Fridtjof Lund-Johansen
Position: Group leader, MD PhD
Phone: +47 23073016
Email:

MD PhD, Leading a group working on array-based protein analysis. Our goal is to make proteomics simple and accessible. In Microsphere Affinity Proteomics (MAP) biotinylated sample proteins are captured onto bead-based antibody arrays and detected by flow cytometry. MAP has a multiplexing capacity similar to that of mass spectrometry but the throughput is at least two orders of magnitude higher. We are currently developing a multiplexed version of the western blot and complete set of MAP assays to analyze proteins and protein complexes in subcellular compartments. We are also developing a MAP array with thousands of recombinant human proteins. The MAP protein array will be used to test the specificity of antibodies to human proteins and to screen for autoantibodies in serum from patients with auto-immune disease and cancer. To validate MAP, we make extensive use of mass spectrometry. We generate reference data sets that are as lookup tables to identify antibody targets that correspond to specific binding or cross-reactivity, respectively. MAP technology is a game changer in antibody validation and protein detection. Lund-Johansen and co-workers have published work on MAP technology published in Molecular&Cellular Proteomics (2009, 2016), Proteomics (2011), New Biotechnology (2012)and Cytometry (2012). More recently, the group has published new bioinformatics for subcellular proteomics (Nature Methods in press)

 

Author network for Fridtjof Lund-Johansen by COREMINE medical


Publications 2017

Vesterhus M, Holm A, Hov JR, Nygård S, Schrumpf E, Melum E, Thorbjørnsen LW, Paulsen V, Lundin K, Dale I, Gilja OH, Zweers SJ, Vatn M, Schaap FG, Jansen PL, Ueland T, Røsjø H, Moum B, Ponsioen CY, Boberg KM, Färkkilä M, Karlsen TH, Lund-Johansen F (2017)
Novel serum and bile protein markers predict primary sclerosing cholangitis disease severity and prognosis
J Hepatol (in press)
PubMed 28161472

Publications 2016

Freedman LP, Gibson MC, Bradbury AR, Buchberg AM, Davis D, Dolled-Filhart MP, Lund-Johansen F, Rimm DL (2016)
[Letter to the Editor] The need for improved education and training in research antibody usage and validation practices
Biotechniques, 61 (1), 16-8
PubMed 27401669

Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T (2016)
High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells
Mol Cell Proteomics, 15 (4), 1246-61
PubMed 26785729

Lund-Johansen F, de la Rosa Carrillo D, Mehta A, Sikorski K, Inngjerdingen M, Kalina T, Røysland K, de Souza GA, Bradbury AR, Lecrevisse Q, Stuchly J (2016)
MetaMass, a tool for meta-analysis of subcellular proteomics data
Nat Methods, 13 (10), 837-40
PubMed 27571551

Pauthner M, Yeung J, Ullman C, Bakker J, Wurch T, Reichert JM, Lund-Johansen F, Bradbury AR, Carter PJ, Melis JP (2016)
Antibody Engineering & Therapeutics, the annual meeting of The Antibody Society December 7-10, 2015, San Diego, CA, USA
MAbs, 8 (3), 617-52
PubMed 26909869

Strønen E, Toebes M, Kelderman S, van Buuren MM, Yang W, van Rooij N, Donia M, Böschen ML, Lund-Johansen F, Olweus J, Schumacher TN (2016)
Targeting of cancer neoantigens with donor-derived T cell receptor repertoires
Science, 352 (6291), 1337-41
PubMed 27198675

Publications 2015

Ferrara F, D'Angelo S, Gaiotto T, Naranjo L, Tian H, Gräslund S, Dobrovetsky E, Hraber P, Lund-Johansen F, Saragozza S, Sblattero D, Kiss C, Bradbury AR (2015)
Recombinant renewable polyclonal antibodies
MAbs, 7 (1), 32-41
PubMed 25530082

Vesterhus M, Hov JR, Holm A, Schrumpf E, Nygård S, Godang K, Andersen IM, Naess S, Thorburn D, Saffioti F, Vatn M, Gilja OH, Lund-Johansen F, Syversveen T, Brabrand K, Parés A, Ponsioen CY, Pinzani M, Färkkilä M, Moum B, Ueland T, Røsjø H, Rosenberg W, Boberg KM, Karlsen TH (2015)
Enhanced liver fibrosis score predicts transplant-free survival in primary sclerosing cholangitis
Hepatology, 62 (1), 188-97
PubMed 25833813

