Kirsten Skarstad
Position: Group leader, Professor
Phone: +47 22 78 19 82
Email:
 

Author network for Kirsten Skarstad by COREMINE medical


Publications 2017

Pedersen IB, Helgesen E, Flåtten I, Fossum-Raunehaug S, Skarstad K (2017)
SeqA structures behind Escherichia coli replication forks affect replication elongation and restart mechanisms
Nucleic Acids Res (in press)
PubMed 28407100

Publications 2016

Helgesen E, Fossum-Raunehaug S, Skarstad K (2016)
Lack of the H-NS Protein Results in Extended and Aberrantly Positioned DNA during Chromosome Replication and Segregation in Escherichia coli
J Bacteriol, 198 (8), 1305-16
PubMed 26858102

Publications 2015

Flåtten I, Fossum-Raunehaug S, Taipale R, Martinsen S, Skarstad K (2015)
The DnaA Protein Is Not the Limiting Factor for Initiation of Replication in Escherichia coli
PLoS Genet, 11 (6), e1005276
PubMed 26047361

Helgesen E, Fossum-Raunehaug S, Sætre F, Schink KO, Skarstad K (2015)
Dynamic Escherichia coli SeqA complexes organize the newly replicated DNA at a considerable distance from the replisome
Nucleic Acids Res, 43 (5), 2730-43
PubMed 25722374

Publications 2014

Fossum-Raunehaug S, Helgesen E, Stokke C, Skarstad K (2014)
Escherichia coli SeqA structures relocalize abruptly upon termination of origin sequestration during multifork DNA replication
PLoS One, 9 (10), e110575
PubMed 25333813

Morigen M, Flåtten I, Skarstad K (2014)
The Escherichia coli datA site promotes proper regulation of cell division
Microbiology, 160 (Pt 4), 703-10
PubMed 24574433

Odsbu I, Skarstad K (2014)
DNA compaction in the early part of the SOS response is dependent on RecN and RecA
Microbiology, 160 (Pt 5), 872-82
PubMed 24615185

Publications 2013

Flåtten I, Skarstad K (2013)
The Fis protein has a stimulating role in initiation of replication in Escherichia coli in vivo
PLoS One, 8 (12), e83562
PubMed 24358293

Skarstad K, Katayama T (2013)
Regulating DNA replication in bacteria
Cold Spring Harb Perspect Biol, 5 (4), a012922
PubMed 23471435

Weel-Sneve R, Kristiansen KI, Odsbu I, Dalhus B, Booth J, Rognes T, Skarstad K, Bjørås M (2013)
Single transmembrane peptide DinQ modulates membrane-dependent activities
PLoS Genet, 9 (2), e1003260
PubMed 23408903

Publications 2012

Fingland N, Flåtten I, Downey CD, Fossum-Raunehaug S, Skarstad K, Crooke E (2012)
Depletion of acidic phospholipids influences chromosomal replication in Escherichia coli
Microbiologyopen, 1 (4), 450-66
PubMed 23233230

Stokke C, Flåtten I, Skarstad K (2012)
An easy-to-use simulation program demonstrates variations in bacterial cell cycle parameters depending on medium and temperature
PLoS One, 7 (2), e30981
PubMed 22348034

Waldminghaus T, Weigel C, Skarstad K (2012)
Replication fork movement and methylation govern SeqA binding to the Escherichia coli chromosome
Nucleic Acids Res, 40 (12), 5465-76
PubMed 22373925

Publications 2011

Johnsen E, Wilson SR, Odsbu I, Krapp A, Malerod H, Skarstad K, Lundanes E (2011)
Hydrophilic interaction chromatography of nucleoside triphosphates with temperature as a separation parameter
J Chromatogr A, 1218 (35), 5981-6
PubMed 21315360

Publications 2010

Johnsen L, Flåtten I, Morigen, Dalhus B, Bjørås M, Waldminghaus T, Skarstad K (2010)
The G157C mutation in the Escherichia coli sliding clamp specifically affects initiation of replication
Mol Microbiol, 79 (2), 433-46
PubMed 21219462

Johnsen L, Weigel C, von Kries J, Møller M, Skarstad K (2010)
A novel DNA gyrase inhibitor rescues Escherichia coli dnaAcos mutant cells from lethal hyperinitiation
J Antimicrob Chemother, 65 (5), 924-30
PubMed 20228083

