Sébastien Wälchli
Position: Senior Scientist, PhD
Phone: +47 22 78 13 16
Email:

Project leader

Education:

1998-2002: PhD in molecular biology (Serono Pharmaceutical Research Institute (Dept. of Genomics) and University of Geneva), "Substrate Recognition of Protein Tyrosine Phosphatases & PTP-SAP1, a Prototype Single Catalytic Domain Phosphatase"

1996-1997: Master in biology (University of Geneva, Medical Faculty), "Description of two new genes involved in the heat-shock response in E. coli (trxC and prpC)"

1993-1996: Bachelor in biology (University of Geneva)

Research interests/projects:

  • A Molecular Biology facility for the lymphoma group:
    • Expression and inhibition of genes involved in lymphoma development
    • Manipulation of B cells with retrovirus
  • A Molecular Biology facility for discovery and improvement of immunotherapeutic molecules
    • Therapeutic TcRs discovery, cloning, design and expression
    • Chimeric Antigen Receptors (CAR) discovery, cloning, design and improvement
    • Discovery and validation of vaccination targets
    • Development of novel technologies to improve cell-based therapy

Work experience

2013-present: 50% Researcher at Department of Immunology, IKF, and 50 % Senior Researcher at the Department of Cellular Therapy, KKT, The Norwegian Radium Hospital
2007-2013: Researcher at Department of Immunology, IKF, The Norwegian Radium Hospital 
2003-2007: Postdoctoral fellow at Department of Biochemistry, IKF, The Norwegian Radium Hospital

Awards

  • Inven2 Innovation award 2014: "A universal killer-T cell for personalized medicine"
  • Top poster Award at CIMT (Association of Cancer Immunotherapy) in Mainz (Germany) 2012: "It takes two to tango: MHC-I and Invariant chain in harmony"
  • Inven2 Innovation award 2011: "A novel method to produce soluble TcR"
 

Author network for Sébastien Wälchli by COREMINE medical


Publications 2017

Bollum LK, Huse K, Oksvold MP, Bai B, Hilden VI, Forfang L, Yoon SO, Wälchli S, Smeland EB, Myklebust JH (2017)
BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-β receptor type I ALK5
PLoS One, 12 (5), e0177188
PubMed 28489883

Inderberg EM, Wälchli S, Myhre MR, Trachsel S, Almåsbak H, Kvalheim G, Gaudernack G (2017)
T cell therapy targeting a public neoantigen in microsatellite instable colon cancer reduces in vivo tumor growth
Oncoimmunology, 6 (4), e1302631
PubMed 28507809

Koksal H, Walchli S (2017)
Fishing therapeutic T-cell receptors in healthy donor blood, is safety predictable?
Transl. Cancer Res., 6 3, S622-S624

Walseng E, Köksal H, Sektioglu IM, Fåne A, Skorstad G, Kvalheim G, Gaudernack G, Inderberg EM, Wälchli S (2017)
A TCR-based Chimeric Antigen Receptor
Sci Rep, 7 (1), 10713
PubMed 28878363

Publications 2016

Belnoue E, Di Berardino-Besson W, Gaertner H, Carboni S, Dunand-Sauthier I, Cerini F, Suso-Inderberg EM, Wälchli S, König S, Salazar AM, Hartley O, Dietrich PY, Walker PR, Derouazi M (2016)
Enhancing Antitumor Immune Responses by Optimized Combinations of Cell-penetrating Peptide-based Vaccines and Adjuvants
Mol Ther, 24 (9), 1675-85
PubMed 27377043

Kyte JA, Gaudernack G, Faane A, Lislerud K, Inderberg EM, Brunsvig P, Aamdal S, Kvalheim G, Wälchli S, Pule M (2016)
T-helper cell receptors from long-term survivors after telomerase cancer vaccination for use in adoptive cell therapy
Oncoimmunology, 5 (12), e1249090
PubMed 28123886

Mensali N, Ying F, Sheng VO, Yang W, Walseng E, Kumari S, Fallang LE, Kolstad A, Uckert W, Malmberg KJ, Wälchli S, Olweus J (2016)
Targeting B-cell neoplasia with T-cell receptors recognizing a CD20-derived peptide on patient-specific HLA
Oncoimmunology, 5 (5), e1138199
PubMed 27467957

Myklebust JH, Brody J, Kohrt HE, Kolstad A, Czerwinski DK, Wälchli S, Green MR, Trøen G, Liestøl K, Beiske K, Houot R, Delabie J, Alizadeh AA, Irish JM, Levy R (2016)
Distinct patterns of B-cell receptor signaling in non-Hodgkin lymphomas identified by single-cell profiling
Blood, 129 (6), 759-770
PubMed 28011673

