Fridtjof Lund-Johansen
Position: Group leader, MD PhD
Phone: +47 23073016

MD PhD, Leading a group working on array-based protein analysis. Our goal is to make proteomics simple and accessible. In Microsphere Affinity Proteomics (MAP) biotinylated sample proteins are captured onto bead-based antibody arrays and detected by flow cytometry. MAP has a multiplexing capacity similar to that of mass spectrometry but the throughput is at least two orders of magnitude higher. We are currently developing a multiplexed version of the western blot and complete set of MAP assays to analyze proteins and protein complexes in subcellular compartments. We are also developing a MAP array with thousands of recombinant human proteins. The MAP protein array will be used to test the specificity of antibodies to human proteins and to screen for autoantibodies in serum from patients with auto-immune disease and cancer. To validate MAP, we make extensive use of mass spectrometry. We generate reference data sets that are as lookup tables to identify antibody targets that correspond to specific binding or cross-reactivity, respectively. MAP technology is a game changer in antibody validation and protein detection. Lund-Johansen and co-workers have published work on MAP technology published in Molecular&Cellular Proteomics (2009, 2016), Proteomics (2011), New Biotechnology (2012)and Cytometry (2012). More recently, the group has published new bioinformatics for subcellular proteomics (Nature Methods in press)

Publika - Scientific publications from Oslo University Hospital
Publika - Scientific publications from Oslo University Hospital

Publika - scientific publications from Oslo University Hospital

Publika is a database of scientific publications by authors affiliated with Oslo University Hospital.

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Our 5 most recent articles

Magne J, Bucciarelli-Ducci C, Dahl JS, Gimelli A, Haugaa KH, Miller O, Muraru D, Donal E, Edvardsen T, Popescu BA (2018)
EuroEcho-imaging 2017: highlights
Eur Heart J Cardiovasc Imaging (in press)
PubMed 29548013

Kim SJ, Huzarski T, Gronwald J, Singer CF, Møller P, Lynch HT, Armel S, Karlan BY, Foulkes WD, Neuhausen SL, Senter L, Eisen A, Eng C, Panchal S, Pal T, Olopade O, Zakalik D, Lubinski J, Narod SA, Kotsopoulos J, Hereditary Breast Cancer Clinical Study Group (2018)
Prospective evaluation of body size and breast cancer risk among BRCA1 and BRCA2 mutation carriers
Int J Epidemiol (in press)
PubMed 29547931

Bjørnland K, Hinna M, Aksnes G, Stensrud KJ, Ertresvåg K, Bjørnstad-Østensen A, Sanengen T, Line PD, Aagenæs I, Aabakken L, Emblem R, Almaas R (2018)
Outcome for biliary atresia patients treated at a low-volume centre
Scand J Gastroenterol, 1-4 (in press)
PubMed 29546790

Farnes I, Johnson E, Johannessen HO (2017)
Management of gastric conduit retention following hybrid and minimally invasive esophagectomy for esophageal cancer: Two retrospective case series
Int J Surg Case Rep, 41, 505-510
PubMed 29546028

Henriksen HB, Carlsen MH, Paur I, Berntsen S, Bøhn SK, Skjetne AJ, Kværner AS, Henriksen C, Andersen LF, Smeland S, Blomhoff R (2018)
Relative validity of a short food frequency questionnaire assessing adherence to the Norwegian dietary guidelines among colorectal cancer patients
Food Nutr Res, 62
PubMed 29545734