Stokke C, Waldminghaus T, Skarstad K (2010)
Replication patterns and organization of replication forks in Vibrio cholerae
Microbiology, 157 (Pt 3), 695-708
PubMed 21163839

Waldminghaus T, Skarstad K (2010)
ChIP on Chip: surprising results are often artifacts
BMC Genomics, 11, 414
PubMed 20602746

Publications 2009

Boeneman K, Fossum S, Yang Y, Fingland N, Skarstad K, Crooke E (2009)
Escherichia coli DnaA forms helical structures along the longitudinal cell axis distinct from MreB filaments
Mol Microbiol, 72 (3), 645-57
PubMed 19400775

Morigen, Odsbu I, Skarstad K (2009)
Growth rate dependent numbers of SeqA structures organize the multiple replication forks in rapidly growing Escherichia coli
Genes Cells, 14 (5), 643-57
PubMed 19371375

Odsbu I, Morigen, Skarstad K (2009)
A reduction in ribonucleotide reductase activity slows down the chromosome replication fork but does not change its localization
PLoS One, 4 (10), e7617
PubMed 19898675

Waldminghaus T, Skarstad K (2009)
The Escherichia coli SeqA protein
Plasmid, 61 (3), 141-50
PubMed 19254745

Publications 2008

Bach T, Morigen, Skarstad K (2008)
The initiator protein DnaA contributes to keeping new origins inactivated by promoting the presence of hemimethylated DNA
J Mol Biol, 384 (5), 1076-85
PubMed 18835566

Flåtten I, Morigen, Skarstad K (2008)
DnaA protein interacts with RNA polymerase and partially protects it from the effect of rifampicin
Mol Microbiol, 71 (4), 1018-30
PubMed 19170875

Fossum S, De Pascale G, Weigel C, Messer W, Donadio S, Skarstad K (2008)
A robust screen for novel antibiotics: specific knockout of the initiator of bacterial DNA replication
FEMS Microbiol Lett, 281 (2), 210-4
PubMed 18312360

Fuller RS, Bambara RA, Baker T, Funnell B, Wahle E, O'Donnell M, Kaiser D, Skarstad K, Konforti B, Maki S, Katayama T, Sekimizu K, Weiner JH, Davis RW, Rowen L, Goodman MF, Spudich J, Pfeffer S, Richardson CC, Polaczek P, Calendar R, Kolodner R, Griffith J, Stillman B, Modrich P et al. (2008)
A tribute to Arthur Kornberg 1918-2007
Nat. Struct. Mol. Biol., 15 (1), 2-17

Publications 2007

Fossum S, Crooke E, Skarstad K (2007)
Organization of sister origins and replisomes during multifork DNA replication in Escherichia coli
EMBO J, 26 (21), 4514-22
PubMed 17914458

Norris V, den Blaauwen T, Cabin-Flaman A, Doi RH, Harshey R, Janniere L, Jimenez-Sanchez A, Jin DJ, Levin PA, Mileykovskaya E, Minsky A, Saier M, Skarstad K (2007)
Functional taxonomy of bacterial hyperstructures
Microbiol Mol Biol Rev, 71 (1), 230-53
PubMed 17347523

Norris V, den Blaauwen T, Doi RH, Harshey RM, Janniere L, Jiménez-Sánchez A, Jin DJ, Levin PA, Mileykovskaya E, Minsky A, Misevic G, Ripoll C, Saier M, Skarstad K, Thellier M (2007)
Toward a hyperstructure taxonomy
Annu Rev Microbiol, 61, 309-29
PubMed 17896876

Publications 2006

Gon S, Camara JE, Klungsøyr HK, Crooke E, Skarstad K, Beckwith J (2006)
A novel regulatory mechanism couples deoxyribonucleotide synthesis and DNA replication in Escherichia coli
EMBO J, 25 (5), 1137-47
PubMed 16482221

Publications 2005

Bach T, Skarstad K (2005)
An oriC-like distribution of GATC sites mediates full sequestration of non-origin sequences in Escherichia coli
J Mol Biol, 350 (1), 7-11
PubMed 15922360

