Ingvild Sørum Leikfoss

  • Researcher; PhD, MSc
  • +47 230 79021

Address: Oslo University Hospital, Research Unit for Neuroscience, Domus Medica II, 2nd floor, L-260, Gaustadalleen 34, 0372 Oslo

Background:
Ingvild Sørum Leikfoss has a PhD degree in Molecular Immunology from the University of Oslo from 2015, with the following title “Characterization of multiple sclerosis susceptibility genes”. She has a Master of Science degree in Molecular Biology and Immunology from the Department of Molecular Biosciences. The focus of her master thesis was in vitro characterization of the binding between Immunoglobulin G and different Fc receptors from both humans and mice.

Project/research interests
The main focus of her project is to analyze a biomarker for neurodegeneration in MS patients. She is also involved in several projects within the group and have a broad experience using different immunological and molecular laboratory techniques such as cell culturing and activation (primary human cells and cell lines), ELISA, Flow cytometry, Image stream (Image based flow cytometry), biomarker analysis, Western blotting, Chromatin Immunoprecipitation (ChIP), qPCR and cloning. Leikfoss is also a co-worker in the EU-projects MultipleMS, where she is responsible for handling and processing biological material collected from Norwegian MS patients included in the prospective part of the study, and she is a member of the projects Innovation Management Team. In addition, Leikfoss is responsible for coordinating the local MS research biobank.

Methodology:
• Cultivation and transfection of cell lines (Jurkat T cells, Raji B cells)
• Isolation and cultivation of primary, human T - and B- cells
• Western blotting
• Flow cytometry
• Image stream
• RNA and DNA isolation
• Quantitative real-time PCR
• Sub-cloning
• ELISA

 

Publications 2019

Brorson IS, Eriksson A, Leikfoss IS, Celius EG, Berg-Hansen P, Barcellos LF, Berge T, Harbo HF, Bos SD (2019)
No differential gene expression for CD4+ T cells of MS patients and healthy controls
Mult Scler J Exp Transl Clin, 5 (2), 2055217319856903
DOI 10.1177/2055217319856903, PubMed 31223483

Publications 2016

Berge T, Leikfoss IS, Brorson IS, Bos SD, Page CM, Gustavsen MW, Bjølgerud A, Holmøy T, Celius EG, Damoiseaux J, Smolders J, Harbo HF, Spurkland A (2016)
The multiple sclerosis susceptibility genes TAGAP and IL2RA are regulated by vitamin D in CD4+ T cells
Genes Immun, 17 (2), 118-27
DOI 10.1038/gene.2015.61, PubMed 26765264

Publications 2015

Bos SD, Page CM, Andreassen BK, Elboudwarej E, Gustavsen MW, Briggs F, Quach H, Leikfoss IS, Bjølgerud A, Berge T, Harbo HF, Barcellos LF (2015)
Genome-wide DNA methylation profiles indicate CD8+ T cell hypermethylation in multiple sclerosis
PLoS One, 10 (3), e0117403
DOI 10.1371/journal.pone.0117403, PubMed 25734800

Leikfoss IS, Keshari PK, Gustavsen MW, Bjølgerud A, Brorson IS, Celius EG, Spurkland A, Bos SD, Harbo HF, Berge T (2015)
Multiple Sclerosis Risk Allele in CLEC16A Acts as an Expression Quantitative Trait Locus for CLEC16A and SOCS1 in CD4+ T Cells
PLoS One, 10 (7), e0132957
DOI 10.1371/journal.pone.0132957, PubMed 26203907

Publications 2014

Eriksen AB, Berge T, Gustavsen MW, Leikfoss IS, Bos SD, Spurkland A, Harbo HF, Blomhoff HK (2014)
Retinoic acid enhances the levels of IL-10 in TLR-stimulated B cells from patients with relapsing-remitting multiple sclerosis
J Neuroimmunol, 278, 11-8
DOI 10.1016/j.jneuroim.2014.11.019, PubMed 25595247

Publications 2013

Berge T, Leikfoss IS, Harbo HF (2013)
From Identification to Characterization of the Multiple Sclerosis Susceptibility Gene CLEC16A
Int J Mol Sci, 14 (3), 4476-97
DOI 10.3390/ijms14034476, PubMed 23439554

Publications 2012

Andersen JT, Foss S, Kenanova VE, Olafsen T, Leikfoss IS, Roopenian DC, Wu AM, Sandlie I (2012)
Anti-carcinoembryonic antigen single-chain variable fragment antibody variants bind mouse and human neonatal Fc receptor with different affinities that reveal distinct cross-species differences in serum half-life
J Biol Chem, 287 (27), 22927-37
DOI 10.1074/jbc.M112.355131, PubMed 22570488

Leikfoss IS, Mero IL, Dahle MK, Lie BA, Harbo HF, Spurkland A, Berge T (2012)
Multiple sclerosis-associated single-nucleotide polymorphisms in CLEC16A correlate with reduced SOCS1 and DEXI expression in the thymus
Genes Immun, 14 (1), 62-6
DOI 10.1038/gene.2012.52, PubMed 23151489

Page visits: 4712