Cecilie Jonsgar Sandberg

  • Post doc., PhD, MSc
  • +47 23071398
Identification of the gene expression profile in glioma stem cells

Gliomas are intrinsic brain tumors. They infiltrate the brain and usually recur after surgery. The most common type is astrocytoma grade IV or glioblastoma. This is a notoriously malignant tumor with median survival of about 12 months. We and others have identified a cell in these tumors that behave similar to a stem cells. We suspect that the tumor recurs and kills the patient due to that such tumor stem cells (TSCs) are left in the brain when the patient is operated.

Identification of unique properties in the TSCs may lead to therapy for the patients. I am especially concerned with signalling pathways controlling the proliferation and differentiation of these cells.

I grow TSCs isolated from low grade astrocyttomas and glioblastomas in vitro as spheres in serum-free media with TGFâ and FGF2. The cells are differentiated by withdrawal of mitogens and adding serum to the culture media. RNA is isolated from tissue, spheres and differentiated cells. The RNA is reverse transcribed to cDNA and further used for gene expression analysis.

The results from these expression studies will be used to identify and understand the most important signalling mechanisms involved in TSC proliferation, invasion etc.
 

Publications 2023

Brynjulvsen M, Solli E, Walewska M, Zucknick M, Djirackor L, Langmoen IA, Mughal AA, Skaga E, Vik-Mo EO, Sandberg CJ (2023)
Functional and Molecular Heterogeneity in Glioma Stem Cells Derived from Multiregional Sampling
Cancers (Basel), 15 (24)
DOI 10.3390/cancers15245826, PubMed 38136371

Publications 2022

Skaga E, Kulesskiy E, Potdar S, Panagopoulos I, Micci F, Langmoen IA, Sandberg CJ, Vik-Mo EO (2022)
Functional temozolomide sensitivity testing of patient-specific glioblastoma stem cell cultures is predictive of clinical outcome
Transl Oncol, 26, 101535
DOI 10.1016/j.tranon.2022.101535, PubMed 36115076

Publications 2021

Djirackor L, Halldorsson S, Niehusmann P, Leske H, Capper D, Kuschel LP, Pahnke J, Due-Tønnessen BJ, Langmoen IA, Sandberg CJ, Euskirchen P, Vik-Mo EO (2021)
Intraoperative DNA methylation classification of brain tumors impacts neurosurgical strategy
Neurooncol Adv, 3 (1), vdab149
DOI 10.1093/noajnl/vdab149, PubMed 34729487

Publications 2020

Kierulf-Vieira KS, Sandberg CJ, Waaler J, Lund K, Skaga E, Saberniak BM, Panagopoulos I, Brandal P, Krauss S, Langmoen IA, Vik-Mo EO (2020)
A Small-Molecule Tankyrase Inhibitor Reduces Glioma Stem Cell Proliferation and Sphere Formation
Cancers (Basel), 12 (6)
DOI 10.3390/cancers12061630, PubMed 32575464

Publications 2019

Hvinden IC, Berg HE, Sachse D, Skaga E, Skottvoll FS, Lundanes E, Sandberg CJ, Vik-Mo EO, Rise F, Wilson SR (2019)
Nuclear Magnetic Resonance Spectroscopy to Identify Metabolite Biomarkers of Nonresponsiveness to Targeted Therapy in Glioblastoma Tumor Stem Cells
J Proteome Res, 18 (5), 2012-2020
DOI 10.1021/acs.jproteome.8b00801, PubMed 30964684

Skaga E, Kulesskiy E, Brynjulvsen M, Sandberg CJ, Potdar S, Langmoen IA, Laakso A, Gaál-Paavola E, Perola M, Wennerberg K, Vik-Mo EO (2019)
Feasibility study of using high-throughput drug sensitivity testing to target recurrent glioblastoma stem cells for individualized treatment
Clin Transl Med, 8 (1), 33
DOI 10.1186/s40169-019-0253-6, PubMed 31889236

