Pål Kristian Selbo
Position: Project leader, Senior scientist, PhD
Phone: +47 22 78 14 69
Email:

Fax: +47 22 78 14 95
Room: K03 071

Curriculum vitae

Address

Department of Radiation Biology
Institute for Cancer Research
Norwegian Radium Hospital
Oslo University Hospital
Montebello
0310 Oslo, Norway

Degree-Granting Education

2001 University of Oslo, Norway, Dr. philos./ PhD, Biochemistry

1994 University of Trondheim, Norway, Cand. scient./MS, Cell Biology

1993 University of Trondheim, Norway, BS, Biology

PhD project: Photochemical internalisation: a novel drug delivery system.
Project place: Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital

Postdoc training

2001-2002:  
Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway (Grants from The Norwegian Radium Hospital Research Foundation)
2002-2003:Research Fellow at Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, USA (Grants from NIH/NCI)
2004-2007:Research Fellow at Department of Radiation Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway (Grants from The Norwegian Cancer Society)


Honors & Awards

2013 Claude Rimington Commemorative Prize

Societies

Norwegian Biochemical Society, Norwegian Society for Photobiology and Photomedicine, European Society for Photobiology, American Society for Photobiology

Administration and Boards

1999-2002:Member of the board of the Norwegian Society for Photobiology and Photomedicine
2002-2003:Member of the teaching committee at the Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, USA
2006-2010: 
Secretary of the Norwegian Society for Photobiology and Photomedicine
2008-2009Co-chairman for the Institute seminars at the Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital
2007-present:  
Project leader. Affiliated to the Cancer Stem Cell Innovation Center (SFI) at Oslo University Hospital.
2010- presentPresident of the Norwegian Society for Photobiology and Photomedicine

Reviewer for following journals

Antibodies; Biochemical Pharmacology; BMC Biotechnology; British Journal of Cancer; Cancer Immunology and Immunotherapy; Cancer Letters; Cancer Research; Drug Design, Development and Therapy; Expert Opinion on Therapeutic Patents; Histochemistry and Cell Biology; Journal of Controlled Release; Molecular Cancer Therapeutics, Molecular Pharmaceutics; PLoS ONE, Photochemical & Photobiological Sciences; Toxins.

Publications, patents and research interests

50 publications, including 44 peer reviewed papers (se under) and 3 filed patents.

Research interests

Photomedicine, Photochemical internalization, Photodynamic therapy, Controlled drug delivery and Experimental cancer therapy

Description of primary research

PCI is a minimal invasive, highly specific and efficient technology for light-enhanced cytosolic release of membrane-impermeable molecular therapeutics entrapped in endocytic vesicles. Briefly, the drug or gene of interest colocalizes with a photosensitizer in endocytic vesicles. Light-activation of the photosensitizer results in reactive oxygen species (ROS)-mediated damage of the membranes of these vesicles with the subsequent release of the drug or gene to cytosol. PCI has been demonstrated to be a feasible drug delivery technology in >80 different cancer cell lines and in >10 different animal models. PCI has been shown to be a highly efficient technology for induced endolysosomal escape for several cancer targeted therapeutics and to increase the efficacy of some conventional anti-cancer agents.
Review: Selbo PK et al., J Control Release, 2010

Present projects:
I) PCI as a novel approach to selectively target end eradicate cancer stem cells
II) Boosting the efficacy of therapeutic anticancer vaccines by PCI

 

Author network for Pål Kristian Selbo by COREMINE medical


Publications 2017

Olsen CE, Weyergang A, Edwards VT, Berg K, Brech A, Weisheit S, Høgset A, Selbo PK (2017)
Development of resistance to photodynamic therapy (PDT) in human breast cancer cells is photosensitizer-dependent: Possible mechanisms and approaches for overcoming PDT-resistance
Biochem Pharmacol, 144, 63-77
PubMed 28784290

Publications 2015

Bostad M, Olsen CE, Peng Q, Berg K, Høgset A, Selbo PK (2015)
Light-controlled endosomal escape of the novel CD133-targeting immunotoxin AC133-saporin by photochemical internalization - A minimally invasive cancer stem cell-targeting strategy
J Control Release, 206, 37-48
PubMed 25758331

Selbo PK, Bostad M, Olsen CE, Edwards VT, Høgset A, Weyergang A, Berg K (2015)
Photochemical internalisation, a minimally invasive strategy for light-controlled endosomal escape of cancer stem cell-targeting therapeutics
Photochem Photobiol Sci, 14 (8), 1433-50
PubMed 25805311

Weyergang A, Berstad ME, Bull-Hansen B, Olsen CE, Selbo PK, Berg K (2015)
Photochemical activation of drugs for the treatment of therapy-resistant cancers
Photochem Photobiol Sci, 14 (8), 1465-75
PubMed 25849953

