Intracellular transport of protein toxins
1. Research group name/project:
Intracellular transport of protein toxins
2. Group leader and some key members (incl. from other depts./inst.):
Kirsten Sandvig (prof), T.G. Iversen, H.H. Slagsvold, S. Wälchli , T. Gregers, M. Slominska , S. Grimmer, M.L. Torgersen, S.U. Lauvrak, B. Spilsberg, A. Utskarpen, A.G. Myrann, E. Rolén
3. Home address on the internet:
http://radium.no/sandvig/
4. Department/Institute:
Dept. of Biochemistry, Inst. for Cancer Research, The Norwegian Radium Hospital
5. Main aim of research group:
To investigate the mechanisms and pathways of endocytosis and intracellular transport. Studies of protein toxins and their mechanisms of action with the aim of using them as tools to clarify transport and with the aim of using them as tools in cancer treatment.
6. Some important recent results (with a few key references):
Molecular biology techniques, Confocal and electron microscopy.
8. Available equipment:
Confocal microscopes and standard molecular biology equipment
9. Collaborators:
9.1. Among Helse Sør hospitals :
9.2. Other Norwegian collaborators: Collaborators vary depending on the exact question)
9.3. Collaborators from other countries: EM, Bo van Deurs in Copenhagen; Shiga toxin, Yuri Kozlov in Moscow, a number of other collaborators depending on the question (see reference list)
10. Some key search words:
Toxins, ricin, Shiga toxin, diphtheria toxin, endocytosis, Golgi, endoplasmic reticulum, membranes, lipid rafts, cholesterol.
Intracellular transport of protein toxins
2. Group leader and some key members (incl. from other depts./inst.):
Kirsten Sandvig (prof), T.G. Iversen, H.H. Slagsvold, S. Wälchli , T. Gregers, M. Slominska , S. Grimmer, M.L. Torgersen, S.U. Lauvrak, B. Spilsberg, A. Utskarpen, A.G. Myrann, E. Rolén
3. Home address on the internet:
http://radium.no/sandvig/
4. Department/Institute:
Dept. of Biochemistry, Inst. for Cancer Research, The Norwegian Radium Hospital
5. Main aim of research group:
To investigate the mechanisms and pathways of endocytosis and intracellular transport. Studies of protein toxins and their mechanisms of action with the aim of using them as tools to clarify transport and with the aim of using them as tools in cancer treatment.
6. Some important recent results (with a few key references):
- Characterization of previously unknown transport pathways in cells. The studies have been performed by using protein toxins (for review, see Sandvig, K. and van Deurs, B. 2002.
- Membrane Traffic Exploited by Protein Toxins. Ann.Rev. Cell Dev. Biol. 18, 1-24. Recent studies include: Lauvrak, S.U., Llorente, A., Iversen, T.-G., and Sandvig, K. 2002.
- Selective regulation of the Rab9-independent transport of ricin to the Golgi apparatus by calcium. J.Cell Sci. 115, 3449-3456; Birkeli, K. A., Llorente, A., Torgersen, M.L., Keryer, G., Taskén, K., and Sandvig, K. 2003. J. Biol.Chem. 278, 1991-1997; Iversen, T.-G., Skretting, G., van Deurs, B. and Sandvig, K. 2003.
- Clathrin-coated pits with long, dynamin-wrapped necks upon expression of a clathrin antisense RNA. Proc.Natl.Acad.Sci. USA 100, 5175-5180; Spilsberg, B., van Meer, G., and Sandvig, K. 2003.
- Role of lipids in the retrograde pathway of ricin intoxicaton. Traffic. 4, 544-552; Llorente, A., et al, K. 2003. J. Biol. Chem., 278, 35850-35855.
Molecular biology techniques, Confocal and electron microscopy.
8. Available equipment:
Confocal microscopes and standard molecular biology equipment
9. Collaborators:
9.1. Among Helse Sør hospitals :
9.2. Other Norwegian collaborators: Collaborators vary depending on the exact question)
9.3. Collaborators from other countries: EM, Bo van Deurs in Copenhagen; Shiga toxin, Yuri Kozlov in Moscow, a number of other collaborators depending on the question (see reference list)
10. Some key search words:
Toxins, ricin, Shiga toxin, diphtheria toxin, endocytosis, Golgi, endoplasmic reticulum, membranes, lipid rafts, cholesterol.
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