Skaga E, Kulesskiy E, Fayzullin A, Sandberg CJ, Potdar S, Kyttälä A, Langmoen IA, Laakso A, Gaál-Paavola E, Perola M, Wennerberg K, Vik-Mo EO (2019)
Intertumoral heterogeneity in patient-specific drug sensitivities in treatment-naïve glioblastoma
BMC Cancer, 19 (1), 628
DOI 10.1186/s12885-019-5861-4, PubMed 31238897

Skaga E, Skaga IØ, Grieg Z, Sandberg CJ, Langmoen IA, Vik-Mo EO (2019)
The efficacy of a coordinated pharmacological blockade in glioblastoma stem cells with nine repurposed drugs using the CUSP9 strategy
J Cancer Res Clin Oncol, 145 (6), 1495-1507
DOI 10.1007/s00432-019-02920-4, PubMed 31028540

Publications 2018

Fayzullin A, Sandberg CJ, Spreadbury M, Saberniak BM, Grieg Z, Skaga E, Langmoen IA, Vik-Mo EO (2018)
Phenotypic and Expressional Heterogeneity in the Invasive Glioma Cells
Transl Oncol, 12 (1), 122-133
DOI 10.1016/j.tranon.2018.09.014, PubMed 30292065

Skottvoll FS, Berg HE, Bjørseth K, Lund K, Roos N, Bekhradnia S, Thiede B, Sandberg C, Vik-Mo EO, Roberg-Larsen H, Nyström B, Lundanes E, Wilson SR (2018)
Ultracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations
Future Sci OA, 5 (1), FSO359
DOI 10.4155/fsoa-2018-0088, PubMed 30652024

Publications 2015

Joel M, Mughal AA, Grieg Z, Murrell W, Palmero S, Mikkelsen B, Fjerdingstad HB, Sandberg CJ, Behnan J, Glover JC, Langmoen IA, Stangeland B (2015)
Targeting PBK/TOPK decreases growth and survival of glioma initiating cells in vitro and attenuates tumor growth in vivo
Mol Cancer, 14, 121
DOI 10.1186/s12943-015-0398-x, PubMed 26081429

Kierulf-Vieira KS, Sandberg CJ, Grieg Z, Günther CC, Langmoen IA, Vik-Mo EO (2015)
Wnt inhibition is dysregulated in gliomas and its re-establishment inhibits proliferation and tumor sphere formation
Exp Cell Res, 340 (1), 53-61
DOI 10.1016/j.yexcr.2015.12.010, PubMed 26712519

Stangeland B, Mughal AA, Grieg Z, Sandberg CJ, Joel M, Nygård S, Meling T, Murrell W, Vik Mo EO, Langmoen IA (2015)
Combined expressional analysis, bioinformatics and targeted proteomics identify new potential therapeutic targets in glioblastoma stem cells
Oncotarget, 6 (28), 26192-215
DOI 10.18632/oncotarget.4613, PubMed 26295306

Publications 2014

Sandberg CJ, Vik-Mo EO, Behnan J, Helseth E, Langmoen IA (2014)
Transcriptional profiling of adult neural stem-like cells from the human brain
PLoS One, 9 (12), e114739
DOI 10.1371/journal.pone.0114739, PubMed 25514637

Publications 2013

Joel M, Sandberg CJ, Boulland JL, Vik-Mo EO, Langmoen IA, Glover JC (2013)
Inhibition of tumor formation and redirected differentiation of glioblastoma cells in a xenotypic embryonic environment
Dev Dyn, 242 (9), 1078-93
DOI 10.1002/dvdy.24001, PubMed 23780720

Murrell W, Palmero E, Bianco J, Stangeland B, Joel M, Paulson L, Thiede B, Grieg Z, Ramsnes I, Skjellegrind HK, Nygård S, Brandal P, Sandberg C, Vik-Mo E, Palmero S, Langmoen IA (2013)
Expansion of multipotent stem cells from the adult human brain
PLoS One, 8 (8), e71334
DOI 10.1371/journal.pone.0071334, PubMed 23967194

