Our research activities are divided into three subprojects and include studies of effects of ionizing radiation on malignant cells and tissues, as well as studies of physiological and biological processes in tumors of relevance for radiation therapy of cancer. The aims of the three subprojects are:
I. To establish diagnostic methods for detecting radiation resistant tumors due to hypoxic regions or high interstitial fluid pressure.
II. To use intravital microscopy for studying tumor angiogenesis and physiology, and to develop therapeutic strategies for overcoming hypoxia-induced radiation resistance.
III. To discover mechanisms of microenvironment- and/or radiation therapy-induced metastasis.
The experiments are being performed with human melanomas and cervix carcinomas (cell lines, multicellular spheroids, xenografts, tumor biopsies and patients with cervix carcinomas). Important methods include intravital microscopy, pO2 histography (Eppendorf and OxyLite electrodes), HbO2 microspectrophotometry, wick-in-needle and micropipette measurements of interstitial fluid pressure, MR imaging and spectroscopy, and several techniques for studying the molecular biology of tumors).
Intratumor Heterogeneity in Interstitial Fluid Pressure in Cervical and Pancreatic Carcinoma Xenografts
Transl Oncol, 12 (8), 1079-1085 (in press)
DCE-MRI and Quantitative Histology Reveal Enhanced Vessel Maturation but Impaired Perfusion and Increased Hypoxia in Bevacizumab-Treated Cervical Carcinoma
Int J Radiat Oncol Biol Phys, 104 (3), 666-676
Pharmacokinetic analysis of DCE-MRI data of locally advanced cervical carcinoma with the Brix model
Acta Oncol, 58 (6), 828-837