Tumor hypoxia is the primary cause of radiation resistance in several histological types of cancer. The main aim of the project is to develop a non-invasive method for detection of hypoxic regions within tumors. Our hypothesis is that dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) may be a useful method.
Amelanotic human melanomas xenografted into BALB/c-nu/nu mice are used as preclinical models of human cancer. Clinical studies are performed in canine tumors and human cervix carcinomas. DCE-MRI is performed at 1.5 T by using a clinical MR-tomograph. Gd-DTPA is used as contrast agent. Oxygen tension is measured by using oxygen electrodes and hypoxia markers (pimonidazole).