Example of multiparameter immunofluorescence

Human bone marrow stained with IgM FITC, CD34 PE, CD19 PE-Cy5, CD10 PE-Cy7 and CD20 APC.

Viable, single cells positive for CD10 and CD19 were gated out, and of these cells we were interested in the CD34 negative cells. Based on the IgM and CD20 content of these cells we could separate immature, small and large pre B-cells .

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