Morigen, Molina F, Skarstad K (2005)
Deletion of the datA site does not affect once-per-cell-cycle timing but induces rifampin-resistant replication
J Bacteriol, 187 (12), 3913-20
PubMed 15939703

Odsbu I, Klungsøyr HK, Fossum S, Skarstad K (2005)
Specific N-terminal interactions of the Escherichia coli SeqA protein are required to form multimers that restrain negative supercoils and form foci
Genes Cells, 10 (11), 1039-49
PubMed 16236133

Publications 2004

Bach T, Skarstad K (2004)
Re-replication from non-sequesterable origins generates three-nucleoid cells which divide asymmetrically
Mol Microbiol, 51 (6), 1589-600
PubMed 15009887

Klungsøyr HK, Skarstad K (2004)
Positive supercoiling is generated in the presence of Escherichia coli SeqA protein
Mol Microbiol, 54 (1), 123-31
PubMed 15458410

Molina F, Skarstad K (2004)
Replication fork and SeqA focus distributions in Escherichia coli suggest a replication hyperstructure dependent on nucleotide metabolism
Mol Microbiol, 52 (6), 1597-612
PubMed 15186411

Publications 2003

Bach T, Krekling MA, Skarstad K (2003)
Excess SeqA prolongs sequestration of oriC and delays nucleoid segregation and cell division
EMBO J, 22 (2), 315-23
PubMed 12514137

Camara JE, Skarstad K, Crooke E (2003)
Controlled initiation of chromosomal replication in Escherichia coli requires functional Hda protein
J Bacteriol, 185 (10), 3244-8
PubMed 12730188

Fossum S, Søreide S, Skarstad K (2003)
Lack of SeqA focus formation, specific DNA binding and proper protein multimerization in the Escherichia coli sequestration mutant seqA2
Mol Microbiol, 47 (3), 619-32
PubMed 12535065

Kang S, Han JS, Park JH, Skarstad K, Hwang DS (2003)
SeqA protein stimulates the relaxing and decatenating activities of topoisomerase IV
J Biol Chem, 278 (49), 48779-85
PubMed 14512422

Miller C, Ingmer H, Thomsen LE, Skarstad K, Cohen SN (2003)
DpiA binding to the replication origin of Escherichia coli plasmids and chromosomes destabilizes plasmid inheritance and induces the bacterial SOS response
J Bacteriol, 185 (20), 6025-31
PubMed 14526013

Morigen, Løbner-Olesen A, Skarstad K (2003)
Titration of the Escherichia coli DnaA protein to excess datA sites causes destabilization of replication forks, delayed replication initiation and delayed cell division
Mol Microbiol, 50 (1), 349-62
PubMed 14507385

Skarstad K, Boye E, Fanning E (2003)
Circles in the sand
EMBO Rep, 4 (7), 661-5
PubMed 12835752

Skarstad K, Løbner-Olesen A (2003)
Stable co-existence of separate replicons in Escherichia coli is dependent on once-per-cell-cycle initiation
EMBO J, 22 (1), 140-50
PubMed 12505992

Slominska M, Wahl A, Wegrzyn G, Skarstad K (2003)
Degradation of mutant initiator protein DnaA204 by proteases ClpP, ClpQ and Lon is prevented when DNA is SeqA-free
Biochem J, 370 (Pt 3), 867-71
PubMed 12479794

Publications 2001

Morigen, Boye E, Skarstad K, Løbner-Olesen A (2001)
Regulation of chromosomal replication by DnaA protein availability in Escherichia coli: effects of the datA region
Biochim Biophys Acta, 1521 (1-3), 73-80
PubMed 11690638

Skarstad K, Torheim N, Wold S, Lurz R, Messer W, Fossum S, Bach T (2001)
The Escherichia coli SeqA protein binds specifically to two sites in fully and hemimethylated oriC and has the capacity to inhibit DNA replication and affect chromosome topology
Biochimie, 83 (1), 49-51
PubMed 11254974

Slomińska M, Wegrzyn A, Konopa G, Skarstad K, Wegrzyn G (2001)
SeqA, the Escherichia coli origin sequestration protein, is also a specific transcription factor
Mol Microbiol, 40 (6), 1371-9
PubMed 11442835