Publications 2014

Almagro JC, Gilliland GL, Breden F, Scott JK, Sok D, Pauthner M, Reichert JM, Helguera G, Andrabi R, Mabry R, Bléry M, Voss JE, Laurén J, Abuqayyas L, Barghorn S, Ben-Jacob E, Crowe JE, Huston JS, Johnston SA, Krauland E, Lund-Johansen F, Marasco WA, Parren PW, Xu KY (2014)
Antibody engineering and therapeutics, The Annual Meeting of the Antibody Society: December 8-12, 2013, Huntington Beach, CA
MAbs, 6 (3), 577-618
PubMed 24589717

Publications 2013

Kumari S, Wälchli S, Fallang LE, Yang W, Lund-Johansen F, Schumacher TN, Olweus J (2013)
Alloreactive cytotoxic T cells provide means to decipher the immunopeptidome and reveal a plethora of tumor-associated self-epitopes
Proc Natl Acad Sci U S A, 111 (1), 403-8
PubMed 24344295

Publications 2011

Holm A, Wu W, Lund-Johansen F (2011)
Antibody array analysis of labelled proteomes: how should we control specificity?
N Biotechnol, 29 (5), 578-85
PubMed 21840428

Slaastad H, Wu W, Goullart L, Kanderova V, Tjønnfjord G, Stuchly J, Kalina T, Holm A, Lund-Johansen F (2011)
Multiplexed immuno-precipitation with 1725 commercially available antibodies to cellular proteins
Proteomics, 11 (23), 4578-82
PubMed 21956872

Stuchlý J, Kanderová V, Fišer K, Cerná D, Holm A, Wu W, Hrušák O, Lund-Johansen F, Kalina T (2011)
An automated analysis of highly complex flow cytometry-based proteomic data
Cytometry A, 81 (2), 120-9
PubMed 22213549

Publications 2010

Abrahamsen IW, Stronen E, Wälchli S, Johansen JN, Kjellevoll S, Kumari S, Komada M, Gaudernack G, Tjonnfjord G, Toebes M, Schumacher TN, Lund-Johansen F, Olweus J (2010)
Targeting B cell leukemia with highly specific allogeneic T cells with a public recognition motif
Leukemia, 24 (11), 1901-9
PubMed 20844564

Di Niro R, Mesin L, Raki M, Zheng NY, Lund-Johansen F, Lundin KE, Charpilienne A, Poncet D, Wilson PC, Sollid LM (2010)
Rapid generation of rotavirus-specific human monoclonal antibodies from small-intestinal mucosa
J Immunol, 185 (9), 5377-83
PubMed 20935207

Publications 2009

Stronen E, Abrahamsen IW, Gaudernack G, Wälchli S, Munthe E, Buus S, Johansen FE, Lund-Johansen F, Olweus J (2009)
Dendritic cells engineered to express defined allo-HLA peptide complexes induce antigen-specific cytotoxic T cells efficiently killing tumour cells
Scand J Immunol, 69 (4), 319-28
PubMed 19284496

Publications 2008

Stamnaes J, Fleckenstein B, Lund-Johansen F, Sollid LM (2008)
The monoclonal antibody 6B9 recognizes CD44 and not cell surface transglutaminase 2
Scand J Immunol, 68 (5), 534-42
PubMed 18803608

Wu W, Slåstad H, de la Rosa Carrillo D, Frey T, Tjønnfjord G, Boretti E, Aasheim HC, Horejsi V, Lund-Johansen F (2008)
Antibody array analysis with label-based detection and resolution of protein size
Mol Cell Proteomics, 8 (2), 245-57
PubMed 18796702

Publications 2007

Fløisand Y, Normann AP, Heim S, Lund-Johansen F, Tjønnfjord GE (2007)
High expression of CD7 on CD34+ cells is not linked to deletion of derivative chromosome 9 or lack of dendritic cells in chronic myeloid leukaemia
Scand J Clin Lab Invest, 68 (2), 93-8
PubMed 17852828

Villa I, Kvale EO, Lund-Johansen F, Olweus J (2007)
Assay for monitoring in vitro selective depletion strategies in allogeneic stem cell transplantation
Cytotherapy, 9 (6), 600-10
PubMed 17882725

Publications 2006

Farkas L, Kvale EO, Lund-Johansen F, Jahnsen FL (2006)
Plasmacytoid dendritic cells induce a distinct cytokine pattern in virus-specific CD4+ memory T cells that is modulated by CpG oligodeoxynucleotides
Scand J Immunol, 64 (4), 404-11
PubMed 16970682

