Current targets of Cdk1 in budding yeast

 

Over 70 Cdk1 targets have been identified in S. cerevisiae, with functions in diverse processes involved in cell cycle progression.

 

Name ORF Function  Cdk1 target reference
Regulation of Cdk1 activity  
Cln1 YMR199W  G1 cyclin, promotes the G1-S phase transition  
Cln2 YPL256C G1 cyclin, promotes the G1-S phase transition   
Cln3 YAL040C  G1 cyclin, promotes the G1-S phase transition  MCB 13(6):3266-71
Clb6 YGR109C  B-type cyclin involved in DNA replication  
Far1 YJL157C  Cyclin-dependent kinase inhibitor  
Sic1 YLR079W  Cyclin-dependent kinase inhibitor  
Swe1 YJL187C  Protein kinase that inhibits G2/M transition by phosphorylating Cdk1  
Mih1 YMR036C  Phosphatase that promotes G2/M transition by dephosphorylating Cdk1  
       
DNA replication  
Sld2 YKL108W Protein required for DNA replication, forms complex with Dpb11  
Sld3 YGL113W Assembly of replication proteins at the replication origins; binds Cdc45  
Pol12 YBL035C B-subunit of DNA polymerase alpha-primase complex  
Cdc6 YJL194W Required for association of the Mcm2-7 complex to DNA; DNA replication  
Orc2 YBR060C Subunit of the origin recognition complex; initiation of DNA replication  
Orc6 YHR118C Subunit of the origin recognition complex; initiation of DNA replication  
Mcm3 YEL032W  Component of the Mcm2-7 complex;  initiation of DNA replication   
Dpb2 YPR175W Subunit of DNA polymerase II (DNA polymerase epsilon); DNA replication  
       
Anaphase-promoting complex  
Cdh1  YGL003C Cell-cycle regulated activator of the anaphase-promoting complex  
Acm1  YPL267W Pseudosubstrate inhibitor of the anaphase-promoting complex  
Cdc16  YKL022C Subunit of the anaphase-promoting complex  
Cdc23  YHR166C Subunit of the anaphase-promoting complex  
Cdc27  YBL084C Subunit of the anaphase-promoting complex  
       
Pheromone signaling   
Ste20  YHL007C PAK-like kinase involved in pheromone response and polarized cell growth  
Ste5  YDR103W Pheromone-response scaffold protein; binds Ste11, Ste7 and Fus3 kinases  
       
Transcription   
Ace2  YLR131C Daughter cell specific transcription factor of early G1-specific genes  
Whi5  YOR083W pRb-like repressor of G1 transcription  
Swi6  YLR182W Transcription factor that regulates transcription at the G1/S transition  
Swi5  YDR146C Transcription factor of genes expressed at the M/G1 phase boundary  
Fkh2  YNL068C Forkhead transcription factor controlling the expression of G2/M phase genes  
Ndd1  YOR372C Transcriptional activator that functions in combination with Fkh2  
Stb1  YNL309W Protein with a role in regulation of transcription at G1-S phase boundary  
Pho2  YDL106C Homeobox transcription factor; controls genes involved in phosphate metabolism  
Sum1 YDR310C Transcriptional repressor required for mitotic repression of middle sporulation-specific genes Shin et al, 2010
       
Initiation of anaphase, FEAR pathway, Mitotic exit network
Pds1  YDR113C Securin, inhibits anaphase by binding and inhibiting Esp1 (separin)   
Cdc5  YMR001C Polo-like kinase; has multiple functions in mitosis and cytokinesis  
Net1  YJL076W Inhibits mitotic exit by sequestering the phosphatase Cdc14 in the nucleolus  
Spo12 YHR152W  Stimulates mitotic exit by inducing release of Cdc14 from the nucleolus in early anaphase  
Lte1  YAL024C Guanine nucleotide exchange factor (GEF) for Tem1, which is a key regulator of mitotic exit  
Cdc15  YAR019C  Kinase that promotes mitotic exit by directly switching on the kinase activity of Dbf2  
       
DNA repair, maintenance of telomeres  
Srs2  YJL092W DNA helicase involved in DNA repair  
Sae2  YGL175C Endonuclease involved in resection of DNA double strand breaks, which is the first step in homologous recombination  
Cdc13  YDL220C Telomere capping protein, involved in telomere elongation   
Mer2  YJR021C Coordinates the initiation of recombination and the first division of meiosis; part of a complex (Mer2-Mei4-Rec114)  
       
Regulation of SPBs, kinetochores, microtubules, mitotic spindle assembly  
Ase1  YOR058C Microtubule-associated protein (MAP); required for spindle elongation and stabilization  
Ask1  YKL052C Subunit of the DASH complex, couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation  
Bir1  YJR089W Subunit of chromosomal passenger complex which regulates chromosome segregation  
Cin8 YEL061C Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Chee and Haase, 2010
Cnm67  YNL225C Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration  
Fin1  YDR130C Intermediate filament protein, forms cell cycle-specific filaments between spindle pole bodies  
Kar9  YPL269W Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules  
Kip1 YBL063W Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning Chee and Haase, 2010
Mps1  YDL028K Kinase required for spindle pole body duplication and spindle checkpoint function  
Nud1  YOR373W Component of the spindle pole body outer plaque, required for exit from mitosis  
Sli15  YBR156C Subunit of the chromosomal passenger complex which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly  
Slk19  YOR195W Kinetochore-associated protein required for chromsome segregation, component of FEAR network which induces mitotic exit  
Spc110  YDR356W Inner plaque spindle pole body component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB  
Spc42  YKL042W Central plaque component of spindle pole body; involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane  
Stu2  YLR045C Microtubule-associated protein (MAP); regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment  
       

Bud site selection, cell polarity, secretion, bud growth, organelle inheritance 
Bem1  YBR200W Involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24, Ste5, Ste20, and Bud1  
Bem2  YER155C Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis  
Bem3  YPL115C Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42, which controls establishment and maintenance of cell polarity, including bud-site assembly  
Boi1  YBL085W Protein implicated in polar growth, interacts with Bem1, contains an SH3 domain and a PH domain  
Boi2  YER114C Involved in polar growth, interacts with Bem1, contains SH3 and PH domains  
Rga2  YDR379W GTPase-activating protein for the polarity-establishment protein Cdc42  
Tgl4 YKR089C Triacylglycerol lipase involved in triacylglycerol mobilization and degradation  
Tus1 YLR425W Rho1 Guanine nucleotide exchange factor (GEF)  
Vac17 YCL063W Vacuole inheritance  
Smp2 YMR165C Phosphatase; dephosphorylates phosphatidate to yield diacylglycerol and Pi  
       
Septins      
Cdc3 YLR314C Component of the septin ring of the mother-bud neck that is required for cytokinesis  
Shs1 YDL225W Component of the septin ring of the mother-bud neck that is required for cytokinesis  
       
Other  
Nup53 YMR153W Subunit of the nuclear pore complex, interacts with the karyopherin Kap121 and with Nup170  

 









 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

 
 
 

 
 
 
 
 
 
 
 
 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


 
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