Norwegian Core Facility Available Services
| LIST OF SERVICES |
COSTS (VAT incl. for OUS users, VAT excl. for external users) |
|
Use of culture facility |
Fee-based by agreement, booking required |
| Use of virus lab | Fee-based by agreement, booking required |
| Training in hiPSC cultivation | 6000 NOK /person or 9000 NOK /two people, - Reagents not included |
| Quarantine cell culturing prior to mycoplasma testing | 100 NOK /day, booking required |
| Use of confocal microscope | 100 NOK /hour, booking required |
| Human Feeders (irradiated) | 20 NOK /35 mm dish |
| hESC / hiPSC cultivation | Negotiable based on volume |
|
hiPSC generation (five clones depending on donor cell quality) from skin biopsy and blood sample - banked fibroblast line from skin biopsy - reprogrammed hiPSC line (Sendai virus) - reprogrammed hiPSC line (ReproRNA-OKSGM) - reprogrammed hiPSC line (Episomal)
|
1 000 NOK /line (2 cryotubes/biopsy) 35 000 NOK /donor (4-5 iPSC lines/donor) 35 000 NOK /donor (4-5 iPSC lines/donor) 35 000 NOK /donor (4-5 iPSC lines/donor) |
|
Gene Editing (CRISPR) - Knock Out editing - Knock-in generation - Point mutation (SNP) correction/insertion - Reporter cell line generation
|
60 000 NOK /line 70 000 NOK /line 70 000 NOK /line 70-75 000 NOK /line (depending on the size of the reporter) Five vials of two edited lines (0.5 mill. cells/vial) will be delivered |
|
Media/reagents - hESC medium |
1000 NOK /500 mL |
| Mycoplasma detection in cell culture media | 300 NOK /test |
| Cultivation of in-house /user provided hESC and hiPSCs | Negotiable based on volume |
| Storage (liquid nitrogen) of hESC and hiPSCs | 1000 NOK /year |
|
Pluripotency testing of user-provided cells - qPCR - immunocytochemistry and confocal microscopy |
400 NOK /plate (perishables fee-based by agreement) 1000 NOK /line |
|
Differentiation via EB to three germ layers |
2500 NOK /line |
|
Genetic karyotyping of hES and hiPSCs |
2500 NOK /line |
|
Differentiation via hiPSCs to human neural progenitor cells (hNPCS) |
3500 NOK/line |
|
Flow cytometry - Cell analysis - DNA analysis |
50 NOK / sample 100 NOK / sample |
|
Immunostaining of differentiated hESC and hiPSCs |
Negotiable based on volume |
|
Use of Variable Oxygen Cell Culture Unit (Biospherix) - Gas supply (user dependent) |
500 NOK / 50 L nitrogen gas |