Kvale EO, Fløisand Y, Lund-Johansen F, Rollag H, Farkas L, Ghanekar S, Brandtzaeg P, Jahnsen FL, Olweus J (2006)
Plasmacytoid DCs regulate recall responses by rapid induction of IL-10 in memory T cells
Blood, 109 (8), 3369-76
PubMed 17158235

Sioud M, Fløisand Y, Forfang L, Lund-Johansen F (2006)
Signaling through toll-like receptor 7/8 induces the differentiation of human bone marrow CD34+ progenitor cells along the myeloid lineage
J Mol Biol, 364 (5), 945-54
PubMed 17049554

Publications 2005

Kvale EØ, Dalgaard J, Lund-Johansen F, Rollag H, Farkas L, Midtvedt K, Jahnsen FL, Brinchmann JE, Olweus J (2005)
CD11c+ dendritic cells and plasmacytoid DCs are activated by human cytomegalovirus and retain efficient T cell-stimulatory capability upon infection
Blood, 107 (5), 2022-9
PubMed 16269620

Publications 2004

Farkas L, Kvale EO, Johansen FE, Jahnsen FL, Lund-Johansen F (2004)
Plasmacytoid dendritic cells activate allergen-specific TH2 memory cells: modulation by CpG oligodeoxynucleotides
J Allergy Clin Immunol, 114 (2), 436-43
PubMed 15316529

Publications 2003

Dueland S, Sauer T, Lund-Johansen F, Ostenstad B, Tveit KM (2003)
Epidermal growth factor receptor inhibition induces trichomegaly
Acta Oncol, 42 (4), 345-6
PubMed 12899508

Publications 2002

Jahnsen FL, Farkas L, Lund-Johansen F, Brandtzaeg P (2002)
Involvement of plasmacytoid dendritic cells in human diseases
Hum. Immunol., 63 (12), PII S0198-8859(02)00759-0-1205

Jahnsen FL, Farkas L, Lund-Johansen F, Brandtzaeg P (2002)
Involvement of plasmacytoid dendritic cells in human diseases
Hum Immunol, 63 (12), 1201-5
PubMed 12480264

Publications 2001

Farkas L, Beiske K, Lund-Johansen F, Brandtzaeg P, Jahnsen FL (2001)
Plasmacytoid dendritic cells (natural interferon- alpha/beta-producing cells) accumulate in cutaneous lupus erythematosus lesions
Am J Pathol, 159 (1), 237-43
PubMed 11438470

Publications 2000

Jahnsen FL, Lund-Johansen F, Dunne JF, Farkas L, Haye R, Brandtzaeg P (2000)
Experimentally induced recruitment of plasmacytoid (CD123high) dendritic cells in human nasal allergy
J Immunol, 165 (7), 4062-8
PubMed 11034417

Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R (2000)
Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions
Cytometry, 39 (4), 250-9
PubMed 10738277

Publications 1999

Lund-Johansen F, Houck D, Hoffman R, Davis K, Olweus J (1999)
Primitive human hematopoietic progenitor cells express receptors for granulocyte-macrophage colony-stimulating factor
Exp Hematol, 27 (4), 762-72
PubMed 10210334

Lund-Johansen F, Olweus J (1999)
[Dendritic cells--strong candidates for immunotherapy]
Tidsskr Nor Laegeforen, 119 (17), 2510-4
PubMed 10425906

Publications 1998

Solvason N, Wu WW, Kabra N, Lund-Johansen F, Roncarolo MG, Behrens TW, Grillot DA, Nunez G, Lees E, Howard M (1998)
Transgene expression of bcl-xL permits anti-immunoglobulin (Ig)-induced proliferation in xid B cells
J Exp Med, 187 (7), 1081-91
PubMed 9529324

Publications 1997

Olweus J, BitMansour A, Warnke R, Thompson PA, Carballido J, Picker LJ, Lund-Johansen F (1997)
Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin
Proc Natl Acad Sci U S A, 94 (23), 12551-6
PubMed 9356487

Publications 1996

Lund-Johansen F, Frey T, Ledbetter JA, Thompson PA (1996)
Apoptosis in hematopoietic cells is associated with an extensive decrease in cellular phosphotyrosine content that can be inhibited by the tyrosine phosphatase antagonist pervanadate
Cytometry, 25 (2), 182-90
PubMed 8891448

Olweus J, Terstappen LW, Thompson PA, Lund-Johansen F (1996)
Expression and function of receptors for stem cell factor and erythropoietin during lineage commitment of human hematopoietic progenitor cells
Blood, 88 (5), 1594-607
PubMed 8781415