Sandberg CJ, Altschuler G, Jeong J, Strømme KK, Stangeland B, Murrell W, Grasmo-Wendler UH, Myklebost O, Helseth E, Vik-Mo EO, Hide W, Langmoen IA (2013)
Comparison of glioma stem cells to neural stem cells from the adult human brain identifies dysregulated Wnt- signaling and a fingerprint associated with clinical outcome
Exp Cell Res, 319 (14), 2230-43
DOI 10.1016/j.yexcr.2013.06.004, PubMed 23791939

Vik-Mo EO, Nyakas M, Mikkelsen BV, Moe MC, Due-Tønnesen P, Suso EM, Sæbøe-Larssen S, Sandberg C, Brinchmann JE, Helseth E, Rasmussen AM, Lote K, Aamdal S, Gaudernack G, Kvalheim G, Langmoen IA (2013)
Therapeutic vaccination against autologous cancer stem cells with mRNA-transfected dendritic cells in patients with glioblastoma
Cancer Immunol Immunother, 62 (9), 1499-509
DOI 10.1007/s00262-013-1453-3, PubMed 23817721

Publications 2012

Fossdal G, Vik-Mo EO, Sandberg C, Varghese M, Kaarbø M, Telmo E, Langmoen IA, Murrell W (2012)
Aqp 9 and brain tumour stem cells
ScientificWorldJournal, 2012, 915176
DOI 10.1100/2012/915176, PubMed 22262958

Publications 2011

Vik-Mo EO, Sandberg C, Joel M, Stangeland B, Watanabe Y, Mackay-Sim A, Moe MC, Murrell W, Langmoen IA (2011)
A comparative study of the structural organization of spheres derived from the adult human subventricular zone and glioblastoma biopsies
Exp Cell Res, 317 (7), 1049-59
DOI 10.1016/j.yexcr.2010.12.022, PubMed 21199649

Publications 2010

Vik-Mo EO, Sandberg C, Olstorn H, Varghese M, Brandal P, Ramm-Pettersen J, Murrell W, Langmoen IA (2010)
Brain tumor stem cells maintain overall phenotype and tumorigenicity after in vitro culturing in serum-free conditions
Neuro Oncol, 12 (12), 1220-30
DOI 10.1093/neuonc/noq102, PubMed 20843775

Publications 2008

Moe MC, Kolberg RS, Sandberg C, Vik-Mo E, Olstorn H, Varghese M, Langmoen IA, Nicolaissen B (2008)
A comparison of epithelial and neural properties in progenitor cells derived from the adult human ciliary body and brain
Exp Eye Res, 88 (1), 30-8
DOI 10.1016/j.exer.2008.09.020, PubMed 18955049

Varghese M, Olstorn H, Sandberg C, Vik-Mo EO, Noordhuis P, Nistér M, Berg-Johnsen J, Moe MC, Langmoen IA (2008)
A comparison between stem cells from the adult human brain and from brain tumors
Neurosurgery, 63 (6), 1022-33; discussion 1033-4
DOI 10.1227/01.NEU.0000335792.85142.B0, PubMed 19057315

Publications 2006

Tollefsen KE, Bratsberg E, Bøyum O, Finne EF, Gregersen IK, Hegseth M, Sandberg C, Hylland K (2006)
Use of fish in vitro hepatocyte assays to detect multi-endpoint toxicity in Slovenian river sediments
Mar Environ Res, 62 Suppl, S356-9
DOI 10.1016/j.marenvres.2006.04.025, PubMed 16697455

Tollefsen KE, Finne EF, Romstad R, Sandberg C (2006)
Effluents from oil production activities contain chemicals that interfere with normal function of intra- and extra-cellular estrogen binding proteins
Mar Environ Res, 62 Suppl, S191-4
DOI 10.1016/j.marenvres.2006.04.006, PubMed 16697461

Publications 2004

Øie E, Vinge LE, Andersen GØ, Yndestad A, Krobert KA, Sandberg C, Ahmed MS, Haug T, Levy FO, Skomedal T, Attramadal H (2004)
RAMP2 and RAMP3 mRNA levels are increased in failing rat cardiomyocytes and associated with increased responsiveness to adrenomedullin
J Mol Cell Cardiol, 38 (1), 145-51
DOI 10.1016/j.yjmcc.2004.10.009, PubMed 15623431

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