Our group has developed a transparent window chamber technique for studying tumor growth, angiogenesis, cell migration, and cell intravasation. The transparent window chamber is inserted surgically into a skin fold on the dorsum of athymic mice. Multicellular spheroids or single cell suspensions are inoculated into intradermal tissue within the chamber. A light microscope and high-resolution CCD-cameras are used for observation. Single tumor cells and the movement of erythrocytes within tumor capillaries can easily be observed. Used in combination with fluorescence techniques, the window chamber system is also well suited for studies of the uptake of macromolecular therapeutic agents in tumor tissue.
Intravital microscopy of tumor vessel morphology and function using a standard fluorescence microscope
Eur J Nucl Med Mol Imaging
DCE-MRI of Tumor Hypoxia and Hypoxia-Associated Aggressiveness
Cancers (Basel), 12 (7)
Antifibrotic therapy to normalize the tumor microenvironment
J Transl Med, 18 (1), 207