Olweus J, Thompson PA, Lund-Johansen F (1996)
Granulocytic and monocytic differentiation of CD34hi cells is associated with distinct changes in the expression of the PU.1-regulated molecules, CD64 and macrophage colony-stimulating factor receptor
Blood, 88 (10), 3741-54
PubMed 8916938

Publications 1995

Abrahamsen JF, Lund-Johansen F, Laerum OD, Schem BC, Sletvold O, Smaaland R (1995)
Flow cytometric assessment of peripheral blood contamination and proliferative activity of human bone marrow cell populations
Cytometry, 19 (1), 77-85
PubMed 7705188

Brackman D, Lund-Johansen F, Aarskog D (1995)
Expression of cell surface antigens during the differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D3, retinoic acid and DMSO
Leuk Res, 19 (1), 57-64
PubMed 7837818

Brackman D, Lund-Johansen F, Aarskog D (1995)
Expression of leukocyte differentiation antigens during the differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D3: comparison with the maturation of normal monocytic and granulocytic bone marrow cells
J Leukoc Biol, 58 (5), 547-55
PubMed 7595056

Olweus J, Lund-Johansen F, Terstappen LW (1995)
CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells
Blood, 85 (9), 2402-13
PubMed 7537112

Waller EK, Olweus J, Lund-Johansen F, Huang S, Nguyen M, Guo GR, Terstappen L (1995)
The "common stem cell" hypothesis reevaluated: human fetal bone marrow contains separate populations of hematopoietic and stromal progenitors
Blood, 85 (9), 2422-35
PubMed 7537114

Publications 1994

Olweus J, Lund-Johansen F, Terstappen LW (1994)
Expression of cell surface markers during differentiation of CD34+, CD38-/lo fetal and adult bone marrow cells
Immunomethods, 5 (3), 179-88
PubMed 7540098

Terstappen LW, Lund-Johansen F (1994)
Commentary: hematopoietic progenitors in fetal and adult tissue
Blood Cells, 20 (2-3), 392-6
PubMed 7538344

Publications 1993

Lund-Johansen F, Olweus J (1993)
Use of monoclonal antibodies to study hematopoietic cell function
J Hematother, 2 (3), 395-412
PubMed 7922002

Lund-Johansen F, Olweus J, Symington FW, Arli A, Thompson JS, Vilella R, Skubitz K, Horejsi V (1993)
Activation of human monocytes and granulocytes by monoclonal antibodies to glycosylphosphatidylinositol-anchored antigens
Eur J Immunol, 23 (11), 2782-91
PubMed 8223854

Lund-Johansen F, Terstappen LW (1993)
Differential surface expression of cell adhesion molecules during granulocyte maturation
J Leukoc Biol, 54 (1), 47-55
PubMed 7687642

Olweus J, Lund-Johansen F, Horejsi V (1993)
CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells
J Immunol, 151 (2), 707-16
PubMed 8335905

Terstappen LW, Gandour D, Huang S, Lund-Johansen F, Manion K, Nguyen M, Mickaels R, Olweus J, Töpker S (1993)
Assessment of hematopoietic cell differentiation by multidimensional flow cytometry
J Hematother, 2 (3), 431-47
PubMed 7522894

Publications 1992

Lund-Johansen F, Olweus J (1992)
Signal transduction in monocytes and granulocytes measured by multiparameter flow cytometry
Cytometry, 13 (7), 693-702
PubMed 1451600

Lund-Johansen F, Olweus J, Horejsi V, Skubitz KM, Thompson JS, Vilella R, Symington FW (1992)
Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65)
J Immunol, 148 (10), 3221-9
PubMed 1349618

Publications 1991

Flø RW, Naess A, Lund-Johansen F, Maehle BO, Sjursen H, Lehmann V, Solberg CO (1991)
Negative selection of human monocytes using magnetic particles covered by anti-lymphocyte antibodies
J Immunol Methods, 137 (1), 89-94
PubMed 2010620

Lund-Johansen F, Olweus J, Aarli A, Bjerknes R (1991)
The IgG FcRII and the PI-linked IgG FcRIII trigger cytoplasmic calcium fluxes independently in human granulocytes
Scand J Immunol, 33 (3), 261-6
PubMed 1826373

Publications 1990

Lund-Johansen F, Bjerknes R, Laerum OD (1990)
Flow cytometric assay for the measurement of human bone marrow phenotype, function and cell cycle
Cytometry, 11 (5), 610-6
PubMed 2379452

Lund-Johansen F, Olweus J, Aarli A, Bjerknes R (1990)
Signal transduction in human monocytes and granulocytes through the PI-linked antigen CD14
FEBS Lett, 273 (1-2), 55-8
PubMed 1699812