Anette Weyergang
Position: Senior scientist, Ph.D.
Phone: +47 22 78 14 81
Email:

Address

Department of Radiation Biology
Institute for Cancer Research
Norwegian Radium Hospital
Oslo University Hospital
Montebello
0310 Oslo, Norway


CURRICULUM VITAE

Dr philos Anette Weyergang

Degree-Granting Education

2009 University of Oslo, Norway, Dr. philos./ PhD, Pharmacy
PhD project: Photochemical internalization of epidermal growth factor receptor targeted drugs
Project place: Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital

2003 University of Oslo, Norway, Cand. pharm./M. Pharm

Postdoc training

2009- Research fellow at Department of Radiation Biology, Institute for Cancer Research
Norwegian Radium Hospital, Oslo, Norway (Grants from The Norwegian Cancer
Society)

2009: Sister Insstitution associate post doctor researcher at department of Experimental Therapeutics, MD Anderson Cancer Center, Houston, Texas in Dr Rosenblums research group.

RESEARCH INTERESTS

My fields of interest are pharmacy, drug delivery, experimental cancer therapy and photomedicine. I am involved as a postdoctoral research fellow in the group of Photochemical Internalization (PCI). PCI is a novel method for drug delivery to cells.

Methods used: tissue culture, photodynamic therapy, photochemical internalization, toxicity assays (MTT, relative protein synthesis measurements, clonal cell survival, Coulter Counter measurments), siRNA transfection, cell free reticulocytt lysate system for ribosome inhibiting activity of toxins, fluorescence microscopy, PCR, SDS-PAGE, Western blotting, production (Fermentation), isolation and purification (Immobilized Metal Affinity Chromatography (IMAC) and Ion Exchange Chromatography with SP Sepharose) of recombinant proteins, LAL test for determination of endotoxins, measurement of cellular ROS generation, ELASA, LAL for determination of endotoxins. In vivo work with BALB C and BALB C nu/nu mice documenting PCI of targeted toxins.

SCIENTIFIC PRODUCTION

-13 original publications (research manuscripts) in per reviewed international journals
-7 Review articles in per reviewed international journals
-3 book chapters
-2 thesis (one doctoral- and one master-thesis)
-1 patent

REVIEWER IN:

Journal of controlled release
Cell Biochemistry & Function
International Journal of Cancer
Photochemical & Photobiological Sciences

TEACHING

Local contribution:
-Min Supervisor for 2 master student in our lab (Norwegian Radium Hospital) 2011-2014
-Co-supervision of 3 master students in our lab (Norwegian Radium Hospital) 2007-20011
-Currently main supervisor for three PhD students in our lab
-Teaching of students from Ullern upper secondary school in photobiology and photomedicine as a part of Oslo Cancer Cluster 2010-2012
-Course in photomedicine for bachelor-students in biophysics from Norwegian University of Science and Technology (NTNU) 2011

Regional, National or International Contributions
-Invited oral presentations (2) at School of Pharmacy, University of Oslo 2009
-Invited oral presentation (1) at the 9th International Symposium on Photodynamic Therapy and Photodiagnosis in Clinical Practice, Bressanone, Italy 2012
-Invited oral presentation at the 15th Congress of the European Society for Photobiology, Liege, Belgium, 2013

EXTERNAL FOUND RAISING
2008 Grant for a 3 years post doc position from the Norwegian Cancer Society (my former position)
2011 Grant for a 3 years PhD scholarship from the Norwegian Cancer Society (provided one of my PhD students Bente Bull-Hansen
2011 Grant for a 3 years PhD scholarship from South-Eastern Norway Regional Health Authority (provided to one of my PhD students Maria EB Berstad)
2012 Grant for a 4th year in a post doc position from the Norwegian Cancer Society

AWARDS

2007 Award for the best oral presentation in the Minisymposia on Research and development at Days of Pharmacy days 2007
2009 Travel Award from The Norwegian Cancer Society for a 3 months scientific stay at MD Anderson Cancer Center, Houston, Texas

MEMBERSHIP IN SOCIETIES

• Norwegian Society for Biochemistry
• Norwegian Society for Photobiology and Photomedicine
• European Society for Photobiology
• Norwegian Society for Pharmacy
• American Association for Cancer Research

REFERENCES

Dr.philos. Kristian Berg,
Department of Radiation Biology
Institute for Cancer Research - Norwegian Radium Hospital
Rikshospitalet University Hospital
Montebello
N-0310 Oslo
Norway
Phone: (+47) 22 78 14 79
Fax: (+47) 22 78 14 95
E-mail: kristian.berg@rr-research.no

Michael G. Rosenblum, Ph.D.
Professor of Medicine
Head, Immunopharmacology and Targeted Therapy Laboratory
Director of Research Development
Department of Experimental Therapeutics
MD Anderson Cancer Center
Houston, Texas
713-792-3554(O)
713-794-4261(FAX)
713-404-3362(PAGE)
E-mail: mrosenbl@manderson.org