Publications 2014

Bostad M, Kausberg M, Weyergang A, Olsen CE, Berg K, Høgset A, Selbo PK (2014)
Light-triggered, efficient cytosolic release of IM7-saporin targeting the putative cancer stem cell marker CD44 by photochemical internalization
Mol Pharm, 11 (8), 2764-76
PubMed 24960585

Håkerud M, Selbo PK, Waeckerle-Men Y, Contassot E, Dziunycz P, Kündig TM, Høgset A, Johansen P (2014)
Photosensitisation facilitates cross-priming of adjuvant-free protein vaccines and stimulation of tumour-suppressing CD8 T cells
J Control Release, 198, 10-7
PubMed 25482339

Lund K, Bostad M, Skarpen E, Braunagel M, Kiprijanov S, Krauss S, Duncan A, Høgset A, Selbo PK (2014)
The novel EpCAM-targeting monoclonal antibody 3-17I linked to saporin is highly cytotoxic after photochemical internalization in breast, pancreas and colon cancer cell lines
MAbs, 6 (4), 1038-50
PubMed 24525727

Repetto-Llamazares AH, Larsen RH, Giusti AM, Riccardi E, Bruland ØS, Selbo PK, Dahle J (2014)
177Lu-DOTA-HH1, a novel anti-CD37 radio-immunoconjugate: a study of toxicity in nude mice
PLoS One, 9 (7), e103070
PubMed 25068508

Publications 2013

Bostad M, Berg K, Høgset A, Skarpen E, Stenmark H, Selbo PK (2013)
Photochemical internalization (PCI) of immunotoxins targeting CD133 is specific and highly potent at femtomolar levels in cells with cancer stem cell properties
J Control Release, 168 (3), 317-26
PubMed 23567040

Håkerud M, Waeckerle-Men Y, Selbo PK, Kündig TM, Høgset A, Johansen P (2013)
Intradermal photosensitisation facilitates stimulation of MHC class-I restricted CD8 T-cell responses of co-administered antigen
J Control Release, 174, 143-50
PubMed 24280261

Olsen CE, Berg K, Selbo PK, Weyergang A (2013)
Circumvention of resistance to photodynamic therapy in doxorubicin-resistant sarcoma by photochemical internalization of gelonin
Free Radic Biol Med, 65, 1300-9
PubMed 24076428

Stratford EW, Bostad M, Castro R, Skarpen E, Berg K, Høgset A, Myklebost O, Selbo PK (2013)
Photochemical internalization of CD133-targeting immunotoxins efficiently depletes sarcoma cells with stem-like properties and reduces tumorigenicity
Biochim Biophys Acta, 1830 (8), 4235-43
PubMed 23643966

Vikdal M, Weyergang A, Selbo PK, Berg K (2013)
Vascular endothelial cells as targets for photochemical internalization (PCI)
Photochem Photobiol, 89 (5), 1185-92
PubMed 23819771

Weyergang A, Selbo PK, Berg K (2013)
Sustained ERK [corrected] inhibition by EGFR targeting therapies is a predictive factor for synergistic cytotoxicity with PDT as neoadjuvant therapy
Biochim Biophys Acta, 1830 (3), 2659-70
PubMed 23671927

Weyergang A, Selbo PK, Berg K (2013)
Sustained ERK inhibition by EGFR targeting therapies is a predictive factor for synergistic cytotoxicity with PDT as neoadjuvant therapy (vol 1830, pg 2659, 2013)
Biochim. Biophys. Acta-Gen. Subj., 1830 (10), 4417

Publications 2012

Abu-Yousif AO, Moor AC, Zheng X, Savellano MD, Yu W, Selbo PK, Hasan T (2012)
Epidermal growth factor receptor-targeted photosensitizer selectively inhibits EGFR signaling and induces targeted phototoxicity in ovarian cancer cells
Cancer Lett, 321 (2), 120-7
PubMed 22266098

Selbo PK, Weyergang A, Eng MS, Bostad M, Mælandsmo GM, Høgset A, Berg K (2012)
Strongly amphiphilic photosensitizers are not substrates of the cancer stem cell marker ABCG2 and provides specific and efficient light-triggered drug delivery of an EGFR-targeted cytotoxic drug
J Control Release, 159 (2), 197-203
PubMed 22349185

Publications 2011

Berg K, Nordstrand S, Selbo PK, Tran DT, Angell-Petersen E, Høgset A (2011)
Disulfonated tetraphenyl chlorin (TPCS2a), a novel photosensitizer developed for clinical utilization of photochemical internalization
Photochem Photobiol Sci, 10 (10), 1637-51
PubMed 21773635

