Isoflurane and sevoflurane: Effects on mitochondrial function in the rat and human brain
1. Sevoflurane slowly depolarizes the mitochondrial membrane in isolated CNS presynaptic terminals, and this effect is not dependent on Ca2+-influx to the cytosol. Opening of mitoKATP is only partly responsible for this depolarizing effect of sevoflurane.
2. Isoflurane and sevoflurane depolarize presynaptic mitochondria through inhibition of the electron transport chain. Isoflurane seems to inhibit mitochondrial function more pronounced than sevoflurane. Both agents inhibit the respiratory chain sufficiently to cause ATP synthase reversal.
3. Isoflurane depolarizes the mitochondrial membrane potential (Δψm) of neural mitochondria in an age dependent manner by inhibition of the respiratory chain. The effect is more pronounced in the adolescent and adult than in neonatal rats.
4. Sevoflurane and propofol at equipotent doses depolarize the mitochondria in rat and human nerve terminals to the same extent. The depolarizing effect of propofol on the Δψm was more rapid in onset than that of sevoflurane. Whereas sevoflurane inhibits the respiratory chain sufficiently to cause ATP synthase reversal, the depolarizing effect of propofol seems to be related to inhibition of the respiratory chain from complex I-V.
Papers included in the thesis
Sevoflurane depolarizes pre-synaptic mitochondria in the central nervous system
Acta Anaesthesiol Scand, 48 (5), 562-8
Reply: The Importance of a Skin Bridge in Peripheral Tissue Perfusion in Perforator Flaps
Plast. Reconstr. Surg., 130 (5), 758E-760E
The importance of a skin bridge in peripheral tissue perfusion in perforator flaps
Plast Reconstr Surg, 129 (3), 428e-434e
Isoflurane-induced depolarization of neural mitochondria increases with age
Acta Anaesthesiol Scand, 53 (1), 85-92
[Hand function after surgery for flexor tendon injuries]
Tidsskr Nor Laegeforen, 128 (1), 36-8