 

 

Author network for Anette Weyergang by COREMINE medical


Publications 2017

Norum OJ, Fremstedal ASV, Weyergang A, Golab J, Berg K (2017)
Photochemical delivery of bleomycin induces T-cell activation of importance for curative effect and systemic anti-tumor immunity
J Control Release (in press)
PubMed 29042319

Olsen CE, Weyergang A, Edwards VT, Berg K, Brech A, Weisheit S, Høgset A, Selbo PK (2017)
Development of resistance to photodynamic therapy (PDT) in human breast cancer cells is photosensitizer-dependent: Possible mechanisms and approaches for overcoming PDT-resistance
Biochem Pharmacol, 144, 63-77
PubMed 28784290

Publications 2015

Berstad MB, Cheung LH, Berg K, Peng Q, Fremstedal AS, Patzke S, Rosenblum MG, Weyergang A (2015)
Design of an EGFR-targeting toxin for photochemical delivery: in vitro and in vivo selectivity and efficacy
Oncogene, 34 (44), 5582-92
PubMed 25684137

Bull-Hansen B, Berstad MB, Berg K, Cao Y, Skarpen E, Fremstedal AS, Rosenblum MG, Peng Q, Weyergang A (2015)
Photochemical activation of MH3-B1/rGel: a HER2-targeted treatment approach for ovarian cancer
Oncotarget, 6 (14), 12436-51
PubMed 26002552

Selbo PK, Bostad M, Olsen CE, Edwards VT, Høgset A, Weyergang A, Berg K (2015)
Photochemical internalisation, a minimally invasive strategy for light-controlled endosomal escape of cancer stem cell-targeting therapeutics
Photochem Photobiol Sci, 14 (8), 1433-50
PubMed 25805311

Weyergang A, Berstad ME, Bull-Hansen B, Olsen CE, Selbo PK, Berg K (2015)
Photochemical activation of drugs for the treatment of therapy-resistant cancers
Photochem Photobiol Sci, 14 (8), 1465-75
PubMed 25849953

Publications 2014

Bostad M, Kausberg M, Weyergang A, Olsen CE, Berg K, Høgset A, Selbo PK (2014)
Light-triggered, efficient cytosolic release of IM7-saporin targeting the putative cancer stem cell marker CD44 by photochemical internalization
Mol Pharm, 11 (8), 2764-76
PubMed 24960585

Bull-Hansen B, Cao Y, Berg K, Skarpen E, Rosenblum MG, Weyergang A (2014)
Photochemical activation of the recombinant HER2-targeted fusion toxin MH3-B1/rGel; Impact of HER2 expression on treatment outcome
J Control Release, 182, 58-66
PubMed 24637464

Weyergang A, Cheung LH, Rosenblum MG, Mohamedali KA, Peng Q, Waltenberger J, Berg K (2014)
Photochemical internalization augments tumor vascular cytotoxicity and specificity of VEGF(121)/rGel fusion toxin
J Control Release, 180, 1-9
PubMed 24531010

Publications 2013

Olsen CE, Berg K, Selbo PK, Weyergang A (2013)
Circumvention of resistance to photodynamic therapy in doxorubicin-resistant sarcoma by photochemical internalization of gelonin
Free Radic Biol Med, 65, 1300-9
PubMed 24076428

Vikdal M, Weyergang A, Selbo PK, Berg K (2013)
Vascular endothelial cells as targets for photochemical internalization (PCI)
Photochem Photobiol, 89 (5), 1185-92
PubMed 23819771

Weyergang A, Selbo PK, Berg K (2013)
Sustained ERK [corrected] inhibition by EGFR targeting therapies is a predictive factor for synergistic cytotoxicity with PDT as neoadjuvant therapy
Biochim Biophys Acta, 1830 (3), 2659-70
PubMed 23671927

Weyergang A, Selbo PK, Berg K (2013)
Sustained ERK inhibition by EGFR targeting therapies is a predictive factor for synergistic cytotoxicity with PDT as neoadjuvant therapy (vol 1830, pg 2659, 2013)
Biochim. Biophys. Acta-Gen. Subj., 1830 (10), 4417

Publications 2012

Berstad MB, Weyergang A, Berg K (2012)
Photochemical internalization (PCI) of HER2-targeted toxins: synergy is dependent on the treatment sequence
Biochim Biophys Acta, 1820 (12), 1849-58
PubMed 22981913

Selbo PK, Weyergang A, Eng MS, Bostad M, Mælandsmo GM, Høgset A, Berg K (2012)
Strongly amphiphilic photosensitizers are not substrates of the cancer stem cell marker ABCG2 and provides specific and efficient light-triggered drug delivery of an EGFR-targeted cytotoxic drug
J Control Release, 159 (2), 197-203
PubMed 22349185