Publications 2015

Tadesse FG, Mensali N, Fallang LE, Walseng E, Yang W, Olweus J, Wälchli S (2015)
Unpredicted phenotypes of two mutants of the TcR DMF5
J Immunol Methods, 425, 37-44
PubMed 26079729

Theodossiou TA, Wälchli S, Olsen CE, Skarpen E, Berg K (2015)
Deciphering the Nongenomic, Mitochondrial Toxicity of Tamoxifens As Determined by Cell Metabolism and Redox Activity
ACS Chem Biol, 11 (1), 251-62
PubMed 26569462

Walseng E, Wälchli S, Fallang LE, Yang W, Vefferstad A, Areffard A, Olweus J (2015)
Soluble T-cell receptors produced in human cells for targeted delivery
PLoS One, 10 (4), e0119559
PubMed 25875651

Publications 2013

Gregers TF, Skånland SS, Wälchli S, Bakke O, Sandvig K (2013)
BiP negatively affects ricin transport
Toxins (Basel), 5 (5), 969-82
PubMed 23666197

Kumari S, Wälchli S, Fallang LE, Yang W, Lund-Johansen F, Schumacher TN, Olweus J (2013)
Alloreactive cytotoxic T cells provide means to decipher the immunopeptidome and reveal a plethora of tumor-associated self-epitopes
Proc Natl Acad Sci U S A, 111 (1), 403-8
PubMed 24344295

Wälchli S, Kumari S, Fallang LE, Sand KM, Yang W, Landsverk OJ, Bakke O, Olweus J, Gregers TF (2013)
Invariant chain as a vehicle to load antigenic peptides on human MHC class I for cytotoxic T-cell activation
Eur J Immunol, 44 (3), 774-84
PubMed 24293164

Publications 2012

Huse K, Bakkebø M, Wälchli S, Oksvold MP, Hilden VI, Forfang L, Bredahl ML, Liestøl K, Alizadeh AA, Smeland EB, Myklebust JH (2012)
Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma
PLoS One, 7 (10), e46117
PubMed 23049692

Zhen Y, Sørensen V, Skjerpen CS, Haugsten EM, Jin Y, Wälchli S, Olsnes S, Wiedlocha A (2012)
Nuclear import of exogenous FGF1 requires the ER-protein LRRC59 and the importins Kpnα1 and Kpnβ1
Traffic, 13 (5), 650-64
PubMed 22321063

Publications 2011

Abrahamsen IW, Kjellevoll S, Greve-Isdahl M, Mensali N, Wälchli S, Kumari S, Loland BF, Egeland T, Kolstad A, Olweus J (2011)
T cells raised against allogeneic HLA-A2/CD20 kill primary follicular lymphoma and acute lymphoblastic leukemia cells
Int J Cancer, 130 (8), 1821-32
PubMed 21630262

Sokołowska I, Wälchli S, Węgrzyn G, Sandvig K, Słomińska-Wojewódzka M (2011)
A single point mutation in ricin A-chain increases toxin degradation and inhibits EDEM1-dependent ER retrotranslocation
Biochem J, 436 (2), 371-85
PubMed 21388347

Wälchli S, Løset GÅ, Kumari S, Johansen JN, Yang W, Sandlie I, Olweus J (2011)
A practical approach to T-cell receptor cloning and expression
PLoS One, 6 (11), e27930
PubMed 22132171

Publications 2010

Abrahamsen IW, Stronen E, Wälchli S, Johansen JN, Kjellevoll S, Kumari S, Komada M, Gaudernack G, Tjonnfjord G, Toebes M, Schumacher TN, Lund-Johansen F, Olweus J (2010)
Targeting B cell leukemia with highly specific allogeneic T cells with a public recognition motif
Leukemia, 24 (11), 1901-9
PubMed 20844564

Almåsbak H, Rian E, Hoel HJ, Pulè M, Wälchli S, Kvalheim G, Gaudernack G, Rasmussen AM (2010)
Transiently redirected T cells for adoptive transfer
Cytotherapy, 13 (5), 629-40
PubMed 21174490

Yamazaki T, Wälchli S, Fujita T, Ryser S, Hoshijima M, Schlegel W, Kuroda S, Maturana AD (2010)
Splice variants of enigma homolog, differentially expressed during heart development, promote or prevent hypertrophy
Cardiovasc Res, 86 (3), 374-82
PubMed 20097676

