubator chamber
  • CO2 and temperature control
  • Dual camera imaging (Back-illuminated sCMOS cameras, Photometrics Kinetix)
  • Lumencor Celesta multi-line laser (405, 446, 477, 518, 546, 638, 749 nm)
  • CrestOptics X-Light V3 spinning disk (50:400 µm deep imaging disk)
  • 4x 0.20 NA Plan Apochromat λD air objective
  • 10x 0.30 NA Plan Fluor DL air objective, Ph1
  • 20x 0.80 NA Plan Apochromat λD air objective
  • 40x 1.25 NA Plan Apochromat λS silicone oil objective
  • 60x 1.42 NA Plan Apochromat λD oil objective, DIC
  • Tube lens changer (1.5x extra magnification)
  • Anti-vibration table
  • NIS Elements AR software


Anna Lång, PhD

Telephone: +47 23013915

E-mail: a.u.lang@medisin.uio.no