Receptor signalling and trafficking
1. Research group name/project:
Receptor signalling and trafficking
2. Group leader and some key members (incl. from other depts./inst.):
Harald Stenmark, prof. (group leader), Andreas Brech, Ph,D. (electron microscopy), Tor Erik Rusten, Ph.D. (autophagy and endocytic membrane trafficking in Drosophila), Thomas Slagsvold, Ph.D. (endocytic trafficking in mammalian cells), Camilla Raiborg, M.Sc. (endocytic trafficking in mammalian cells), Kristi Bache, M.Sc. (endocytic trafficking in mammalian cells).
3. Home address on the internet:
http://www.radium.no/stenmark
4. Department/Institute:
Department of Biochemistry, Institute for Cancer Research, the Norwegian Radium Hospital
5. Main aim of research group:
To understand how intracellular receptor trafficking regulates intercellular communication.
6. Some important recent results (with a few key references):
We discovered the conserved FYVE zinc finger domain (Stenmark et al, J Biol Chem, 1996) and found that it interacts with PtdIns 3-phosphate (Gaullier et al, Nature, 1998). This lipid is restricted to endosomes (Gillooly et al, EMBO J, 2000) where it functions to recruit FYVE finger proteins. Two of these control distinct steps of endocytic trafficking: EEA1 regulates Rab5-dependent endosome fusion (Simonsen et al, Nature 1998) whereas Hrs mediates sorting of ubiquitinated membrane proteins from endosomes to lysosomes (Raiborg et al, EMBO J, 2001; Raiborg et al, Nature Cell Biol, 2002). Hrs also serves to recruit a protein complex required for the biogenesis of multivesicular endosomes (Bache et al., J Cell Biol, 2003).
7. Methods in current use:
Standard molecular biology methods, confocal and electron microscopy, protein expression and purification, Drosophila methods, various assays for cell signalling and intracellular trafficking, various assays for protein-protein and protein-lipid interactions.
8. Available equipment:
Zeiss LSM-510 Meta confocal microscope, Leica TCS NT confocal microscope, Zeiss Axiovert 200 fluorescence microscope, Leica MZ FLIII and Leica MZ12,5 stereomicroscopes, Eppendorf Femtojet microinjector, Biacore-X SPR analyzer, ÃKTA Explorer FPLC system, Origin Analyzer electrochemoluminescence system, Beckman ultracentrifuge, Beckman Table Top ultracentrifuge.
9. Collaborators:
9.1. Among Helse Sør hospitals : Inger Helene Madshus and Espen Stang (Rikshospitalet)
9.2. Other Norwegian collaborators: Kristian Prydz (Dept. of Bioscience, Oslo), Rein Aasland (Univ. of Bergen)
9.3. Collaborators from other countries: Marino Zerial (Max-Planck Institute for Molecular Cell Biology and Genetics, Dresden). Gergely Lukacs, The Hospital for Sick Children, Toronto. Kim Finley, Salk Institute, La Jolla. In addition, we collaborate with partners within a Research Training Network under the EU 5th framework programme, “Membrane-cytoskeleton interactions in intracellular transport and cell morphogenesis”.
10. Is the group interested in joining a larger collaborative project in Helse Sør?
Yes
11. Tentative name of possible collaborative project(s):
Growth factor receptors and cancer
12. Some key search words:
Endocytosis, membrane traffic, autophagy, signalling, growth factor, receptor, cancer, Drosophila
Receptor signalling and trafficking
2. Group leader and some key members (incl. from other depts./inst.):
Harald Stenmark, prof. (group leader), Andreas Brech, Ph,D. (electron microscopy), Tor Erik Rusten, Ph.D. (autophagy and endocytic membrane trafficking in Drosophila), Thomas Slagsvold, Ph.D. (endocytic trafficking in mammalian cells), Camilla Raiborg, M.Sc. (endocytic trafficking in mammalian cells), Kristi Bache, M.Sc. (endocytic trafficking in mammalian cells).
3. Home address on the internet:
http://www.radium.no/stenmark
4. Department/Institute:
Department of Biochemistry, Institute for Cancer Research, the Norwegian Radium Hospital
5. Main aim of research group:
To understand how intracellular receptor trafficking regulates intercellular communication.
6. Some important recent results (with a few key references):
We discovered the conserved FYVE zinc finger domain (Stenmark et al, J Biol Chem, 1996) and found that it interacts with PtdIns 3-phosphate (Gaullier et al, Nature, 1998). This lipid is restricted to endosomes (Gillooly et al, EMBO J, 2000) where it functions to recruit FYVE finger proteins. Two of these control distinct steps of endocytic trafficking: EEA1 regulates Rab5-dependent endosome fusion (Simonsen et al, Nature 1998) whereas Hrs mediates sorting of ubiquitinated membrane proteins from endosomes to lysosomes (Raiborg et al, EMBO J, 2001; Raiborg et al, Nature Cell Biol, 2002). Hrs also serves to recruit a protein complex required for the biogenesis of multivesicular endosomes (Bache et al., J Cell Biol, 2003).
7. Methods in current use:
Standard molecular biology methods, confocal and electron microscopy, protein expression and purification, Drosophila methods, various assays for cell signalling and intracellular trafficking, various assays for protein-protein and protein-lipid interactions.
8. Available equipment:
Zeiss LSM-510 Meta confocal microscope, Leica TCS NT confocal microscope, Zeiss Axiovert 200 fluorescence microscope, Leica MZ FLIII and Leica MZ12,5 stereomicroscopes, Eppendorf Femtojet microinjector, Biacore-X SPR analyzer, ÃKTA Explorer FPLC system, Origin Analyzer electrochemoluminescence system, Beckman ultracentrifuge, Beckman Table Top ultracentrifuge.
9. Collaborators:
9.1. Among Helse Sør hospitals : Inger Helene Madshus and Espen Stang (Rikshospitalet)
9.2. Other Norwegian collaborators: Kristian Prydz (Dept. of Bioscience, Oslo), Rein Aasland (Univ. of Bergen)
9.3. Collaborators from other countries: Marino Zerial (Max-Planck Institute for Molecular Cell Biology and Genetics, Dresden). Gergely Lukacs, The Hospital for Sick Children, Toronto. Kim Finley, Salk Institute, La Jolla. In addition, we collaborate with partners within a Research Training Network under the EU 5th framework programme, “Membrane-cytoskeleton interactions in intracellular transport and cell morphogenesis”.
10. Is the group interested in joining a larger collaborative project in Helse Sør?
Yes
11. Tentative name of possible collaborative project(s):
Growth factor receptors and cancer
12. Some key search words:
Endocytosis, membrane traffic, autophagy, signalling, growth factor, receptor, cancer, Drosophila
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