Publications 2009

Raa H, Grimmer S, Schwudke D, Bergan J, Wälchli S, Skotland T, Shevchenko A, Sandvig K (2009)
Glycosphingolipid requirements for endosome-to-Golgi transport of Shiga toxin
Traffic, 10 (7), 868-82
PubMed 19453975

Skånland SS, Wälchli S, Brech A, Sandvig K (2009)
SNX4 in complex with clathrin and dynein: implications for endosome movement
PLoS One, 4 (6), e5935
PubMed 19529763

Skånland SS, Wälchli S, Sandvig K (2009)
β-arrestins attenuate p38-mediated endosome to Golgi transport
Cell Microbiol, 11 (5), 796-807
PubMed 19159388

Stronen E, Abrahamsen IW, Gaudernack G, Wälchli S, Munthe E, Buus S, Johansen FE, Lund-Johansen F, Olweus J (2009)
Dendritic cells engineered to express defined allo-HLA peptide complexes induce antigen-specific cytotoxic T cells efficiently killing tumour cells
Scand J Immunol, 69 (4), 319-28
PubMed 19284496

Wälchli S, Aasheim HC, Skånland SS, Spilsberg B, Torgersen ML, Rosendal KR, Sandvig K (2009)
Characterization of clathrin and Syk interaction upon Shiga toxin binding
Cell Signal, 21 (7), 1161-8
PubMed 19289168

Publications 2008

Maturana AD, Wälchli S, Iwata M, Ryser S, Van Lint J, Hoshijima M, Schlegel W, Ikeda Y, Tanizawa K, Kuroda S (2008)
Enigma homolog 1 scaffolds protein kinase D1 to regulate the activity of the cardiac L-type voltage-gated calcium channel
Cardiovasc Res, 78 (3), 458-65
PubMed 18296710

Nygren MK, Døsen-Dahl G, Stubberud H, Wälchli S, Munthe E, Rian E (2008)
beta-catenin is involved in N-cadherin-dependent adhesion, but not in canonical Wnt signaling in E2A-PBX1-positive B acute lymphoblastic leukemia cells
Exp Hematol, 37 (2), 225-33
PubMed 19101069

Sørensen V, Zhen Y, Zakrzewska M, Haugsten EM, Wälchli S, Nilsen T, Olsnes S, Wiedlocha A (2008)
Phosphorylation of fibroblast growth factor (FGF) receptor 1 at Ser777 by p38 mitogen-activated protein kinase regulates translocation of exogenous FGF1 to the cytosol and nucleus
Mol Cell Biol, 28 (12), 4129-41
PubMed 18411303

Walchli S, Sioud M (2008)
Vector-based delivery of siRNAs: in vitro and in vivo challenges
Front Biosci, 13, 3488-93
PubMed 18508450

Publications 2007

Fujita T, Maturana AD, Ikuta J, Hamada J, Walchli S, Suzuki T, Sawa H, Wooten MW, Okajima T, Tatematsu K, Tanizawa K, Kuroda S (2007)
Axonal guidance protein FEZ1 associates with tubulin and kinesin motor protein to transport mitochondria in neurites of NGF-stimulated PC12 cells
Biochem Biophys Res Commun, 361 (3), 605-10
PubMed 17669366

Skånland SS, Wälchli S, Utskarpen A, Wandinger-Ness A, Sandvig K (2007)
Phosphoinositide-regulated retrograde transport of ricin: crosstalk between hVps34 and sorting nexins
Traffic, 8 (3), 297-309
PubMed 17319803

Torgersen ML, Wälchli S, Grimmer S, Skånland SS, Sandvig K (2007)
Protein kinase Cdelta is activated by Shiga toxin and regulates its transport
J Biol Chem, 282 (22), 16317-28
PubMed 17403690

Wälchli S, Skånland SS, Gregers TF, Lauvrak SU, Torgersen ML, Ying M, Kuroda S, Maturana A, Sandvig K (2007)
The Mitogen-activated protein kinase p38 links Shiga Toxin-dependent signaling and trafficking
Mol Biol Cell, 19 (1), 95-104
PubMed 17959827

Publications 2006

Bache KG, Stuffers S, Malerød L, Slagsvold T, Raiborg C, Lechardeur D, Wälchli S, Lukacs GL, Brech A, Stenmark H (2006)
The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor
Mol Biol Cell, 17 (6), 2513-23
PubMed 16554368

Huynh T, Wälchli S, Sioud M (2006)
Transcriptional targeting of small interfering RNAs into cancer cells
Biochem Biophys Res Commun, 350 (4), 854-9
PubMed 17034763