Slominska M, Wegrzyn A, Konopa G, Skarstad K, Wegrzyn G (2001)
SeqA, the Escherichia coli origin sequestration protein, is also a specific transcription factor
Mol. Microbiol., 40 (6), 1371-1379

Zheng W, Li Z, Skarstad K, Crooke E (2001)
Mutations in DnaA protein suppress the growth arrest of acidic phospholipid-deficient Escherichia coli cells
EMBO J, 20 (5), 1164-72
PubMed 11230139

Publications 2000

Bernander R, Skarstad K (2000)
Mapping of a chromosome replication origin in an archaeon
Trends Microbiol, 8 (12), 535-7
PubMed 11115744

Boye E, Løbner-Olesen A, Skarstad K (2000)
Limiting DNA replication to once and only once
EMBO Rep, 1 (6), 479-83
PubMed 11263490

Skarstad K, Lueder G, Lurz R, Speck C, Messer W (2000)
The Escherichia coli SeqA protein binds specifically and co-operatively to two sites in hemimethylated and fully methylated oriC
Mol Microbiol, 36 (6), 1319-26
PubMed 10931282

Torheim NK, Boye E, Løbner-Olesen A, Stokke T, Skarstad K (2000)
The Escherichia coli SeqA protein destabilizes mutant DnaA204 protein
Mol Microbiol, 37 (3), 629-38
PubMed 10931356

Publications 1999

Torheim NK, Skarstad K (1999)
Escherichia coli SeqA protein affects DNA topology and inhibits open complex formation at oriC
EMBO J, 18 (17), 4882-8
PubMed 10469666

Publications 1998

Wold S, Boye E, Slater S, Kleckner N, Skarstad K (1998)
Effects of purified SeqA protein on oriC-dependent DNA replication in vitro
EMBO J, 17 (14), 4158-65
PubMed 9670030

Publications 1997

Stokke T, Smedshammer L, Jonassen TS, Blomhoff HK, Skarstad K, Steen HB (1997)
Uncoupling of the order of the S and M phases: effects of staurosporine on human cell cycle kinases
Cell Prolif, 30 (5), 197-218
PubMed 9420612

Publications 1996

Boye E, Stokke T, Kleckner N, Skarstad K (1996)
Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins
Proc Natl Acad Sci U S A, 93 (22), 12206-11
PubMed 8901558

Jair KW, Yu X, Skarstad K, Thöny B, Fujita N, Ishihama A, Wolf RE (1996)
Transcriptional activation of promoters of the superoxide and multiple antibiotic resistance regulons by Rob, a binding protein of the Escherichia coli origin of chromosomal replication
J Bacteriol, 178 (9), 2507-13
PubMed 8626315

Wold S, Crooke E, Skarstad K (1996)
The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro
Nucleic Acids Res, 24 (18), 3527-32
PubMed 8836178

Publications 1995

Skarstad K, Bernander R, Boye E (1995)
Analysis of DNA replication in vivo by flow cytometry
Methods Enzymol, 262, 604-13
PubMed 8594381

Skarstad K, Wold S (1995)
The speed of the Escherichia coli fork in vivo depends on the DnaB:DnaC ratio
Mol Microbiol, 17 (5), 825-31
PubMed 8596432

Slater S, Wold S, Lu M, Boye E, Skarstad K, Kleckner N (1995)
E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration
Cell, 82 (6), 927-36
PubMed 7553853

Publications 1994

Skarstad K, Boye E (1994)
The initiator protein DnaA: evolution, properties and function
Biochim Biophys Acta, 1217 (2), 111-30
PubMed 8110826

Steen HB, Jernaes MW, Skarstad K, Boye E (1994)
Staining and measurement of DNA in bacteria
Methods Cell Biol, 42 Pt B, 477-87
PubMed 7533253

Wold S, Skarstad K, Steen HB, Stokke T, Boye E (1994)
The initiation mass for DNA replication in Escherichia coli K-12 is dependent on growth rate
EMBO J, 13 (9), 2097-102
PubMed 8187762

Publications 1993

Skarstad K, Boye E (1993)
Degradation of individual chromosomes in recA mutants of Escherichia coli
J Bacteriol, 175 (17), 5505-9
PubMed 8366035