Weyergang A, Selbo PK, Berstad ME, Bostad M, Berg K (2011)
Photochemical internalization of tumor-targeted protein toxins
Lasers Surg Med, 43 (7), 721-33
PubMed 22057500

Publications 2010

Berg K, Berstad M, Prasmickaite L, Weyergang A, Selbo PK, Hedfors I, Høgset A (2010)
Photochemical internalization: a new tool for gene and oligonucleotide delivery
Top Curr Chem, 296, 251-81
PubMed 21504105

Berg K, Weyergang A, Prasmickaite L, Bonsted A, Høgset A, Strand MT, Wagner E, Selbo PK (2010)
Photochemical internalization (PCI): a technology for drug delivery
Methods Mol Biol, 635, 133-45
PubMed 20552345

Johansen P, Berg K, Selbo PK, Hofbauer GF (2010)
[Photochemical internalisation (PCI): a further development of photodynamic therapy for the treatment of skin cancer]
Praxis (Bern 1994), 99 (23), 1423-8
PubMed 21082595

Selbo PK, Weyergang A, Høgset A, Norum OJ, Berstad MB, Vikdal M, Berg K (2010)
Photochemical internalization provides time- and space-controlled endolysosomal escape of therapeutic molecules
J Control Release, 148 (1), 2-12
PubMed 20600406

Publications 2009

Norum OJ, Selbo PK, Weyergang A, Giercksky KE, Berg K (2009)
Photochemical internalization (PCI) in cancer therapy: from bench towards bedside medicine
J Photochem Photobiol B, 96 (2), 83-92
PubMed 19467605

Selbo PK, Rosenblum MG, Cheung LH, Zhang W, Berg K (2009)
Multi-modality therapeutics with potent anti-tumor effects: photochemical internalization enhances delivery of the fusion toxin scFvMEL/rGel
PLoS One, 4 (8), e6691
PubMed 19690617

Weyergang A, Berg K, Kaalhus O, Peng Q, Selbo PK (2009)
Photodynamic therapy targets the mTOR signaling network in vitro and in vivo
Mol Pharm, 6 (1), 255-64
PubMed 19125612

Woodhams J, Lou PJ, Selbo PK, Mosse A, Oukrif D, MacRobert A, Novelli M, Peng Q, Berg K, Bown SG (2009)
Intracellular re-localisation by photochemical internalisation enhances the cytotoxic effect of gelonin--quantitative studies in normal rat liver
J Control Release, 142 (3), 347-53
PubMed 19932724

Publications 2007

Berg K, Folini M, Prasmickaite L, Selbo PK, Bonsted A, Engesaeter BØ, Zaffaroni N, Weyergang A, Dietze A, Maelandsmo GM, Wagner E, Norum OJ, Høgset A (2007)
Photochemical internalization: a new tool for drug delivery
Curr Pharm Biotechnol, 8 (6), 362-72
PubMed 18289045

Melhus KB, Larsen RH, Stokke T, Kaalhus O, Selbo PK, Dahle J (2007)
Evaluation of the binding of radiolabeled rituximab to CD20-positive lymphoma cells: an in vitro feasibility study concerning low-dose-rate radioimmunotherapy with the alpha-emitter 227Th
Cancer Biother Radiopharm, 22 (4), 469-79
PubMed 17803441

Weyergang A, Selbo PK, Berg K (2007)
Y1068 phosphorylation is the most sensitive target of disulfonated tetraphenylporphyrin-based photodynamic therapy on epidermal growth factor receptor
Biochem Pharmacol, 74 (2), 226-35
PubMed 17531956

Yip WL, Weyergang A, Berg K, Tønnesen HH, Selbo PK (2007)
Targeted delivery and enhanced cytotoxicity of cetuximab-saporin by photochemical internalization in EGFR-positive cancer cells
Mol Pharm, 4 (2), 241-51
PubMed 17263556

Publications 2006

Berg K, Høgset A, Prasmickaite L, Weyergang A, Bonsted A, Dietze A, Lou PJ, Bown S, Norum OJ, Møllergard HM, Selbo PK (2006)
Photochemical internalization (PCI): A novel technology for activation of endocytosed therapeutic agents.
Med Laser Appl, 21, 239-251
PublikaID 73

Dietze A, Selbo PK, Prasmickaite L, Weyergang A, Bonsted A, Engesaeter B, Hogset A, Berg K (2006)
Photochemical internalization (PCI): a new modality for light activation of endocytosed therapeuticals
J Environ Pathol Toxicol Oncol, 25 (1-2), 521-36
PubMed 16566739

Selbo PK, Weyergang A, Bonsted A, Bown SG, Berg K (2006)
Photochemical internalization of therapeutic macromolecular agents: a novel strategy to kill multidrug-resistant cancer cells
J Pharmacol Exp Ther, 319 (2), 604-12
PubMed 16902053