Slominska-Wojewodzka M, Gregers TF, Wälchli S, Sandvig K (2006)
EDEM is involved in retrotranslocation of ricin from the endoplasmic reticulum to the cytosol
Mol Biol Cell, 17 (4), 1664-75
PubMed 16452630

Utskarpen A, Slagsvold HH, Iversen TG, Wälchli S, Sandvig K (2006)
Transport of ricin from endosomes to the Golgi apparatus is regulated by Rab6A and Rab6A'
Traffic, 7 (6), 663-72
PubMed 16683916

Publications 2005

Grimmer S, Ying M, Wälchli S, van Deurs B, Sandvig K (2005)
Golgi vesiculation induced by cholesterol occurs by a dynamin- and cPLA2-dependent mechanism
Traffic, 6 (2), 144-56
PubMed 15634214

Hetz C, Russelakis-Carneiro M, Wälchli S, Carboni S, Vial-Knecht E, Maundrell K, Castilla J, Soto C (2005)
The disulfide isomerase Grp58 is a protective factor against prion neurotoxicity
J Neurosci, 25 (11), 2793-802
PubMed 15772339

Lauvrak SU, Wälchli S, Iversen TG, Slagsvold HH, Torgersen ML, Spilsberg B, Sandvig K (2005)
Shiga toxin regulates its entry in a Syk-dependent manner
Mol Biol Cell, 17 (3), 1096-109
PubMed 16371508

Sioud M, Wälchli S (2005)
Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs
Methods Mol Biol, 309, 83-91
PubMed 15990399

Spilsberg B, Sandvig K, Wälchli S (2005)
Reconstitution of active diphtheria toxin based on a hexahistidine tagged version of the B-fragment produced to high yields in bacteria
Toxicon, 46 (8), 900-6
PubMed 16260019

Wälchli S, Espanel X, Hooft van Huijsduijnen R (2005)
Sap-1/PTPRH activity is regulated by reversible dimerization
Biochem Biophys Res Commun, 331 (2), 497-502
PubMed 15850787

Publications 2003

Espanel X, Wälchli S, Rückle T, Harrenga A, Huguenin-Reggiani M, Hooft van Huijsduijnen R (2003)
Mapping of synergistic components of weakly interacting protein-protein motifs using arrays of paired peptides
J Biol Chem, 278 (17), 15162-7
PubMed 12551909

Pasquali C, Curchod ML, Wälchli S, Espanel X, Guerrier M, Arigoni F, Strous G, Hooft van Huijsduijnen R (2003)
Identification of protein tyrosine phosphatases with specificity for the ligand-activated growth hormone receptor
Mol Endocrinol, 17 (11), 2228-39
PubMed 12907755

Sozio MS, Mathis MA, Young JA, Wälchli S, Pitcher LA, Wrage PC, Bartók B, Campbell A, Watts JD, Aebersold R, Hooft van Huijsduijnen R, van Oers NS (2003)
PTPH1 is a predominant protein-tyrosine phosphatase capable of interacting with and dephosphorylating the T cell receptor zeta subunit
J Biol Chem, 279 (9), 7760-9
PubMed 14672952

Wälchli S, Espanel X, Harrenga A, Rossi M, Cesareni G, Hooft van Huijsduijnen R (2003)
Probing protein-tyrosine phosphatase substrate specificity using a phosphotyrosine-containing phage library
J Biol Chem, 279 (1), 311-8
PubMed 14578355

Publications 2002

Hooft van Huijsduijnen R, Wälchli S, Ibberson M, Harrenga A (2002)
Protein tyrosine phosphatases as drug targets: PTP1B and beyond
Expert Opin Ther Targets, 6 (6), 637-47
PubMed 12472377

Publications 2001

Espanel X, Wälchli S, Gobert RP, El Alama M, Curchod ML, Gullu-Isler N, Hooft van Huijsduijnen R (2001)
Pulling strings below the surface: hormone receptor signaling through inhibition of protein tyrosine phosphatases
Endocrine, 15 (1), 19-28
PubMed 11572321

Publications 2000

Wälchli S, Colinge J, Hooft van Huijsduijnen R (2000)
MetaBlasts: tracing protein tyrosine phosphatase gene family roots from Man to Drosophila melanogaster and Caenorhabditis elegans genomes
Gene, 253 (2), 137-43
PubMed 10940550

Wälchli S, Curchod ML, Gobert RP, Arkinstall S, Hooft van Huijsduijnen R (2000)
Identification of tyrosine phosphatases that dephosphorylate the insulin receptor. A brute force approach based on "substrate-trapping" mutants
J Biol Chem, 275 (13), 9792-6
PubMed 10734133