SNPs with proven functional effect and clinical relevance for the response to the following anticancer drugs: 5-Fluorouracyl, Doxorubicin, Metothrexate, Etoposide, 6-Mercaptopurine, Azothiopurin, Thioguanine, Irinotekan are studied in this project. The high precision genotyping of these SNPs is performed on the Nanogen platform. Genotyping on the Nanogen platform involves attachment of a biotinylated PCR product to a chip coated with hydrogel enriched with streptavidin, which is addressed by a stabilizer and a fluorescent labelled probe reporting for each of the variant alleles.
Figure A. Principle of SNP detection with the Nanogen system. Genotyping involves attaching a biotinylated PCR product to a streptavidin coated chip. A fluorescent probe that reports the respective allele is addressed to the amplicon. Alternatively, the probe can be attached to the solid surface and addressed by the PCR product (click to enlarge).
Key members: Silje Nordgard og Grethe I. Grenaker Alnæs
Tahiri A, Tekpli X, Satheesh SV, DeWijn R, Lüders T, Bukholm IR, Hurtado A, Geisler J, Kristensen VN(2020) Loss of progesterone receptor is associated with distinct tyrosine kinase profiles in breast cancer Breast Cancer Res Treat DOI 10.1007/s10549-020-05763-7, PubMed 32710281
Bergholtz H, Kumar S, Wärnberg F, Lüders T, Kristensen V, Sørlie T(2020) Comparable cancer-relevant mutation profiles in synchronous ductal carcinoma in situ and invasive breast cancer Cancer Rep (Hoboken), 3(3), e1248 DOI 10.1002/cnr2.1248, PubMed 32671987
Liu J, Prager-van der Smissen WJC, Collée JM, Bolla MK, Wang Q, Michailidou K, Dennis J, Ahearn TU, Aittomäki K, Ambrosone CB, Andrulis IL, Anton-Culver H, Antonenkova NN, Arndt V, Arnold N, Aronson KJ, Augustinsson A, Auvinen P, Becher H, Beckmann MW, Behrens S, Bermisheva M, Bernstein L, Bogdanova NV, Bogdanova-Markov Net al.(2020) Germline HOXB13 mutations p.G84E and p.R217C do not confer an increased breast cancer risk Sci Rep, 10(1), 9688 DOI 10.1038/s41598-020-65665-y, PubMed 32546843