Protein array group

 
Group leader Fridtjof Lund-Johansen
Group leader Fridtjof Lund-Johansen

Aim: Develop array-based proteomics

The protein array laboratory is part of the KG Jebsen Center for Cancer Immunotherapy. Our aim is to develop next generation proteomics technology. Mass spectrometry (MS) has tremendous analytical power, but access is restricted by high cost and low throughput. We work extensively with MS and use the technology as reference for Microsphere Affinity Proteomics (MAP). MAP turns standard flow cytometers into high-speed proteomics instruments. MAP antibody arrays bring the power of proteomics into the hands of flow cytometry users.  During the development phase we are building alliances with the antibody industry with the aim of establishing the world’s largest collection of antibodies. MAP protein arrays with more than 10.000 full length human proteins was recently used to identify 50 new auto-antigens in a cohort of 300 patients with Primary Biliary Cirrhosis. Finally, arrays with peptide-MHC complexes open new possibilities for identification of effective T-cell antigens.

 
 

Projects

 
  • A bioinformatics platform for cellular proteomics
  • Measuring stability of MHC-peptide complexes
  • Array-based western blotting
  • Array-Large-scale analysis of protein complexes in subcellular compartments by antibody arrays and MS
  • Array-based-screening of auto-antibodies
 
 
 

Contact information:
Group leader Fridtjof Lund-Johansen, Department of Immunology, Oslo University Hospital, Rikshospitalet, Tel: +47 23073016, E-mail: fridtjof.lund-johansen@rr-research.no