Skarstad K, Thöny B, Hwang DS, Kornberg A (1993)
A novel binding protein of the origin of the Escherichia coli chromosome
J Biol Chem, 268 (8), 5365-70
PubMed 8449900

Publications 1991

Crooke E, Hwang DS, Skarstad K, Thöny B, Kornberg A (1991)
E. coli minichromosome replication: regulation of initiation at oriC
Res Microbiol, 142 (2-3), 127-30
PubMed 1925009

Publications 1990

Skarstad K, Baker TA, Kornberg A (1990)
Strand separation required for initiation of replication at the chromosomal origin of E.coli is facilitated by a distant RNA--DNA hybrid
EMBO J, 9 (7), 2341-8
PubMed 1694129

Steen HB, Skarstad K, Boye E (1990)
DNA measurements of bacteria
Methods Cell Biol, 33, 519-26
PubMed 2084483

Publications 1989

Løbner-Olesen A, Skarstad K, Hansen FG, von Meyenburg K, Boye E (1989)
The DnaA protein determines the initiation mass of Escherichia coli K-12
Cell, 57 (5), 881-9
PubMed 2541928

Skarstad K, Løbner-Olesen A, Atlung T, von Meyenburg K, Boye E (1989)
Initiation of DNA replication in Escherichia coli after overproduction of the DnaA protein
Mol Gen Genet, 218 (1), 50-6
PubMed 2550764

Publications 1988

Boye E, Løbner-Olesen A, Skarstad K (1988)
Timing of chromosomal replication in Escherichia coli
Biochim Biophys Acta, 951 (2-3), 359-64
PubMed 2850013

Skarstad K, Boye E (1988)
Perturbed chromosomal replication in recA mutants of Escherichia coli
J Bacteriol, 170 (6), 2549-54
PubMed 3286612

Skarstad K, von Meyenburg K, Hansen FG, Boye E (1988)
Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles
J Bacteriol, 170 (2), 852-8
PubMed 2828328

Publications 1987

von Meyenburg K, Boye E, Skarstad K, Koppes L, Kogoma T (1987)
Mode of initiation of constitutive stable DNA replication in RNase H-defective mutants of Escherichia coli K-12
J Bacteriol, 169 (6), 2650-8
PubMed 3034862

Publications 1986

Skarstad K, Boye E, Steen HB (1986)
Timing of initiation of chromosome replication in individual Escherichia coli cells
EMBO J, 5 (7), 1711-7
PubMed 3527695

Steen HB, Skarstad K, Boye E (1986)
Flow cytometry of bacteria: cell cycle kinetics and effects of antibiotics
Ann N Y Acad Sci, 468, 329-38
PubMed 2942091

Publications 1985

Kogoma T, Skarstad K, Boye E, von Meyenburg K, Steen HB (1985)
RecA protein acts at the initiation of stable DNA replication in rnh mutants of Escherichia coli K-12
J Bacteriol, 163 (2), 439-44
PubMed 2991187

Skarstad K, Steen HB, Boye E (1985)
Escherichia coli DNA distributions measured by flow cytometry and compared with theoretical computer simulations
J Bacteriol, 163 (2), 661-8
PubMed 3894332

Publications 1984

Boye E, Köhnlein W, Skarstad K (1984)
Characterization of intracellular DNA strand breaks induced by neocarzinostatin in Escherichia coli cells
Nucleic Acids Res, 12 (21), 8281-91
PubMed 6239141

Publications 1983

Boye E, Steen HB, Skarstad K (1983)
Flow cytometry of bacteria: a promising tool in experimental and clinical microbiology
J Gen Microbiol, 129 (4), 973-80
PubMed 6350534

Skarstad K, Steen HB, Boye E (1983)
Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry
J Bacteriol, 154 (2), 656-62
PubMed 6341358

Publications 1982

Steen HB, Boye E, Skarstad K, Bloom B, Godal T, Mustafa S (1982)
Applications of flow cytometry on bacteria: cell cycle kinetics, drug effects, and quantitation of antibody binding
Cytometry, 2 (4), 249-57
PubMed 7035105

Publications 1981

Boye E, Alver S, Skarstad K (1981)
Deoxyribonucleic acid replication in permeable and fully viable Escherichia coli cells
J Bacteriol, 145 (3), 1413-6
PubMed 7009586