Microsphere Affinity Proteomics

 
Group leader Fridtjof Lund-Johansen
Group leader Fridtjof Lund-Johansen

The goal of our research is to put proteomics into the hands of antibody users.

The analytical power of mass spectrometry (MS) is immense, but it is not realistic to expect that our MS core facilities can be built to handle more than a few samples per day. The large majority of research on proteins is therefore based on detection methods such as western blotting where one or a few proteins are measured in each experiment.

In microsphere affinity proteomics (MAP) antibodies and recombinant proteins are bound to microspheres with thousands of fluorescent bar codes. A flow cytometer is used to read the bar codes and measure fluorescence from sample proteins captured onto the microsphere surface. With MAP technology, scientists can detect thousands of proteins in multiple samples on a daily basis.

Current applications include large-scale detection of protein complexes in subcellular fractions, detection of protein interaction partners and measurement of protein phosphorylation. MAP protein arrays provide means to rapidly identify the targets of auto-antibodies or determine the specificity of antibodies used for research purpose.

Projects

  • An antibody array "western blot"
  • Large-scale analysis of protein complexes in subcellular compartments
  • Bead-based protein arrays

Contact information:
Group leader Fridtjof Lund-Johansen, Department of Immunology, Oslo University Hospital, Rikshospitalet, Tel: +47 23073016, E-mail: fridtjof.lund-johansen@rr-research.no