Sony SH800 Cell Sorter (Montebello)

A truly easy to use cell sorter, with automatic allignment and sort parameter calibration. It is equipped with a 488nm and a 638nm laser, both have a regulated output of 15 mW. The Sony SH800 has a co-linear laser set-up with FSC, backscatter (≈SSC) and 6 fluorescence detectors. This user operated cell sorter is based on disposable sorting chips (70, 100 or 130 μm) as a combined flow cell and nozzle.

FCCF users can be trained to operate it 24/7. Contact flowcytometri@rr-research.no to become a user. Booking for trained users at icr-booking.no. 

Sample tubes: microtubes (0.5mL or 1.5mL),12 x 75-mm, and 15-mL tubes.
Temperature Control: cooling or warming for collection tubes and sort sample.
Collection devices:
- Two-way sorting into 12 x 75 mm, and 15 mL tubes.
- One-way sorting (including single cell) onto slides and into multiwell plates or PCR-plates.

Room: K03.080

Fee DIY sorting (1 hour minimum booking for each user):

• Academic user within Helse Sør-Øst (HSØ) region: 350 kr/h (ex. mva, non-HSØ account requires mva)
• Academic user outside HSØ: 525 kr/h (ex.mva)
• Commercial user: 1400 kr/h (ex.mva)

Fee when sorted by employees in the core facility: 

• Academic user within Helse Sør-Øst (HSØ) region: 700 kr/h (ex. mva, non-HSØ account requires mva)
• Academic user outside HSØ: 1050 kr/h (ex.mva)
• Commercial user: 2800 kr/h (ex.mva)

Examples of fluorochromes that can be used:

*FL 4, FL 5 and FL 6 detectors is common for blue and red laser excitation! Do not choose a staining setup that combines fluorochromes with the same emission spectra due to the co-linear laser system on this instrument.

E.g. PE-Cy7 and APC-Cy7 in the same sample. The Sony SH800 cannot separate the signal from these two!

Ask the personnel at the FCCF for help with the setup.