The core facility for Advanced Electron Microscopy - Montebello
The core facility for Advanced Electron Microscopy at OUH is a state-of–the-art equipped laboratory that offers high-end imaging technology for researchers. The facility contains two nodes which, are localized at the Pathology Dept., Rikshospitalet and the Institute for Cancer Research, Department of Molecular Cell Biology, Radium Hospital. The node at Rikshospitalet serves researchers at Rikshospitalet, Ullevål Hospital and the Gaustad University campus, and shares equipment with the diagnostic unit. The node at the Radium Hospital mainly serves researchers at the Institute for Cancer Research and the Radium Hospital.
The core facility offers specialized equipment and preparation methods for advanced electron microscopy of cells and tissues. Both labs offer preparation methods for pure morphological studies and for immunocytochemical localization of antigens on ultrathin sections. Samples can be further quantitatively evaluated using various forms of morphometric analysis. For more information please see link (methods). More advanced techniques such as high-pressure freezing followed by freeze substitution, correlative light electron microscopy (CLEM) and electron tomography are also available. We offer training for users in both specimen preparation and microscopy.
Our own research:
The study of cellular mechanism such as intracellular traffic, growth factor receptor sorting and signaling demands a thorough understanding of the subcellular localization of the involved proteins. It is our principal goal to implement electron microscopical techniques in the ongoing research within the Department of Molecular Cell Biology to facilitate this task.
- Sorting of proteins within endosomes
- Autophagy in the fruitfly drosophila melanogaster
- The role of autophagy in the removal of protein aggregates
- The role of phosphoinositides in receptor sorting and multivesicular body formation
We use EM to study cellular morphology and protein localization. To do so we use several techniques, ranging from conventional plastic embedding to immunocytochemistry on cryosections. The latter technique allows us to visualize antigen localization on sections with the help of antibodies and protein A-gold particles.
Andreas Brech, Department of Molecular Cell Biology, Institute for Cancer Research
The Norwegian Radium Hospital, Montebello, 0379 Oslo, Norway
Phone +47 22 93 42 83 Fax: +47 22 50 86 92, Switchboard: +47 22 93 40 00