Antoni Wiedlocha's group - Protein internalisation and signaling
Nuclear translocation and intracrine signaling of FGFs
Altered cell signaling in growth regulation is a prerequisite for cancer development. Our group studies fibroblast growth factor (FGF) signaling mechanisms and the translocation of FGF1 and FGF2 into cells. We found that, in addition to the well known mechanism of signaling through the tyrosine kinase receptor, FGF1 similarly to FGF2 is able to translocate into cells to fulfill the signal. We are going to understand in details mechanisms of FGF action including (i) activation of signaling network, internalization and down-regulation of activated FGF receptors. (ii) FGF1 and FGF2 translocation into the cytosol and nucleus, (iii) identification of new intracellular FGF1 and FGF2 binding partners. New findings in this field would be beneficial for finding new molecular targets for anti-cancer therapy.
Antoni Wiedlocha's Group click to enlarge image
Department of Biochemistry, Institute for Cancer Research
The Norwegian Radium Hospital, Montebello, 0379 Oslo, Norway
Phone +47 22 78 19 30 (Wiedlocha), Switchboard: +47 22 93 40 00
Zakrzewska M, Haugsten EM, Nadratowska-Wesolowska B, Oppelt A, Hausott B, Jin Y, Otlewski J, Wesche J, Wiedlocha A (2013)
ERK-Mediated Phosphorylation of Fibroblast Growth Factor Receptor 1 on Ser777 Inhibits Signaling.
Sci Signal. 6(262)
23405013 Free pdf
Zhen Y, Sørensen V, Skjerpen CS, Haugsten EM, Jin Y, Wälchli S, Olsnes S, Wiedlocha A (2012)
Nuclear import of exogenous FGF1 requires the ER-protein LRRC59 and the importins Kpnα1 and Kpnβ1.
Traffic. 2012 Feb 9. [Epub ahead of print]
Jin Y, Zhen Y, Haugsten EM, Wiedlocha A (2011)
The driver of malignancy in KG-1a leukemic cells, FGFR1OP2-FGFR1, encodes an HSP90 addicted oncoprotein
Cell Signal. 2011 Nov;23(11):1758-66
Sørensen V, Zhen Y, Zakrzewska M, Haugsten EM, Wälchli S, Nilsen T, Olsnes S, Wiedlocha A (2008)
Phosphorylation of fibroblast growth factor (FGF) receptor 1 at Ser777 by p38 mitogen-activated protein kinase regulates translocation of exogenous FGF1 to the cytosol and nucleus.
Mol Cell Biol. 12, 4129-41
Zhen Y, Sørensen V, Jin Y, Suo Z, Wiedlocha A (2007)
Indirubin-3'-monoxime inhibits autophosphorylation of FGFR1 and stimulates ERK1/2 activity via p38 MAPK
Oncogene, 26 (44), 6372-85
Nilsen T, Rosendal KR, Sørensen V, Wesche J, Olsnes S, Wiedlocha A (2007)
A nuclear export sequence located on a beta-strand in fibroblast growth factor-1
J Biol Chem, 282 (36), 26245-56
Wiedlocha A, Nilsen T, Wesche J, Sørensen V, Malecki J, Marcinkowska E, Olsnes S (2005)
Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1
Mol Biol Cell, 16 (2), 794-810
Professor Jacek Otlewski
May 6, 2013
May 2, 2013
Protein internalisation gr.
ERK-mediated phosphorylation of fibroblast growth factor receptor 1 on Ser777 inhibits signaling
Sci Signal, 6 (262), ra11
FGF1-gold nanoparticle conjugates targeting FGFR efficiently decrease cell viability upon NIR irradiation
Int J Nanomedicine, 7, 5915-27
Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration
EMBO Rep, 14 (1), 57-64