Norwegian Core Facility Available Services

LIST OF SERVICES

COSTS (VAT incl. for OUS users, VAT excl. for external users)

Use of culture facility

Fee-based by agreement, booking required
Use of virus lab Fee-based by agreement, booking required
Training in hiPSC cultivation 6000 NOK /person or 9000 NOK /two people, -  Reagents not included
Quarantine cell culturing prior to mycoplasma testing 100 NOK /day, booking required
Use of confocal microscope 100 NOK /hour, booking required
Human Feeders (irradiated) 20 NOK /35 mm dish
hESC / hiPSC cultivation Negotiable based on volume 

hiPSC generation (five clones depending on donor cell quality) from skin biopsy and blood sample

- banked fibroblast line from skin biopsy

- reprogrammed hiPSC line (Sendai virus)

- reprogrammed hiPSC line (ReproRNA-OKSGM)

- reprogrammed hiPSC line (Episomal)

 

 

1 000 NOK /line (2 cryotubes/biopsy)         

35 000 NOK /donor (4-5 iPSC lines/donor)        

35 000 NOK /donor (4-5 iPSC lines/donor)

35 000 NOK /donor (4-5 iPSC lines/donor)

Gene Editing (CRISPR)

- Knock Out editing

- Knock-in generation

- Point mutation (SNP) correction/insertion

- Reporter cell line generation

 

 

60 000 NOK /line     

70 000 NOK /line        

70 000 NOK /line                                                         

70-75 000 NOK /line (depending on the size of the reporter)                       

Five vials of two edited lines (0.5 mill. cells/vial) will be delivered

Media/reagents

- hESC medium

 

1000 NOK /500 mL

Mycoplasma detection in cell culture media 300 NOK /test
Cultivation of in-house /user provided hESC and hiPSCs Negotiable based on volume
Storage (liquid nitrogen) of hESC and hiPSCs 1000 NOK /year

Pluripotency testing of user-provided cells

- qPCR

- immunocytochemistry and confocal microscopy

 

400 NOK /plate (perishables fee-based by agreement)

1000 NOK /line

Differentiation via EB to three germ layers

2500 NOK /line

Genetic karyotyping of hES and hiPSCs

2500 NOK /line

Differentiation via hiPSCs to human neural progenitor cells (hNPCS)

3500 NOK/line

Flow cytometry

- Cell analysis

- DNA analysis

  

50 NOK / sample

100 NOK / sample 

Immunostaining of differentiated hESC and hiPSCs

Negotiable based on volume

Use of Variable Oxygen Cell Culture Unit (Biospherix)

- Gas supply (user dependent)

500 NOK / 50 L nitrogen gas
250 NOK / day
1000 NOK / week
3500 NOK / month