Solban N, Selbo PK, Sinha AK, Chang SK, Hasan T (2006)
Mechanistic investigation and implications of photodynamic therapy induction of vascular endothelial growth factor in prostate cancer
Cancer Res, 66 (11), 5633-40
PubMed 16740700

Weyergang A, Selbo PK, Berg K (2006)
Photochemically stimulated drug delivery increases the cytotoxicity and specificity of EGF-saporin
J Control Release, 111 (1-2), 165-73
PubMed 16466823

Publications 2005

Berg K, Selbo PK, Weyergang A, Dietze A, Prasmickaite L, Bonsted A, Engesaeter BØ, Angell-Petersen E, Warloe T, Frandsen N, Høgset A (2005)
Porphyrin-related photosensitizers for cancer imaging and therapeutic applications
J Microsc, 218 (Pt 2), 133-47
PubMed 15857375

Dietze A, Peng Q, Selbo PK, Kaalhus O, Müller C, Bown S, Berg K (2005)
Enhanced photodynamic destruction of a transplantable fibrosarcoma using photochemical internalisation of gelonin
Br J Cancer, 92 (11), 2004-9
PubMed 15886704

Publications 2004

Berg K, Selbo PK, Prasmickaite L, Høgset A (2004)
Photochemical drug and gene delivery
Curr Opin Mol Ther, 6 (3), 279-87
PubMed 15264430

Høgset A, Prasmickaite L, Selbo PK, Hellum M, Engesaeter BØ, Bonsted A, Berg K (2004)
Photochemical internalisation in drug and gene delivery
Adv Drug Deliv Rev, 56 (1), 95-115
PubMed 14706447

Publications 2003

Berg K, Prasmickaite L, Selbo PK, Hellum M, Bonsted A, Høgset A (2003)
Photochemical internalization (PCI)--a novel technology for release of macromolecules from endocytic vesicles
Oftalmologia, 56 (1), 67-71
PubMed 12886687

Høgset A, Prasmickaite L, Engesaeter BO, Hellum M, Selbo PK, Olsen VM, Maelandsmo GM, Berg K (2003)
Light directed gene transfer by photochemical internalisation
Curr Gene Ther, 3 (2), 89-112
PubMed 12653404

Publications 2002

Prasmickaite L, Høgset A, Selbo PK, Engesaeter BØ, Hellum M, Berg K (2002)
Photochemical disruption of endocytic vesicles before delivery of drugs: a new strategy for cancer therapy
Br J Cancer, 86 (4), 652-7
PubMed 11870551

Selbo PK, Høgset A, Prasmickaite L, Berg K (2002)
Photochemical internalisation: a novel drug delivery system
Tumour Biol, 23 (2), 103-12
PubMed 12065848

Publications 2001

Selbo PK, Kaalhus O, Sivam G, Berg K (2001)
5-Aminolevulinic acid-based photochemical internalization of the immunotoxin MOC31-gelonin generates synergistic cytotoxic effects in vitro
Photochem Photobiol, 74 (2), 303-10
PubMed 11547569

Selbo PK, Sivam G, Fodstad O, Sandvig K, Berg K (2001)
In vivo documentation of photochemical internalization, a novel approach to site specific cancer therapy
Int J Cancer, 92 (5), 761-6
PubMed 11340584

Publications 2000

Selbo PK, Sandvig K, Kirveliene V, Berg K (2000)
Release of gelonin from endosomes and lysosomes to cytosol by photochemical internalization
Biochim Biophys Acta, 1475 (3), 307-13
PubMed 10913830

Selbo PK, Sivam G, Fodstad O, Sandvig K, Berg K (2000)
Photochemical internalisation increases the cytotoxic effect of the immunotoxin MOC31-gelonin
Int J Cancer, 87 (6), 853-9
PubMed 10956397

Publications 1999

Berg K, Selbo PK, Prasmickaite L, Tjelle TE, Sandvig K, Moan J, Gaudernack G, Fodstad O, Kjølsrud S, Anholt H, Rodal GH, Rodal SK, Høgset A (1999)
Photochemical internalization: a novel technology for delivery of macromolecules into cytosol
Cancer Res, 59 (6), 1180-3
PubMed 10096543

Publications 1997

Gaullier JM, Berg K, Peng Q, Anholt H, Selbo PK, Ma LW, Moan J (1997)
Use of 5-aminolevulinic acid esters to improve photodynamic therapy on cells in culture
Cancer Res, 57 (8), 1481-6
PubMed 9108449

Publications 1996

Andersen S, Bjermer L, Selbo PK, Dalaker M, Johansen B (1996)
Extracellular phospholipase A2 expression in sarcoidosis
Sarcoidosis Vasc Diffuse Lung Dis, 13 (1), 70-6
PubMed 8865415