Window Chamber Tumors

Image of a window chamber preparation recorded after a bolus of fluorescence-labeled dextran had been administered i.v. in the host mouse. Vessels filled with fluorescence-labeled dextran have high intensity. The tumor microvascular network is clearly visible, and can be subjected to detailed analysis.
Image of a window chamber preparation recorded after a bolus of fluorescence-labeled dextran had been administered i.v. in the host mouse. Vessels filled with fluorescence-labeled dextran have high intensity. The tumor microvascular network is clearly visible, and can be subjected to detailed analysis.

Our group has developed a transparent window chamber technique for studying tumor growth, angiogenesis, cell migration, and cell intravasation. The transparent window chamber is inserted surgically into a skin fold on the dorsum of athymic mice. Multicellular spheroids or single cell suspensions are inoculated into intradermal tissue within the chamber. A light microscope and high-resolution CCD-cameras are used for observation. Single tumor cells and the movement of erythrocytes within tumor capillaries can easily be observed. Used in combination with fluorescence techniques, the window chamber system is also well suited for studies of the uptake of macromolecular therapeutic agents in tumor tissue.
 
Jan 2, 2004 Page visits: 5112