Publications 2011

Weyergang A, Selbo PK, Berstad ME, Bostad M, Berg K (2011)
Photochemical internalization of tumor-targeted protein toxins
Lasers Surg Med, 43 (7), 721-33
PubMed 22057500

Publications 2010

Berg K, Berstad M, Prasmickaite L, Weyergang A, Selbo PK, Hedfors I, Høgset A (2010)
Photochemical internalization: a new tool for gene and oligonucleotide delivery
Top Curr Chem, 296, 251-81
PubMed 21504105

Berg K, Weyergang A, Prasmickaite L, Bonsted A, Høgset A, Strand MT, Wagner E, Selbo PK (2010)
Photochemical internalization (PCI): a technology for drug delivery
Methods Mol Biol, 635, 133-45
PubMed 20552345

Selbo PK, Weyergang A, Høgset A, Norum OJ, Berstad MB, Vikdal M, Berg K (2010)
Photochemical internalization provides time- and space-controlled endolysosomal escape of therapeutic molecules
J Control Release, 148 (1), 2-12
PubMed 20600406

Publications 2009

Norum OJ, Selbo PK, Weyergang A, Giercksky KE, Berg K (2009)
Photochemical internalization (PCI) in cancer therapy: from bench towards bedside medicine
J Photochem Photobiol B, 96 (2), 83-92
PubMed 19467605

Weyergang A, Berg K, Kaalhus O, Peng Q, Selbo PK (2009)
Photodynamic therapy targets the mTOR signaling network in vitro and in vivo
Mol Pharm, 6 (1), 255-64
PubMed 19125612

Publications 2008

Weyergang A, Kaalhus O, Berg K (2008)
Photodynamic therapy with an endocytically located photosensitizer cause a rapid activation of the mitogen-activated protein kinases extracellular signal-regulated kinase, p38, and c-Jun NH2 terminal kinase with opposing effects on cell survival
Mol Cancer Ther, 7 (6), 1740-50
PubMed 18566245

Weyergang A, Kaalhus O, Berg K (2008)
Photodynamic targeting of EGFR does not predict the treatment outcome in combination with the EGFR tyrosine kinase inhibitor Tyrphostin AG1478
Photochem Photobiol Sci, 7 (9), 1032-40
PubMed 18754049

Publications 2007

Berg K, Folini M, Prasmickaite L, Selbo PK, Bonsted A, Engesaeter BØ, Zaffaroni N, Weyergang A, Dietze A, Maelandsmo GM, Wagner E, Norum OJ, Høgset A (2007)
Photochemical internalization: a new tool for drug delivery
Curr Pharm Biotechnol, 8 (6), 362-72
PubMed 18289045

Weyergang A, Selbo PK, Berg K (2007)
Y1068 phosphorylation is the most sensitive target of disulfonated tetraphenylporphyrin-based photodynamic therapy on epidermal growth factor receptor
Biochem Pharmacol, 74 (2), 226-35
PubMed 17531956

Yip WL, Weyergang A, Berg K, Tønnesen HH, Selbo PK (2007)
Targeted delivery and enhanced cytotoxicity of cetuximab-saporin by photochemical internalization in EGFR-positive cancer cells
Mol Pharm, 4 (2), 241-51
PubMed 17263556

Publications 2006

Berg K, Høgset A, Prasmickaite L, Weyergang A, Bonsted A, Dietze A, Lou PJ, Bown S, Norum OJ, Møllergard HM, Selbo PK (2006)
Photochemical internalization (PCI): A novel technology for activation of endocytosed therapeutic agents.
Med Laser Appl, 21, 239-251
PublikaID 73

Dietze A, Selbo PK, Prasmickaite L, Weyergang A, Bonsted A, Engesaeter B, Hogset A, Berg K (2006)
Photochemical internalization (PCI): a new modality for light activation of endocytosed therapeuticals
J Environ Pathol Toxicol Oncol, 25 (1-2), 521-36
PubMed 16566739

Selbo PK, Weyergang A, Bonsted A, Bown SG, Berg K (2006)
Photochemical internalization of therapeutic macromolecular agents: a novel strategy to kill multidrug-resistant cancer cells
J Pharmacol Exp Ther, 319 (2), 604-12
PubMed 16902053

Weyergang A, Selbo PK, Berg K (2006)
Photochemically stimulated drug delivery increases the cytotoxicity and specificity of EGF-saporin
J Control Release, 111 (1-2), 165-73
PubMed 16466823

Publications 2005

Berg K, Selbo PK, Weyergang A, Dietze A, Prasmickaite L, Bonsted A, Engesaeter BØ, Angell-Petersen E, Warloe T, Frandsen N, Høgset A (2005)
Porphyrin-related photosensitizers for cancer imaging and therapeutic applications
J Microsc, 218 (Pt 2), 133-47
PubMed 15857375